FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_DG4478_AP2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_DG4478_AP2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31815002
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG118250.027.65576740-41
AGAATCG144500.024.22606838-39
ATCGGGG128450.024.16544242-43
GAATCGG140200.023.91881240-41
CACTCCG178850.023.79594830-31
ACTCCGG200950.021.17889432-33
CCGGAGA222150.019.27533734-35
CGTCTGA233400.018.53989616-17
CGGAGAA242450.017.45574836-37
CTCCGGA249050.017.39368432-33
ACGTCTG248300.017.24560514-15
TCCGGAG250050.017.20062434-35
TCGGGGG151800.017.02199242-43
ACACGTC257300.016.6238512-13
GAGCACA556950.016.1443589
ATCGGAA529150.016.0969332
CACGTCT270850.015.88871514-15
GTCACTC276650.015.81298628-29
CGGAAGA549150.015.7356254
AGAGCAC565350.015.69461258
Produced by FastQC (version 0.11.5)