FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-53_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-53_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12751641
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA851960.6681179308608202No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC521530.4089904977720123No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG373900.2932171631870753No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC330100.25886864286722No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT325450.25522205338120796No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC309150.24243938486034855No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT274890.2155722545827631No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA265210.20798107474951658No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT262240.205651962755225No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA252090.19769220290941375No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT239640.1879287536404138No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC232770.18254121175462829No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG231690.18169426193852226No Hit
AGAATAAACTCTGAACTCACTTTCCATCCAACTTTTGTTGTTTTTGTGGT230650.18087868063412388No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC228380.17909851759471584No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC218970.1717190752154958No Hit
TTTTATATACTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATT206480.1619242574347882No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA203600.1596657245918388No Hit
CTTATAATTAGAGAAAACAACAGAGTTGTTATTTCTAGTGATGTTCTTGT194150.15225491370091113No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT192750.15115701579114404No Hit
TTTATATACTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATTC191850.1504512242777224No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC189360.14849853442392238No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA184740.14487547132169107No Hit
CTACTAATGTTGTTATTAAAGTCTGTGAATTTCAATTTTGTAATGATCCA182240.14291493933996416No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG177910.13951929794761317No Hit
GACTAGAGACTAGTGGCAATAAAACAAGAAAAACAAACATTGTTCGTTTA176290.13824887322345414No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC174910.13716665956954088No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC174090.13652360507953445No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT171060.13414744031768147No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC169940.13326912198986782No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT169510.1329319104890108No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC168500.13213985556839314No Hit
GTATAAACCCACAAATGTAAGTGAAAAAACCCTCTTTAGAGTCATTTTCT166920.1309007993559417No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG166550.13061064062264613No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG163830.12847758182652727No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA160360.12575636343589033No Hit
ATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTG160110.12556031023771766No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC155850.12221956374085499No Hit
TCTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTT154980.12153729861121404No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT153440.12032961091047027No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA153000.11998455728168633No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA149790.117467234217149No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT147390.11558512351469118No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA145660.11422843538333616No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA142800.11198558679624059No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC142350.11163269103952973No Hit
GTCCATAAGAAAAGGCTGAGAGACATATTCAAAAGTGCAATTATTCGCAC142210.11152290124855303No Hit
GTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTG138420.10855073476425504No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA138350.1084958398687667No Hit
GTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAA138160.10834683943815546No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG136860.10732736280765748No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG135210.10603341169971772No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA134880.10577462147812976No Hit
GCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTC129910.10187708389845668No Hit
GTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAA129050.10120266089674262No Hit
GTACTAAGAGGTTTGATAACCCTGTCCTACCATTTAATGATGGTGTTTAT128290.10060665917429763No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAGG76400.0117.768753
TTCCTAG78400.0114.6388552
GTTCCTA81250.0111.031881
TAGGTAG82700.0109.6696556
CCTAGGT82600.0108.8258364
CTAGGTA88550.0102.083285
AGGTAGT113550.080.0739067
TCGGACT851.8189894E-1276.756294
GCCGACC6750.066.58999145
TATGCGC22650.065.28885
TGCGCTA22650.064.984317
AAATTAG218550.062.45635
ATGCGCT24200.060.8156476
CGCTAGT25000.058.591739
CTTATCG1550.056.1887661
TAGAAGG247950.054.3383649
CAGGTAT18550.051.193844
TCTGTTC135500.051.1615339
TGTGGGA11000.050.1184352
TATAAAT283050.049.8206862
Produced by FastQC (version 0.11.5)