FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_DG4476_AM2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_DG4476_AM2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30633130
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG102950.029.2053340-41
ATCGGGG119000.024.42807442-43
AGAATCG137000.023.68015538-39
GAATCGG134050.023.51033840-41
CACTCCG166550.023.47148930-31
ACTCCGG180250.021.74010732-33
CCGGAGA206250.018.9534634-35
CGTCTGA212050.018.9169216-17
ACGTCTG225000.017.7120514-15
TCCGGAG229450.017.43037234-35
TCGGGGG140200.017.29547542-43
CTCCGGA232650.017.14980732-33
ACACGTC232250.017.1488912-13
ATCGGAA473300.016.8215872
CACGTCT244400.016.48101814-15
GATCGGA485950.016.2575171
CGGAGAA241450.016.20017636-37
GAGCACA525700.015.9657379
CGGAAGA505100.015.9601414
AGAGCAC525850.015.7806818
Produced by FastQC (version 0.11.5)