FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_DG4460_ADI1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_DG4460_ADI1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33002074
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGG383240.11612603498798288TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGG141100.030.65063940-41
CACTCCG201200.027.82192430-31
ATCGGGG152750.027.69102142-43
GAATCGG167350.026.83628540-41
AGAATCG172450.026.6899138-39
ACTCCGG230000.024.54463632-33
CGTCTGA249350.023.03071616-17
CCGGAGA245300.022.85881434-35
ATCGGAA508600.022.2580382
ACGTCTG259400.022.04690714-15
GATCGGA513700.021.9272541
ACACGTC265400.021.45894812-13
CGGAAGA542600.021.0909214
GAGCACA566900.020.75439
AGAGCAC565550.020.6863218
TCGGGGG170650.020.51386342-43
CTCCGGA278800.020.46140532-33
CACGTCT282500.020.41227514-15
TCCGGAG284050.020.24208534-35
TCGGAAG564800.020.2198073
Produced by FastQC (version 0.11.5)