FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97633122
Sequences flagged as poor quality0
Sequence length151
%GC68

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG83456938.548014064325423No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA95750.092.2819061
CCTAGGT104700.086.1448754
TAGGTAG119450.078.2616
CTAGGTA118700.077.2507555
TTCCTAG119000.074.394622
TCCTAGG127550.070.313423
AGGTAGT157750.059.6749537
CCCTAGC265850.054.8461381
CCTAGCA278950.052.6333432
CTAGCAT323300.045.303353
TAGCATT389300.037.5245934
AGCATTG455600.032.1006055
TCCCTAG197350.031.1983642
CTCCCTA198950.031.1478231
GATCTGT171050.029.1286547
GAATATT206400.026.1381441
GTGAACA188900.025.5669779
ATTGTGA158000.025.4744268
GCATTGG148500.025.2000776
CTTGTAG198800.024.386981
Produced by FastQC (version 0.11.5)