FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-50_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-50_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9938531
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGCATTGGTGCCTGGAATATTGGTGAACGCTACTAATGTTGTTATT2152142.165450809581416No Hit
CCCTAGCATTGTAGGCAGCTCTCTCTCTCGCTACTAATGTTGTTATTAAA1110641.1175092174084882No Hit
GCCTATGCTAGGGAGAGCTGCCTAGACTTTAATAACAACATTAGTAGCGT1048661.0551458761863297No Hit
CCCTAGCATTGCGCTACTAATGTTGTTATTAAAGTCTAGGCAGCTCTCCC969670.9756673295077511No Hit
CTACTAATGTTGTTATTAAAGTCTAGGCAGCTCTCCCTAGCATAGGCAGC720080.7245336358059355No Hit
GAATATTGGTGAACGCTACTAATGTTGTTATTAAAGTCTAGGCAGCTCTC690380.6946499437391703No Hit
CTCCCTAGCATTGGTGCCTGGAATATTGGTGAACGCTACTAATGTTGTTA654830.6588800699016786No Hit
GCCTAAGACTTTAATAACAACATTAGTAGCGAGAGAGAGAGCTGCCTACA649900.6539195782555792No Hit
CTCCCTAGCATTGTAGGCAGCTCTCTCTCTCGCTACTAATGTTGTTATTA616950.6207657852050771No Hit
ATGCTAGGGAGAGCTGCCTATGCTAGGGAGAGCTGCCTAGACTTTAATAA616530.6203431875394865No Hit
TTGGTAGACTTTAATAACAACATTAGTAGCGAGGGAGAGCTGCCTAGACT581400.5849959113675854No Hit
CTCCCTAGCATTGCGCTACTAATGTTGTTATTAAAGTCTAGGCAGCTCTC512810.5159816878369651No Hit
GCATTGGTGCCTGGAATATTGGTGAACGCTACTAATGTTGTTATTAAAGT492150.4951939074295789No Hit
ATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTT484940.4879393141702732No Hit
ATGCTAGGGAGAGCTGCCTAAGACTTTAATAACAACATTAGTAGCGAGAG449600.4523807391655769No Hit
CCCAATGCTAGGGAGAGCTGCCTATGCTAGGGAGAGCTGCCTAGACTTTA398720.40118605053402767No Hit
ATTGTAGGCAGCTCTCTCTCTCGCTACTAATGTTGTTATTAAAGTCTTAG374500.3768162518183019No Hit
CCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTAAGACTTTAATA373960.3762729119625426No Hit
ATGCTAGGGAGAGCTGCCTAGACTTTAATAACAACATTAGTAGCGTTCAC369460.37174507983121446No Hit
TTGGTAAGACTTTAATAACAACATTAGTAGCGAGGGAGAGCTGCCTAGAC361450.3636855386374505No Hit
GTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTT335090.33716250419704885No Hit
GGTAAGACTTTAATAACAACATTAGTAGCGAGGGAGAGCTGCCTAGACTT325650.32766411857044064No Hit
GTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTAGA325630.3276439948720792No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG307850.309754027028743No Hit
CCCAATGCTAGGGAGAGCTGCCTAAGACTTTAATAACAACATTAGTAGCG306640.3085365432778748No Hit
GCCTAGACTTTAATAACAACATTAGTAGCGTTCACCAATATTCCAGGCAC304840.30672541042534357No Hit
GGGTAGTAGAGTTGAATAGAATGGAATGCAATGAAATGGAACGAAGTGGA303100.3049746486678967No Hit
GCATTGTAGGCAGCTCTCTCTCTCGCTACTAATGTTGTTATTAAAGTCTT300450.30230825863500355No Hit
CACCAATGCTAGGGAGAGCTGCCTATGCTAGGGAGAGCTGCCTAGACTTT280010.28174183890959337No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC273460.27515132769621586No Hit
GCCTACAATGCTAGGGAGAGCTGCCTAGACTTTAATAACAACATTAGTAG260330.26194011972191866No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG253530.25509806227902293No Hit
ACTAATGTTGTTATTAAAGTCTAGGCAGCTCTCCCTCGCTACTAATGTTG246520.2480447060033319No Hit
CTACTAATGTTGTTATTAAAGTCTAGGCAGCTCTCCCTCGCTACTAATGT243020.2445230587900767No Hit
GTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTAAGACTT236760.23822434120294037No Hit
AATTAAATGTTGGTGATTATTTTGTGCTGACATCACATACAGTAATGCCA234270.23571894075693883No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC229180.23059745952394775No Hit
CCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTATGCTAGGGAGA225130.22652241060575248No Hit
ATACTAAGCTTGTCCTGTTTATTCTTAACAAAAGCTAGGTTCTCTGCAAG216620.2179597769529521No Hit
GTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACATCA215150.21648068512338495No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGC213490.2148104181593839No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG202310.20356127077532887No Hit
CATTAGTAGCGAGGGAGAGCTGCCTAGACTTTAATAACAACATTAGTAGC201930.20317892050646116No Hit
CTACTAATGTTGTTATTAAAGTCTAGGCAGCTCTCCCTAGCATTGTAGGC201690.20293743612612367No Hit
GTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTA193830.19502882267007066No Hit
AGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACAT189910.19108457779122487No Hit
GTCTCAAGGGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGT187670.1888307235747416No Hit
ATATTGGTGAACGCTACTAATGTTGTTATTAAAGTCTAGGCAGCTCTCCC187460.18861942474194626No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT187120.18827732186980148No Hit
GCTAGGGAGAGCTGCCTATGCTAGGGAGAGCTGCCTAGACTTTAATAACA183050.18418214925324478No Hit
TCATAGCACATTGGTAAGACTTTAATAACAACATTAGTAGCGAGGGAGAG179080.18018759512849536No Hit
TCATAGCACATTGGTAGACTTTAATAACAACATTAGTAGCGAGGGAGAGC177840.17893992583008494No Hit
ATCCTAAACTATACATTGTCATTCTATTCCACAAATCCTTGCAGAGAACC176610.1777023183808553No Hit
GACTTTAATAACAACATTAGTAGCGTTCACCAATATTCCAGGCACCAATG175610.17669613346278237No Hit
GTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTATGCTAG175560.17664582421687872No Hit
GCATTGGGTGCCTGGAATATTGGTGAACGCTACTAATGTTGTTATTAAAG173690.1747642584200824No Hit
CCAATGCTAGGGAGAGCTGCCTATGCTAGGGAGAGCTGCCTAGACTTTAA172360.17342603247904542No Hit
GTTGAATAGAATGGAATGCAATGAAATGGAACGAAGTGGAATCGAGTGGA167710.16874727261000644No Hit
GTATTTGTACATGGTGCAGAGGGAAAAAGTGGTTAAAATGGAAGGGATGT166930.16796244837390958No Hit
CACCAATGCTAGGGAGAGCTGCCTAAGACTTTAATAACAACATTAGTAGC164810.16582933634759503No Hit
ATTCAATTCTGTTCTACTCGACTCCAATACATTCAATTCCATTCCATCCG162960.16396789424916017No Hit
GTCTATGGGTGTGGGGCATGGTATTTGTACATGGTGCAGAGGGAAAAAGT162660.16366603877373828No Hit
GCCTAGACTTTAATAAAATACAATGCTAGGGAGAGCTGCCTAGACTTTAA160690.16168385448513467No Hit
GCTCTCTCTCTCGCTACTAATGTTGTTATTAAAGTCTTAGGCAGCTCTCC154000.15495247738322696No Hit
CAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTT153720.15467074560616656No Hit
ATTCCATTCCATTCGATGTCTTTCCATAACGATCCATTCCATTCTATTCC153010.1539563543143348No Hit
GAATAGAATGGAATGCAATGAAATGGAACGAAGTGGAATCGAGTGGAATG147740.14865375979609058No Hit
GGGTTATACAGAGACCCTAATGAAAACAATTGGACTAGAGCGATGAAGGG146990.1478991211075359No Hit
CTACTAATGTTGTTATTAAAGTCTTAGGCAGCTCTCCCTAGCATTGGGTG146580.14748658529112602No Hit
CTCAAGGGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTG142230.1431096808975089No Hit
TCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAA141380.14225442371714692No Hit
GGTAGACTTTAATAACAACATTAGTAGCGAGGGAGAGCTGCCTAGACTTT141060.1419324445433636No Hit
GCTCTCCCTAGCATTGGTGCCTGGAATATTGGTGAACGCTACTAATGTTG136640.13748510720548138No Hit
GCTAGGGAGAGCTGCCTAAGACTTTAATAACAACATTAGTAGCGAGAGAG134530.13536205702834755No Hit
ATTCCATTCCATCCGATTCCATTACATTCTTTTAATTTCTTATCCATTTC133970.1347985934742267No Hit
TGTCTATACTAAGCTTGTCCTGTTTATTCTTAACAAAAGCTAGGTTCTCT133580.1344061813561783No Hit
GGTAGCAGACTTTAATAACAACATTAGTAGCGAGGGAGAGCTGCCTAGAC132730.13355092417581632No Hit
ATTCTATTCCTTTCGATTCCATTCCATTCTATTTCATTCCATTCGATTCC132330.13314845020858715No Hit
GCGCATCTGTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCC131890.1327057288446351No Hit
CTCCCTAGCATTGTATTTTATTAAAGTCTAGGCAGCTCTCCCTAGCATTG130060.13086441044456168No Hit
ATTCTGTTCTACTCGACTCCAATACATTCAATTCCATTCCATCCGATTCC128190.12898284464776535No Hit
CCCTAGCATTGCTCAATTAGAATGGGAGAAATGGAGAATGGTTGACAGTG127020.12780560829362006No Hit
ACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACT124360.12512915641154612No Hit
CCTTTCCATTCGAGTGCATTCCATTCCGTTCCATTTCTTTCCGTTCTGTA122250.12300610623441231No Hit
GAATGGAATGCACTCGAATGGAAAGGAGTCCAATGGAAGGGAATCGAATG121770.1225231374737373No Hit
CTGCCATTCCATTCAATTCTGTTCTACTCGACTCCAATACATTCAATTCC121060.12180874618190556No Hit
GTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCA119560.12029946880479621No Hit
GCATTGCGCTACTAATGTTGTTATTAAAGTCTAGGCAGCTCTCCCTCGCT117910.11863926368997592No Hit
GGCTTATACCCAACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGC116940.11766326431944521No Hit
GTCTTAGGCAGCTCTCCCTAGCATTGGGTGCCTGGAATATTGGTGAACAG116680.11740165624074624No Hit
GGTTCACAATGCTAGGGAGAGCTGCCTATGCTAGGGAGAGCTGCCTAGAC116250.11696899672597488No Hit
GCGTTCACCAATATTCCAGGCACCAATGCTAGGGAGAGCTGCCTATGCTA115950.11666714125055301No Hit
TAGCATTGGTGCCTGGAATATTGGTGAACGCTACTAATGTTGTTATTAAA115230.11594268810954053No Hit
TTGCGCATCTGTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTG112560.11325617437828589No Hit
CCTTTTGATAATTTGCAACATTGCTAGAAAACTCATCTGAGATATTGAGT112090.11278326746679163No Hit
GAATGAAATAGAATGGAATGGAATCGAAAGGAATAGAATGGAATGGATCG110490.11117337159787498No Hit
GAATAGAATGGAATGGATCGTTATGGAAAGACATCGAATGGAATGGAATT110280.11096207276507968No Hit
CATTAGTAGCGTTCACCAATATTCCAGGCACCAATGCTAGGGAGAGCTGC109830.11050928955194687No Hit
GTAGTAGAGTTGAATAGAATGGAATGCAATGAAATGGAACGAAGTGGAAT108450.10912075436500625No Hit
TTGGTAGACTTTAATAACAACATTAGTAGCGCAATGCTAGGGAGAGCTGC107940.10860760005678909No Hit
GAGTTGAATAGAATGGAATGCAATGAAATGGAACGAAGTGGAATCGAGTG107580.10824537348628283No Hit
ATTCCATTCTATTCCTTTCGATTCCATTCCATTCTATTTCATTCCATTCG107200.10786302321741513No Hit
GAATGGAATGCAATGAAATGGAACGAAGTGGAATCGAGTGGAATGGAATC106450.10710838452886046No Hit
CTATAAAACAAGCATAATCATGAAGATGAATGGAGGAAGTCAGGCTATGT106090.1067461579583542No Hit
ATAGAATGGAATGCAATGAAATGGAACGAAGTGGAATCGAGTGGAATGGA105110.10576009673864276No Hit
CCCTAGCATTGGGTGCCTGGAATATTGGTGAACGCTACTAATGTTGTTAT104850.10549848865994381No Hit
GTATAACCCTTTCAGTTTTTCAGAAAAGGCACTGTTGGGACTGGCAAATC104530.10517650948616047No Hit
GTAGGCAGCTCTCTCTCTCGCTACTAATGTTGTTATTAAAGTCTTAGGCA103910.10455267483695527No Hit
GAATCGTACAGAACGGAAAGAAATGGAACGGAATGGAATGCACTCGAATG103780.1044218707976058No Hit
CACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCAC102500.10313395410247249No Hit
GTTCTACTCGACTCCAATACATTCAATTCCATTCCATCCGATTCCATTAC101250.10187622295488137No Hit
CCCTAGCATTGTATTTTATTAAAGTCTAGGCAGCTCTCCCTAGCATTGGT101180.10180579001061625No Hit
GAGATATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTG101150.10177560446307406No Hit
GTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTG100150.10076941954500115No Hit
GAAGGGTAGTAGAGTTGAATAGAATGGAATGCAATGAAATGGAACGAAGT99590.10020595599088034No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGTA36950.0117.992572
GAAGGGT37250.0116.703341
AGGGTAG40000.0109.304963
GGGTAGT126250.0101.230351
GTCTCAA32400.096.76571
GGTAGTA138150.092.628012
TATGGGT33200.089.30734
CTATGGG34200.087.3481453
TCTCAAG36350.086.213242
TCTATGG38850.082.159222
GGTAGCA39350.079.490511
TGGGTGT38000.077.842656
GTAGCAG40150.076.427472
CTCCTAG9650.075.958571
TAGCAGA40300.075.925913
AGCAGAC42050.072.4077454
TCAATGT100400.071.9163064
ATTGCTC30600.068.245058
GGGTGTG44100.067.245467
ATGGGTG45800.064.262865
Produced by FastQC (version 0.11.5)