FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_DG4565_AP3_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_DG4565_AP3_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23917514
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT541470.22639058557674513TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG83500.035.24037648-49
CTCGTAT86450.031.95003544-45
TATCTCG90400.031.76248740-41
TCGTATG93100.031.75969144-45
CGTATGC99500.029.6452546-47
TCTCGTA96000.029.19223842-43
ACCGCTC123800.027.22186732-33
CGCTCAT126250.026.41134534-35
ATGCCGT113150.025.7120848-49
ATCTCGT113050.025.12564542-43
TCACCGC143300.023.31878330-31
CCGCTCA148900.022.90424232-33
CACCGCT149450.022.7564830-31
GTCACCG148950.022.64155428-29
TGCCGTC127050.022.00178550-51
GCCGTCT118350.021.41175750-51
GTATGCC141200.021.39483846-47
CCGTCTT118400.021.34251652-53
CGTCTGA179550.019.12679516-17
CATTATC161600.018.64987838-39
Produced by FastQC (version 0.11.5)