FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-48_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-48_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10074884
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCAAAAGCTGCATATGATGGAAGGGAACTAAACTCTGAGGCTATAGC964020.956854689344314No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT521340.5174650149818102No Hit
CCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGT475440.4719061777783248No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC378910.3760936602346985No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT376470.37367179612192064No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC359530.3568577067487824No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA337910.33539840260195547No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA328990.3265447026486856No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC326390.3239640277744141No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT303820.30156178473121875No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA303550.30129379157119823No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG300100.29786943452649184No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC294380.2921919498030945No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA278230.2761619885648311No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG260640.2587027304731251No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG247010.24517403872838633No Hit
GTTGTAAGAGGTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTT245490.24366533649419686No Hit
GTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGA241000.23920870949978185No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC240420.23863302048936744No Hit
GGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTA229120.22741701045887971No Hit
CTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCC228400.2267023620321584No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG213350.21176422477916368No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG210880.20931258364860578No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG210450.20888577972709163No Hit
CAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGA208280.20673190877433428No Hit
CTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACT208270.20672198310174092No Hit
GAACAATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAA207990.20644406426912706No Hit
CCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCT206500.20496513905271763No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT204980.20345643681852812No Hit
TCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCTATTGAAACAC199060.1975804386432638No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT198760.19728266846546322No Hit
CATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGTTA192090.1906622448456975No Hit
CTCCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAG190270.1888557724337074No Hit
ATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTT189990.18857785360109358No Hit
GTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTT189880.1884686712025667No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT188530.18712870540246418No Hit
GCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTAC185670.18428996304076553No Hit
GTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTTGATAATGTTG183520.18215594343319486No Hit
GTCCAACATTTTGCTTCAGACATAAAAACATTGTTTTGATAATAAAGAAC183290.18192765296354776No Hit
CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTT178410.177083924737992No Hit
GACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTG177920.17659756678091776No Hit
GCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTA177150.17583328999122969No Hit
GTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTC174570.17327246646214486No Hit
TTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGAATCACCATTAGC174450.17315335839102464No Hit
GTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTT173620.17232952756577644No Hit
GTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTCTCAGT169930.16866695437882956No Hit
CCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAAC169540.16827985314768884No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT166650.16541133376821013No Hit
ATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGAAAAGCAT162180.16097455811898181No Hit
CATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGT160060.1588703155291912No Hit
GTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAAC159700.15851299131583052No Hit
GACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCT159550.15836410622693026No Hit
GTACTTTTCTGTGTCTTTGATTTCGAGCAACATAAGCCCGTTAATACAAA159300.15811596441209647No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC156740.15557499222819834No Hit
GCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGT155630.1544732425703363No Hit
CATAAAAACATTGTTTTGATAATAAAGAACTGACTTAAAGTTCTTTATGC147450.1463540423889744No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG146990.14589746144968022No Hit
GAGTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACTCATTCA145770.14468652939329127No Hit
GAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCAT145070.14399173231175663No Hit
GAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGC144350.1432770838850353No Hit
GTAGATAAAAGTGCATTAACATTGGCCGTGACAGCTTGACAAATGTTAAA143380.14231429364348017No Hit
GTTCAAGGGAACACAACCATCTCTTGCATTGTTGATAATGTTGTTGAGTG142040.14098425351597102No Hit
ATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTG141880.14082544275447736No Hit
CTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATA141780.14072618602854386No Hit
CTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTATACATTTG141250.1402001253810962No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC141030.13998176058404246No Hit
GACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTC137710.1366864372830496No Hit
GTCTCAAGGGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGT137060.13604126856448173No Hit
GCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGT135770.13476085679993932No Hit
GTCCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAA134260.13326208023834318No Hit
CATAAGCTTCTTGAGCAGTAGCAAAAGCTGCATATGATGGAAGGGAACTA133960.13296431006054263No Hit
CATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGTTGCATGG132040.13105858092261904No Hit
GCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTC131610.13063177700110493No Hit
GTGAAAAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTC131310.13033400682330437No Hit
CACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGT130980.13000645962772375No Hit
GTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACTCATTCACA130860.12988735155660353No Hit
GTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAA129770.12880545324392817No Hit
ATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTAT129420.12845805470316085No Hit
CATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGA128310.1273563050452988No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT128300.12734637937270543No Hit
CAACAGCATCGTCAGAGAGTATCATCATTGAGAAATGTTTACGCAAATAT125360.12442823163025996No Hit
CTTTCTGTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCA125110.12418008981542616No Hit
ACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCGTCAGAGA123130.12221480664194248No Hit
GCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAA123100.12218502962416242No Hit
ACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATC122480.12156963792337461No Hit
ATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTT122180.12127186774557404No Hit
CACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAAT121560.12065647604478623No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA121330.12042818557513914No Hit
TAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCT120610.11971353714841779No Hit
GCATAAGTGCTATTGAAACACACAACAGCATCGTCAGAGAGTATCATCAT119870.11897903737650975No Hit
ACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGC118990.11810557818829479No Hit
GTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTT118830.11794676742680114No Hit
CTCAAGGGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTG117710.11683509209634572No Hit
TTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACAC117520.11664650431707205No Hit
TGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGA115110.11425441722207422No Hit
GTCAGAGAGTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACT113780.1129343027671584No Hit
GTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTAGA113780.1129343027671584No Hit
ACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGTAGACATAAACAAGCTTT112920.11208069492413014No Hit
CTATTAGCATAAGCAGTTGTGGCATCTCCTGATGAGGTTCCACCTGGTTT112800.11196158685300991No Hit
GTCTCAGTCCAACATTTTGCTTCAGACATAAAAACATTGTTTTGATAATA112390.1115546342766825No Hit
GTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGAAAAGCATTG112200.11136604649740882No Hit
CAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTT112120.11128664111666199No Hit
CTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAG111000.11017496578620657No Hit
AGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAACATTGT110110.10929158092539824No Hit
GTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCC109030.10821960828531624No Hit
GGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAACATTGTTTTGA108210.10740570313266139No Hit
GCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAAC108020.10721711535338768No Hit
CTCTTGCATTGTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTA106350.1055595280302979No Hit
TCATTATCCAACTTTCTAAGCATAGTGAAAAGCATTGTCTGCATAGCACT105420.10463644047911619No Hit
CTATAGAGACACTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATC105090.10430889328353558No Hit
CTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAA104680.10390194070720815No Hit
GCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGA104300.10352476514866077No Hit
GCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATA103820.10304833286417987No Hit
CTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCC103780.10300863017380646No Hit
GTGCTATTGAAACACACAACAGCATCGTCAGAGAGTATCATCATTGAGAA103730.10295900181083971No Hit
CCATTAGCAACAGCCTGCTCATAAGCTTCTTGAGCAGTAGCAAAAGCTGC102680.10191680618853774No Hit
TGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACAT102550.10178777244482418No Hit
ATTTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGAC102180.10142052255887016No Hit
GTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGT102150.1013907455410901No Hit
CTCTATTTCTATAGAGACACTCATAAAGTCTGTGTTGTAAATTGCGGACA101830.10107312401810284No Hit
GAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTG101390.10063639442399536No Hit
GTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAA101210.10045773231731502No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA1050.0117.531291
TCCCTAG40750.0110.74872
GCTCAAT37050.0106.141932
TCAATGT38500.0103.189014
GTCTCAA22350.0102.961681
CCCTAGC132050.0100.4920351
GGGTAGT48750.098.577361
CCTAGCA137700.097.058052
AAGGGTA14900.095.443352
AGGGTAG15050.094.913843
GCCGACC2100.086.30809145
GAAGGGT17050.083.4498441
TAGCATT169600.079.121444
TGCGCAT7750.072.963644
GGTAGTA68050.071.43692
GGTGAAC38700.066.704658
GCGCATC8600.064.90725
TAGAGCC58550.063.395244
ATAGAGC59300.062.2390443
TCGTGCG350.003563959862.1581849
Produced by FastQC (version 0.11.5)