FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-50_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-50_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4138605
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGGAGGCCAGCTCCCTGGGCTGGAGAACTGCCTCTGTTGTCCAG243910.5893531757681634No Hit
GGTATATGCAGGCAGAATCATGGACTCATGGAAAGGCTCCTCCATAGGGG232980.5629433106082847No Hit
AAATAGGAGTGGTCCCCAGAGGACAAGGGAAGAAGAAAGTGTGTTTCAAG104830.2532979107694501No Hit
GTGCAGGTATATGCAGGCAGAATCATGGACTCATGGAAAGGCTCCTCCAT97070.23454763138787105No Hit
CCCCTATGGAAGAGCCTTTCCATGATTCCCTGAATCCTCAGACCCCCTGA95930.23179308003542257No Hit
ACATTATTATCACCATTACCATCATTGTTTGACATCATCATTGTCATCAT81240.19629802795869622No Hit
GTATGCAACCAACCACTCTGCAAACTGAACAGTGGCAGGTTCTCACTATC66630.16099627773126451No Hit
GTTCCTAGGTAGTAGAAATGAAGGTCAAAAATGTGAACACGCTGAATCTG63930.15447234031757076No Hit
GTTCCTAGGTAGTAGAAATCGTAGGATGCCTCAGTGAAGAAGTCAGGGAT62570.15118620887956208No Hit
GGTATATGCAGCAGGAGAATCAAGGAATACAATAGAGTGTAGAGGAGTCA61860.14947065496707224No Hit
GTCAAACAAACCCCTTTTCTTTATAAATTATCCAGCCTCTGGTATTCCCT60920.14719935823786034No Hit
GTCCTGTAGCAACCAGGGATTAGTATCTGCTTGATTACTTTGTTTATTGA59100.1428017411664075No Hit
GTTCCTAGGTAGTAGAATCAAATAGGAGTGGTCCCCAGAGGACAAGGGAA58280.14082039721113757No Hit
CAAGAAAGACGTGGGACCCTGAGACTTGGGAGACAGACAAGTCAGATAGG57170.1381383340521746No Hit
AGCCTGCACAGTCAAACAAACCCCTTTTCTTTATAAATTATCCAGCCTCT56960.13763091669777616No Hit
TTCCTAGGGTGTGTCATCACCTGCAGGTGGGTGGGCAAGGGGGCTTGCCT54620.13197683760590828No Hit
CCCAAAGACAGTGCAAAAGATGGAATACAGAAATTTTTTTTCTCTGGTCT52020.1256945275038328No Hit
GTGTTGGAAAAGGCTGTGTGATGACAGGTGAAGATACCCAGTGGGCAGGC51590.12465553006387418No Hit
ATCATGGACTCATGGAAAGGCTCCTCCATAGGGGAGGCCAGCTCCCTGGG49910.12059619122868698No Hit
TTTTTATTGTGTCCAAATTAACAATGGTGTACATTTACAAACCAGCAGCT46820.11312990729968189No Hit
CTGATAGACTGAGCAAGATGAAGCCTGGGATTTGACCATTGTATCTGCAA43760.10573611156416232No Hit
TCATTGCACTGCCTCTTTTAATTATTCCTATGACAGATAAAAGCCTTCTC43560.10525285694092575No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA106000.0117.4627151
TAGGTAG125550.098.919926
CCTAGGT145500.085.3535844
CTAGGTA145700.085.130735
TCCTAGG147300.084.3299263
TTGTAGA40850.080.86511
TTCCTAG156900.078.93772
TATATGC66250.078.699163
AGGTATA28800.078.282345
AGGTAGT167900.074.229887
ATTAGCA29000.070.848431
GGTAGTA177400.068.051938
GTATATG78100.066.610392
GGTATAT79050.066.401541
ATATGCA79000.065.799024
CAGGTAT36850.061.775964
TTAGCAA34300.059.864062
TATGCAG89300.057.393655
GTAGTAG192600.056.6208849
TGTAGAG65900.055.600642
Produced by FastQC (version 0.11.5)