FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-51_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-51_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10690815
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTGTTATTG2355002.202825509561245No Hit
CAATAACAGATGCGCAAACAGGTTCAGTAGTAGGCAGCTCTCCCTAGCAT1817201.6997768645327787No Hit
CCCTAGCATTGGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGCA1396971.3067011261536188No Hit
GCCTACTACTGAACCTGTTTGCGCATCTGTTATTGTGGGCTCAATGTGTC1265241.183483204975486No Hit
CCCTAGCATTGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGCAA1139661.0660178854465259No Hit
CATGAAGACAGTGTGGTATAGGCACAAAGATAGAAAATAAATCAGTGAAA939240.8785485484502351No Hit
CCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTACTACTGAACCT815700.7629914089805128No Hit
GCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTGTTATTGTG725780.6788818251929343No Hit
CACAATAACAGATGCGCAAACAGGTTCAGTAGTAGGCAGCTCTCCCTAGC663270.6204110724954084No Hit
CTACTGAACCTGTTTGCGCATCTGTTATTGTGGGCTCAATGTGTCCAGGC634420.5934252907753057No Hit
GTGTGGGACTGGGTTCCCGGGTTCGTTTCTAAAGAAAGATAACCTACTGT589240.5511647147574811No Hit
GGTTCACAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATC523710.4898691072663777No Hit
GCATTGGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGCAAACAG511980.4788970719257606No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCAGTAGTAGGCAGCTCTCC496270.4642022147048658No Hit
GCATTGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGCAAACAGG491740.4599649325144996No Hit
GTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTACTACTG478570.4476459465438323No Hit
CCAATATTCCAGGCACCAATGCTAGGGAGAGCTGCCTACTACTGAACCTG468950.43864756802919136No Hit
CCCAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTGTT460680.4309119557302226No Hit
CATTGAGCCCACAATAACAGATGCGCAAACAGGTTCAGTAGTAGGCAGCT444740.4160019605614726No Hit
CTCCCTAGCATTGGGTGCCTGGACACATTGAGCCCACAATAACAGATGCG419100.3920187562875235No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAATGCTAGGGAGAGCT409660.3831887466016389No Hit
CATGAAGACAGTGTGGCAATTCCTCAAGCTTCTCCTTGGTGAAGGAATGA400540.37465805927798773No Hit
GTGTGGTATAGGCACAAAGATAGAAAATAAATCAGTGAAATAAAACAGAG354380.33148080852582334No Hit
ATGCTAGGGAGAGCTGCCTACAATGCTAGGGAGAGCTGCCTACTACTGAA345470.3231465515023878No Hit
AGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTGTTATTGTGGGC343550.32135061732898756No Hit
AGCATTGGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGCAAACA321490.30071608198252425No Hit
CCCTAGCATTGAGCCCACAATAACAGATGCGCAAACAGGTTCAGTAGTAG302800.2832337852633312No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGC301320.2818494193380018No Hit
CACCAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTGT297040.27784598274313044No Hit
CTCCCTAGCATTGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGC265630.24846562212516068No Hit
GACACATTGAGCCCACAATAACAGATGCGCAAACAGGTTCAGTAGTAGGC260060.24325554225753604No Hit
GTTCACCAATATTCCAGGCACCAATGCTAGGGAGAGCTGCCTACTACTGA244490.22869163857011837No Hit
ATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTGTTCACC227010.21234115453312025No Hit
CATTAGTAGCGCAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGC224860.21033008241186477No Hit
GCCTACAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCT218690.2045587731150525No Hit
GTCCAGACTTTAATAACAACATTAGTAGCGCAATGCTAGGGAGAGCTGCC211210.19756211289784736No Hit
ATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTT204740.1915101888864413No Hit
GCGCATCTGTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCC203480.1903316070851474No Hit
CTGTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTACTAC186920.17484167483957022No Hit
TTGGTAGACTTTAATAACAACATTAGTAGCGCAATGCTAGGGAGAGCTGC184540.1726154647704595No Hit
CACCCAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTG184520.1725967571228199No Hit
GTAGTAGGCAGCTCTCCCTAGCATTGGGTGCCTGGAATATTGGTGAACAG184350.17243774211788346No Hit
TTGGTAGACTTTAATAACAACATTAGTAGCGGAACAGATGCGCAAACAGG181540.16980931762452162No Hit
CCCTAGCATTGGTGCCTGGAATATTGGTGGGTGCCTGGAATATTGGTGAA178780.16722766225025876No Hit
GCGCAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTGT177960.16646064869703572No Hit
TCTGTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTACTA176580.1651698210099043No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCAATGCTAGGGAGAGCT175780.16442151510432088No Hit
GTGCCTGGACACATTGAGCCCACAATAACAGATGCGCAAACAGGTTCAGT175240.16391640861805204No Hit
CTACAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTGT173790.16256010416418207No Hit
TTGGTGAACAGATGCGCAAACAGGTTCAGTAGTAGGCAGCTCTCCCTAGC173140.16195210561589551No Hit
CTTCTGTGGTGGCACGTGGGTCATCAGTTGAGTTGTCCACACATGGGTGG172270.16113832294357353No Hit
CATCTGTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTAC171450.16037130939035052No Hit
ACCCAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTGT170160.1591646661175972No Hit
GCCTACTACTGAACCTGTTTGCGCATCTGTTATTGTGGGCTCAATGCTAG162040.15156936117592532No Hit
GTGTAGGGCTGAGCTGCAGGCTGGGTCTTCAGGTGGTGTCCCTGCACATC160590.15021305672205532No Hit
CTACTACTGAACCTGTTTGCGCATCTGTTATTGTGGGCTCAATGTGTCCA156820.14668666514199338No Hit
TTGCGCATCTGTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTG156530.1464154042512194No Hit
GCCTACTACTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACC155300.14526488392138487No Hit
GGACACATTGAGCCCACAATAACAGATGCGCAAACAGGTTCAGTAGTAGG154570.14458205478253996No Hit
GTGGTATAGGCACAAAGATAGAAAATAAATCAGTGAAATAAAACAGAGAA153050.14316027356193142No Hit
TTGGTGAACAGATGCGCAAACAGGTTCACAATGCTAGGGAGAGCTGCCTA151810.1420003994082771No Hit
CTTTAATAACAACATTAGTAGCGCAATGCTAGGGAGAGCTGCCTACTACT151340.14156076968874684No Hit
GCCCACAATAACAGATGCGCAAACAGGTTCAGTAGTAGGCAGCTCTCCCT150290.1405786181876686No Hit
GACTTTAATAACAACATTAGTAGCGCAATGCTAGGGAGAGCTGCCTACTA148440.13884816078100687No Hit
CACCTGGAATGTGTTTTTGTGAATGGTGTGGGACTGGGTTCCCGGGTTCG145830.1364068127640409No Hit
GTATAGGCACAAAGATAGAAAATAAATCAGTGAAATAAAACAGAGAACAG144770.13541530743914285No Hit
GGCTCAATGTGTCCAGACTTTAATAACAACATTAGTAGCGCAATGCTAGG143280.1340215876899937No Hit
CAATGTGTCCAGGCACCCAATGCTAGGGAGAGCTGCCTACTACTGAACCT142840.1336100194419228No Hit
GGTATAGGCACAAAGATAGAAAATAAATCAGTGAAATAAAACAGAGAACA140920.13181408526852256No Hit
CCAATATTCCAGGCACCAATGCTAGGGAGAGCTGCCTACAATGCTAGGGA139820.13078516464834533No Hit
GATAACCTACTGTCCCGTTCTTGTCTAAGGAGGAGACAGCCCTTGCCCCA139320.13031747345735567No Hit
GGTTCAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTG138700.12973753638052854No Hit
GCCATTGCCTGAGATAGGTAAAACAGAAGTAAACGCCCTGGCAGCCCCCA138240.12930726048481803No Hit
TGTGTGGACAACTCAACTGATGACCCACGTGCCACCACAGAAGAGTGGGG137740.1288395692938284No Hit
GGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTGTTATTGTGGGCTC137490.12860572369833356No Hit
CCCTAGCATTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTT137480.12859636987451378No Hit
AAATAAATCAGTGAAATAAAACAGAGAACAGCAAAACAGATCCACCCATG131710.12319921353049323No Hit
GTCCCGTTCTTGTCTAAGGAGGAGACAGCCCTTGCCCCACTCTTCTGTGG129150.12080463463262624No Hit
ATATTCCAGGCACCAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTT128410.12011245166996154No Hit
CAACATTAGTAGCGCAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTT127410.11917706928798225No Hit
CTATTATCTGCATTATAGTTAAAAACATCGACATGAACAAACAAAAGCCC125060.1169789206903309No Hit
GCCATTCACCTGGAATGTGTTTTTGTGAATGGTGTGGGACTGGGTTCCCG123840.11583775418431616No Hit
CCCTAGCATTGGCTGACCTCTAGCATACTCTGTCAAACTACAGGCAGCTG122910.11496784856907542No Hit
ATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTG120410.11262939261412717No Hit
CAATGTGTCCAGACTTTAATAACAACATTAGTAGCGCAATGCTAGGGAGA115330.10787765011367235No Hit
GTGTTTTTGTGAATGGTGTGGGACTGGGTTCCCGGGTTCGTTTCTAAAGA113760.10640909977396484No Hit
GTTCACAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCT113450.10611913123555128No Hit
AAATAAAACAGAGAACAGCAAAACAGATCCACCCATGTGTGGACAACTCA111810.10458510412910521No Hit
GCGCATCTGTTCACCAATATTCCAGGCACCAATGCTAGGGAGAGCTGCCT111610.10439802765270936No Hit
GGCACCCAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATC110610.10346264527073006No Hit
GAGCTGCCTACTACTGAACCTGTTTGCGCATCTGTTATTGTGGGCTCAAT109150.10209698699304029No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGAAG195800.0121.533131
ATGAAGA218150.0109.5315252
GACAGTG226100.0106.402917
AGACAGT248900.097.0548256
TGAAGAC282950.086.2513353
GAAGACA300200.080.2691654
CAGTGTG314050.075.226459
AAGACAG352850.073.83845
ACGAGGT15400.067.8285452
ACAGTGT388100.064.999928
GACGAGG16600.062.9535641
CGCCGTT9900.061.514145145
CGAGGTC17450.059.415813
GAAATTG45000.051.5583343
GTAGACT120350.051.4410934
GTCTGCC74100.050.9044652
TGAAATT46550.050.1772582
GGTAGAC123300.049.8648153
TCATAGC108000.049.72491
TGGTAGA129450.048.3032072
Produced by FastQC (version 0.11.5)