FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_DG4474_ADI2_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_DG4474_ADI2_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33493426
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT861900.2573340810223475TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG134050.036.65633848-49
TCGTATG144150.034.35156244-45
CTCGTAT136800.034.23543544-45
TATCTCG142050.033.4884840-41
CGTATGC151550.032.98763746-47
TCTCGTA155650.029.6468442-43
ACCGCTC194050.028.45533832-33
ATGCCGT176850.027.28814548-49
ATCTCGT176200.026.9844142-43
CGCTCAT205750.026.80259134-35
TCACCGC216150.025.74380530-31
CCGCTCA228900.024.41352832-33
CACCGCT230800.024.29494530-31
GTCACCG233550.023.91742728-29
TGCCGTC195650.023.73134250-51
GTATGCC210400.023.69309246-47
GCCGTCT185000.023.03064550-51
CCGTCTT188850.022.69944652-53
CGTCTGA254550.022.40154616-17
ATCGGAA531200.021.4000022
Produced by FastQC (version 0.11.5)