FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-52_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-52_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12355129
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG476310.385516007157837No Hit
GTAGCAAAAGCTGCATATGATGGAAGGGAACTAAACTCTGAGGCTATAGC448470.3629828551365186No Hit
ATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTT384940.3115629144786752No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT374540.3031453576891022No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC371380.3005877154338089No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA359520.29098846317185356No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT351700.2846591079704631No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG324660.26277346031757337No Hit
CCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGT298180.2413410657225837No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC282720.22882804380269928No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC280600.22711215722636324No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC278030.22503204944278607No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG276480.2237775097289555No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG274380.22207781076183014No Hit
GTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTT274140.2218835594513016No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC260660.2109731108432781No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG236190.19116757097396556No Hit
GTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTAGA235410.1905362542147476No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG234370.18969449853579026No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT231840.18764676597063454No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC231320.1872258881311559No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA223380.18079940727450114No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA216790.1754655900395698No Hit
GTCTCAAGGGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGT203400.16462798567299458No Hit
CAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTT196270.1588571029893739No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA194470.15740021816040933No Hit
AATTAAATGTTGGTGATTATTTTGTGCTGACATCACATACAGTAATGCCA184820.14958969671623826No Hit
GTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACATCA184290.14916072507215425No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG183570.14857797114056842No Hit
GACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTG179720.14546185636750533No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT173140.1401361329371794No Hit
CATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGTTA172450.13957766041940964No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT172120.1393105648674328No Hit
CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTT168980.1367691102213502No Hit
TCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCTATTGAAACAC168240.13617016868055365No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG167110.1352555687601481No Hit
CTCAAGGGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTG165250.1337501211035514No Hit
AGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACAT160060.12954943651337028No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT154990.12544587757845344No Hit
GTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCT154250.12484693603765692No Hit
CCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAAC154190.12479837321002477No Hit
GTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTA153820.1244989024396265No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT150330.12167416463235634No Hit
GGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTA149740.12119663016064017No Hit
GTCCAACATTTTGCTTCAGACATAAAAACATTGTTTTGATAATAAAGAAC149450.12096190982708477No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT148800.12043581252773647No Hit
TTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGAATCACCATTAGC147270.11919746042311659No Hit
CTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCC147110.11906795954943084No Hit
CATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGT146290.11840426757179143No Hit
ATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAAGTAGT144470.11693119513361616No Hit
GACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCT144350.11683406947835186No Hit
GTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGA143110.1158304377072874No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC142950.11570093683360165No Hit
CCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCT142620.11543384128162482No Hit
GTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTT140460.11368557948686735No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG139650.11302998131383331No Hit
GTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTC139600.11298951229080652No Hit
ACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACT139550.11294904326777971No Hit
GCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTAC138600.11218013183027065No Hit
GTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAAC137400.11120887527762763No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT137360.11117650005920618No Hit
TCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAA136580.1105451832999882No Hit
GTATAGGCAGAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAG136030.11010002404669347No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT135680.10981674088550593No Hit
GTACTTTTCTGTGTCTTTGATTTCGAGCAACATAAGCCCGTTAATACAAA135100.10934730021839513No Hit
CACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATAC134880.10916923651707723No Hit
GAAGGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACC134710.10903164183878615No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA134600.1089426099881272No Hit
GTAGATAAAAGTGCATTAACATTGGCCGTGACAGCTTGACAAATGTTAAA133970.1084327002979896No Hit
CTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACT133720.10823035518285563No Hit
GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT132730.10742906852692513No Hit
GAGTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACTCATTCA131570.10649018719270353No Hit
GTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCA130890.10593980847953914No Hit
CACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATC130570.10568080673216766No Hit
GTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTCTCAGT129810.10506567758216041No Hit
GCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTA129370.10470955017952463No Hit
CAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGA129060.10445864223675852No Hit
CATAAAAACATTGTTTTGATAATAAAGAACTGACTTAAAGTTCTTTATGC126820.1026456300051582No Hit
GAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTCCCTGG124800.10101068147487574No Hit
CTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTTTG123850.10024177003736666No Hit
CTCCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAG123760.10016892579591845No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCAA32750.0103.0553051
AAGGGTA17900.0100.916062
TCCCTAG29600.0100.7312242
GCTCAAT28400.0100.644932
GGGTAGT75300.0100.1496051
AGGGTAG18850.096.9344253
TCAATGT29700.096.4119954
CCCTAGC87700.096.169031
GAAGGGT18900.095.623851
CCTAGCA89700.094.70622
CTCCCTA38750.076.6090851
GCCGACC3950.075.251015145
GGTAGTA100400.075.21962
GAATATT25500.073.72051
GACGAGG2800.069.9897461
ACGAGGT2950.066.398122
AGTAGAG117000.065.119465
GCTCGGA808.025381E-963.5092121
GAAGACA33100.060.6655584
GTAGTAG130100.058.3523373
Produced by FastQC (version 0.11.5)