FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-53_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-53_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13124126
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCTAGGTAGTAGAAATAGGAAAAGGAGTCCTCTTCCTATTTCTACCC1002990.7642337478320461No Hit
GTCCCAGAGTGGTTGCTATGAGGTTGCAGCAGCAGTCTGATGACAGAAGG445920.3397711969543724No Hit
GTGGAAGATCGTTGGTGTTATGTCCCAGAGTGGTTGCTATGAGGTTGCAG422860.32220050310397813No Hit
CTCGTATAGTTTCAACTTATTCCACTCCACTGGCTTTTCCTCAACAACGG353140.26907696558231764No Hit
GACTTATTCCCTGGGCTACTCTCGTATAGTTTCAACTTATTCCACTCCAC321980.24533443217475964No Hit
TCCTATTTCTACCCTTCCTGTCTCCTTCATGACTAAGACTTATTCCCTGG300650.22908192134089542No Hit
GTAGTAGAAATAGGAAAAGGAGTCCTCTTCCTATTTCTACCCTTCCTGTC287990.21943556469969888No Hit
TTTCTACCCTTCCTGTCTCCTTCATGACTAAGACTTATTCCCTGGGCTAC262490.2000056994271466No Hit
GGTAGGGAGAGATGTAGGATCAAAGTATTTGTTTCTAAAACATTGAAGAG262310.19986854743698743No Hit
ATTTCTACCCTTCCTGTCTCCTTCATGACTAAGACTTATTCCCTGGGCTA260990.19886276617582No Hit
GGTATATGCAGGGCATCCCAGAGCCCAAAGTGTTCTCCAGACCGTCTGCT253620.19324715413430196No Hit
ATCAAATGCCACCTGAAGGTCAAACATGACAAGAAAGAGTCCATTGGAAT252150.1921270795480019No Hit
ACATTGAAGAGACTTAAATTTTATCCGTTGTTGAGGAAAAGCCAGTGGAG246460.18779155274796966No Hit
GACTAAGACTTATTCCCTGGGCTACTCTCGTATAGTTTCAACTTATTCCA245790.1872810425623771No Hit
GTGGAGATAGTGGGCAGGCAGTTTGGCTGTGGAGTGAAAAAGAGAGAAAG229780.17508213499321784No Hit
GATGTAGGATCAAAGTATTTGTTTCTAAAACATTGAAGAGACTTAAATTT222580.16959605538685016No Hit
AAGTATTTGTTTCTAAAACATTGAAGAGACTTAAATTTTATCCGTTGTTG209790.15985064453053865No Hit
CCCAAAGACAGTGCAAAAGATGGAATACAGAAATTTTTTTTCTCTGGTCT206360.15723713716250515No Hit
GTGTTATGTCCCAGAGTGGTTGCTATGAGGTTGCAGCAGCAGTCTGATGA200610.15285589303241984No Hit
AGGTAGTAGAAATAGGAAAAGGAGTCCTCTTCCTATTTCTACCCTTCCTG198440.1512024495955007No Hit
CTCTCGTATAGTTTCAACTTATTCCACTCCACTGGCTTTTCCTCAACAAC197260.1503033421044571No Hit
GGAGAGATGTAGGATCAAAGTATTTGTTTCTAAAACATTGAAGAGACTTA193870.14772031295645896No Hit
AGGAAAAGGAGTCCTCTTCCTATTTCTACCCTTCCTGTCTCCTTCATGAC192200.14644784726998203No Hit
GAAAGGGAGTGAGTGGGTAGGGAGAGATGTAGGATCAAAGTATTTGTTTC190200.1449239362682132No Hit
GTTTCAACTTATTCCACTCCACTGGCTTTTCCTCAACAACGGATAAAATT189990.14476392561302748No Hit
ATGTAGGATCAAAGTATTTGTTTCTAAAACATTGAAGAGACTTAAATTTT188530.14365147058173625No Hit
CTTCAATGTTTTAGAAACAAATACTTTGATCCTACATCTCTCCCTACCCA184720.1407484201233667No Hit
CTCCCTACCCACTCACTCCCTTTCTCTCTTTTTCACTCCACAGCCAAACT181780.13850827095076654No Hit
CCACTGGCTTTTCCTCAACAACGGATAAAATTTAAGTCTCTTCAATGTTT178470.1359861982428392No Hit
GACTTAAATTTTATCCGTTGTTGAGGAAAAGCCAGTGGAGTGGAATAAGT168580.12845045833909244No Hit
CCATCAAATGCCACCTGAAGGTCAAACATGACAAGAAAGAGTCCATTGGA168180.12814567613873867No Hit
ACTTATTCCACTCCACTGGCTTTTCCTCAACAACGGATAAAATTTAAGTC166280.12669796068705833No Hit
ACAAGAAAGAGTCCATTGGAATTGCCCAGTGGAAGATCGTTGGTGTTATG156240.11904792745817894No Hit
GAAAAAGAGAGAAAGGGAGTGAGTGGGTAGGGAGAGATGTAGGATCAAAG154120.11743258179630399No Hit
ATTTAAGTCTCTTCAATGTTTTAGAAACAAATACTTTGATCCTACATCTC153100.11665538718540192No Hit
GTATTTGTTTCTAAAACATTGAAGAGACTTAAATTTTATCCGTTGTTGAG150580.11473525932317322No Hit
TTCCTCAACAACGGATAAAATTTAAGTCTCTTCAATGTTTTAGAAACAAA148340.11302847900119216No Hit
TTCCTAGGTAGTAGAAATAGGAAAAGGAGTCCTCTTCCTATTTCTACCCT144670.11023210231294639No Hit
GTCTGATGACAGAAGGCCAAGGAATGAGTGAGCTGGCGGGGAGTGGAGAT142280.10841102866583267No Hit
CTATCTCCACTCCCCGCCAGCTCACTCATTCCTTGGCCTTCTGTCATCAG137960.10511938090201206No Hit
GAATTGCCCAGTGGAAGATCGTTGGTGTTATGTCCCAGAGTGGTTGCTAT135770.10345069835507523No Hit
CTATGAGGTTGCAGCAGCAGTCTGATGACAGAAGGCCAAGGAATGAGTGA135360.10313829659971262No Hit
GAGATGTAGGATCAAAGTATTTGTTTCTAAAACATTGAAGAGACTTAAAT135280.10307734015964187No Hit
GGGTAGGGAGAGATGTAGGATCAAAGTATTTGTTTCTAAAACATTGAAGA134010.10210965667351868No Hit
GAAAAGGAGTCCTCTTCCTATTTCTACCCTTCCTGTCTCCTTCATGACTA131350.10008285504116617No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA362150.0126.218741
TCCTAGG402400.0113.5161743
TTCCTAG408550.0111.804592
CCTAGGT444300.0102.9266054
CTAGGTA475200.096.443685
TAGGTAG483350.095.3679666
AGGTAGT607950.075.794237
TTAGCAA98900.068.2957152
TGTAGAG159350.060.371442
TATATGC152050.058.605813
ATATGCA109450.055.5723154
TAGAGCA177300.055.036224
GTATATG160400.054.5937582
ATTAGCA126150.053.8025741
GTAGAGC182650.052.876143
TTGTAGA178850.052.479331
CGTGGAC24950.048.821813
TATGCAG102100.048.5653045
CTCGTGG66200.044.519131
CGCCGTT14150.044.060658145
Produced by FastQC (version 0.11.5)