FastQCFastQC Report
Tue 9 Jun 2020
HNLNGBGXF_n01_DG4446_AP1_mRNA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNGBGXF_n01_DG4446_AP1_mRNA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31209170
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT699340.22408157602396986TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG123250.037.07430648-49
CTCGTAT116500.033.7997544-45
TACTCGA141800.033.26278736-37
TCGTATG139400.032.54059644-45
ACTCGAT136150.032.0091436-37
TTACTCG158600.030.96722234-35
CGTATGC149950.030.6629446-47
TCTCGTA128400.028.79909542-43
ATGCCGT163900.027.83577548-49
ATCTCGT143350.025.39801242-43
TGCCGTC174000.025.01889650-51
CTCGATC151950.024.61699738-39
GTATGCC189400.024.55204246-47
TCGATCT151700.024.26617838-39
CGATCTC152000.024.20267940-41
GCCGTCT171900.023.40412550-51
CCGTCTT173700.023.32565152-53
ATTACTC213050.023.28690734-35
GATCTCG157750.023.23015840-41
CGTCTGA235650.021.45698416-17
Produced by FastQC (version 0.11.5)