FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-51_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-51_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15054164
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC3791932.5188579053609352No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC3654322.427447980505593No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG2912321.9345610955214783No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC2635371.750592062103216No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT1457410.9681108828095669No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC1363280.9055833322926468No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG1255170.8337693145896379No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG1143800.7597897830792862No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT1072230.7122481195236082No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC1068760.7099431094280626No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC945140.6278262944391997No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA919910.6110668118136616No Hit
CTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGG850200.5647606868106393No Hit
ACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACG801530.5324307613494844No Hit
AACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCA717750.4767783850368576No Hit
ATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCC676590.4494371125490595No Hit
GTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCC670840.4456175713244521No Hit
TTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCG613810.40773436505673777No Hit
CCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGA612810.40707009701767566No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC605570.4022607964148657No Hit
GTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGGAACGC588530.3909416690292466No Hit
GTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAG578890.38453812513268754No Hit
AACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCA577120.3833623707035475No Hit
CTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTC576910.3832228744153445No Hit
CCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGA555720.3691470346676175No Hit
CTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCGA532500.35372273080059446No Hit
TTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACC522160.3468541992766918No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG521830.34663499082380134No Hit
GGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACC507390.33704296033974385No Hit
CTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATT494710.3286200416044358No Hit
ATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGA491950.3267866618166243No Hit
GATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAA461260.3064002756978069No Hit
GGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGG460110.30563636745288547No Hit
CTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATT455890.3028331563280432No Hit
CTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCT446140.2963565429471872No Hit
AAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAAT430710.2861068871044583No Hit
GGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGC420830.27954391887852426No Hit
GCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTC420640.27941770795110243No Hit
CACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTC420310.2791984994982119No Hit
GGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC412940.27430284405032385No Hit
CTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAA410060.2723897520978249No Hit
GCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATT409480.2720044766351688No Hit
GTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTG393930.26167510862775245No Hit
ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTC393580.2614426148140807No Hit
CCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTA392420.26067206388876857No Hit
CAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCA381520.25343154226299114No Hit
ACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCAT380800.2529532692748664No Hit
CATCTGGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAAT380800.2529532692748664No Hit
CCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCG379630.25217607566916367No Hit
CCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAG377010.25043569340682087No Hit
TCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGG372600.24750627135455677No Hit
CACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCA367470.24409857631416798No Hit
CCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGG366600.24352066312018386No Hit
TGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTG362630.24088351900510716No Hit
CAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAA351200.23329093531862677No Hit
ACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATA344970.2291525454352696No Hit
CCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTG344240.22866762976675423No Hit
GAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTT339420.22546585781847467No Hit
AAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCAC337130.22394468400902234No Hit
ATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTC335090.22258957720933556No Hit
GGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAG326670.21699644032043228No Hit
GATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAA318160.21134351930801337No Hit
CTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGT317530.21092503044340422No Hit
CCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATG314840.20913814941832704No Hit
CCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTC309960.20589652138770376No Hit
AATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAG308370.20484033520559494No Hit
GCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAG303330.20149242428872172No Hit
AATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGC303170.20138614140247177No Hit
ATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCA299810.19915420079122292No Hit
GTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCC299730.19910105934809796No Hit
CCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGA299430.1989017789363793No Hit
GATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGG281720.18713759196458868No Hit
AAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAA280610.18640025444122968No Hit
ATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTT272780.18119903569537305No Hit
CTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGAT272190.1808071175523264No Hit
GTTTTGATCGCGCCCCACTGCGTTCTCCATTCTGGTTACTGCCAGTTGAA267650.17779134065498423No Hit
GAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGT258040.17140772479959698No Hit
GAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACG250050.1661002231674904No Hit
GAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAA245330.16296487802311704No Hit
GAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTGGGGC243520.1617625528724146No Hit
CAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAAT242190.1608790763804619No Hit
GCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAAC241520.16043401679429029No Hit
ACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGG240250.15959039638468134No Hit
TCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAAT234640.1558638526855427No Hit
GCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGG233600.15517301392491803No Hit
GAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTC230690.1532399939312472No Hit
GTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTGG228550.1518184603276542No Hit
CTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTC228530.15180517496687296No Hit
CTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGC227240.15094826919648277No Hit
GACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGCT226480.15044342548679557No Hit
CTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGACCAA215680.14326933066492434No Hit
CGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGC215290.14301026612969012No Hit
GTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGT213980.1421400749985187No Hit
ATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAAT206850.13740384388000554No Hit
GGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCA204870.1360885931626625No Hit
GCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCA204220.1356568189372721No Hit
TCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCG202560.13455413399242894No Hit
TCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCC199790.13271411152422677No Hit
GCGTTCTCCATTCTGGTTACTGCCAGTTGAATCTGAGGGTCCACCAAACG199550.13255468719485186No Hit
ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTA199450.13248826039094566No Hit
GTCTGGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTG199410.13246168966938315No Hit
AATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTT199020.13220262513414893No Hit
ACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGT195900.13013010885227502No Hit
ACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGAT195610.129937471120947No Hit
GGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCG193470.128515937517354No Hit
GTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACT192560.12791145360180745No Hit
CCACTGCGTTCTCCATTCTGGTTACTGCCAGTTGAATCTGAGGGTCCACC190660.12664934432758937No Hit
GAATTCGTCTGGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTG189730.12603157505126159No Hit
ACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAA189410.12581900927876166No Hit
ACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTA185880.1234741431008723No Hit
TAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACG185860.12346085774009104No Hit
CCAAGACGCAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATC183430.12184668640517003No Hit
TGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACA182330.12111599156220167No Hit
ATTCTGGTTACTGCCAGTTGAATCTGAGGGTCCACCAAACGTAATGCGGG181360.12047165156431137No Hit
CTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTG180090.11962803115470244No Hit
CCAAAACGCAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATC179340.11912983012540583No Hit
AGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGT178810.11877776806470289No Hit
GGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGACCAAATTGG177630.11799393177860956No Hit
GATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGG177620.11798728909821893No Hit
ACGTAATGCGGGGTGCATTTCGCTGATTTTGGGGTCCATTATCAGACATT173200.11505122436556424No Hit
CTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGT172700.11471909034603317No Hit
TTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCG170400.11319127385619022No Hit
ATCTACAACAGTAGCAACAATGGAAGTAACTTCAGGCTCAGGAACAACTG169400.11252700581712807No Hit
ATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAAC168840.11215501571525327No Hit
ACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCT164760.1094448021158797No Hit
CAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAA164560.10931194850806727No Hit
ACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTGGTTACTGCCAG163940.10890010232384874No Hit
TTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAA162530.10796348438877111No Hit
TCTGGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGG162220.10775756129666184No Hit
GGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGT161570.10732578707127144No Hit
ACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCCAATTT160500.10661502026947495No Hit
ATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTT159470.10593082418924092No Hit
CATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGC159430.10590425346767844No Hit
GGTTACTGCCAGTTGAATCTGAGGGTCCACCAAACGTAATGCGGGGTGCA158520.10529976955213188No Hit
TTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGA156730.10411072976221063No Hit
GATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGG155400.10322725327025799No Hit
ATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGT154530.10264934007627391No Hit
TCTGGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTT152950.10159979657455571No Hit
CCATTCTGGTTACTGCCAGTTGAATCTGAGGGTCCACCAAACGTAATGCG150950.10027126049643141No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA566750.0140.133091
TCCTAGG661700.0119.975123
TTCCTAG669500.0118.586832
CCTAGGT683550.0115.98834
CTAGGTA712050.0111.736595
TAGGTAG736350.0108.51116
AGGTAGT888700.089.867847
CTTGTAG887450.070.112531
GGACCGA12800.064.065551
ATCTGTT1054300.059.4432758
TTGTAGA1062600.058.7629662
TCTGTTC1094850.057.1845369
ACCGAGA14250.056.9876863
AGATCTG1129300.055.7824976
GACCGAG15550.052.7131962
GATCTGT1199100.052.3645637
GTAGAGC68200.049.4363563
TGTAGAG71350.047.173192
GTCCGAT3850.047.123271
TTTCGCG7450.046.709957145
Produced by FastQC (version 0.11.5)