FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_DG4450_AM1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_DG4450_AM1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31414823
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT743000.23651255332554316TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG136700.037.2139548-49
TACTCGA149850.034.94679636-37
TCGTATG147150.034.054744-45
CGTATGC151150.033.97053546-47
CTCGTAT134550.033.1663744-45
ACTCGAT147700.032.44861636-37
TTACTCG178450.030.34404634-35
TCTCGTA140250.029.58318142-43
ATGCCGT181400.027.74268548-49
ATCTCGT151950.026.91456242-43
CTCGATC164350.025.5920138-39
TGCCGTC193000.025.3984550-51
CGATCTC163350.025.18165640-41
GTATGCC205500.025.00922846-47
TCGATCT165650.024.58828438-39
CCGTCTT185900.024.50323152-53
GCCGTCT185350.024.43503250-51
GATCTCG171700.023.86021440-41
ATTACTC230600.023.8422334-35
ACATTAC244200.022.6602732-33
Produced by FastQC (version 0.11.5)