FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-48_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-48_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12532161
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA1455321.161268196283147No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC761700.6077962132787793No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT633260.5053079034014963No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT596420.47591153672538994No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC560010.4468582872499005No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG553970.4420386875017006No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC516860.41242687514148596No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC505840.4036334994419558No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT501110.3998592102351701No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT496010.3957896806464583No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC473910.37815505242870723No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA384920.30714575084057727No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG374120.2985279234762464No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC359030.2864869035755286No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG328430.26206972604325784No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA325430.25967588510872147No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC320160.2554707045337193No Hit
TCATTATGCCTTTAGGTAATGTTGCACTATCACCATAATTTTGAAGGTCA320130.25544676612437395No Hit
GTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAA317290.2531805967063462No Hit
TTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAG315210.2515208669917343No Hit
TTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGC314070.2506112074366105No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA302140.2410916999869376No Hit
GTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAA298070.23784405578574996No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC291610.232689318306715No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT278470.22220429501344582No Hit
GTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTG272280.21726500321851916No Hit
GCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGAC271900.21696178336681118No Hit
AGGTAATGTTGCACTATCACCATAATTTTGAAGGTCACACTTTTCTAATA269200.21480732652572848No Hit
GAATTAGCTATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGC267530.21347475507216992No Hit
GCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTC251030.20030862993222No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC250180.19963037500076802No Hit
CTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACAC245990.19628697716219892No Hit
ATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACA240990.192297242271305No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG230040.18355972286024733No Hit
TTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGT227190.1812855739724378No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG226930.1810781077581113No Hit
GTAATAAATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCA222810.17779056620801473No Hit
CCTTTAGGTAATGTTGCACTATCACCATAATTTTGAAGGTCACACTTTTC209180.16691454889543791No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA205750.16417759076028468No Hit
ATGTTGCACTATCACCATAATTTTGAAGGTCACACTTTTCTAATAGCATT205190.16373074045250455No Hit
CATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAA201480.16077035716346127No Hit
GTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTAT200020.15960535457532024No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG196320.15665295075605873No Hit
CCATAATTTTGAAGGTCACACTTTTCTAATAGCATTCTTTGCATTTTGTA186510.14882509090012488No Hit
TATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGG185660.14814683596867292No Hit
ATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAG184430.14716536118551302No Hit
TATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATT183850.14670255193816933No Hit
ACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACG183720.1465988188310061No Hit
GTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACCGGG182810.14587268708086337No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG180860.14431669047341478No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA177300.1414759992310983No Hit
CCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTGGT176360.14072592907161025No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC175720.1402152430055758No Hit
ATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAG174700.13940133708783345No Hit
CAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCG172610.13773362790343982No Hit
ATTCATCATTATGCCTTTAGGTAATGTTGCACTATCACCATAATTTTGAA171040.13648085114769912No Hit
GGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATT168820.13470940885614222No Hit
CTATTGACTATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCAT167910.13398327710599953No Hit
GAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTTTAGCTAGT167070.13331300164432935No Hit
GGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTT164100.13094309911913835No Hit
GTTATGAAATAGTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAA163090.13013717267117778No Hit
GTCATTAAGATCTGAATCGACAAGCAGCGTACCCGTAGGCAACCACTGTC162660.1297940554705609No Hit
GCTATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGA161750.12906792372041823No Hit
TCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAG161480.12885247803630992No Hit
GTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATAT160900.12838966878896624No Hit
GTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCC160510.12807846946747653No Hit
GTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTT159390.1271847688519163No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC159030.12689750793977192No Hit
CATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCT158730.1266581238463183No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA158330.12633894505504678No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC157220.12545322390926833No Hit
TGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCACT156600.12495849678279748No Hit
ATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTAT155520.1240967140463644No Hit
CTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTG155050.12372167896662037No Hit
GATGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATAT154350.1231631160818952No Hit
TGACTATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAG153170.12222153864764425No Hit
ATTACAATCTAGTCAAGCGTGGCAACCGGGTGTTGCTATGCCTAATCTTT152790.12191831879593631No Hit
CATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTT149970.11966810831747214No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC149230.11907762755361984No Hit
GGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGG147830.11796050178416953No Hit
GTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGGAACGC146800.11713861639664541No Hit
CCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGA145890.11641248464650271No Hit
CCATAGGGAAGTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCA144780.11552676350072424No Hit
GACTATTGACTATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCC144740.1154948456215971No Hit
ATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCA144700.11546292774246994No Hit
GGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC142640.11381915696742166No Hit
TCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTAT141740.11310100468706076No Hit
CTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTC140970.11248658551386309No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC140830.11237487293691807No Hit
GAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTT140140.1118242895219747No Hit
ATCTAGTCAAGCGTGGCAACCGGGTGTTGCTATGCCTAATCTTTACAAAA139470.11128966504659493No Hit
ACGTAATGCGGGGTGCATTTCGCTGATTTTGGGGTCCATTATCAGACATT135880.10842503539493309No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT135650.10824150758995198No Hit
AATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCAT134860.10761112947719072No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG134720.10749941690024568No Hit
ATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCC134570.10737972485351888No Hit
CTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAG132090.1054008163476355No Hit
GGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACC132040.10536091899872656No Hit
TCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTA132040.10536091899872656No Hit
ACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAAT131600.1050098223283279No Hit
AACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCA131470.10490608922116465No Hit
GGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACG129630.10343786678131568No Hit
GCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGA128450.10249628934706473No Hit
AACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTGGTTTACAT128440.10248830987728294No Hit
GCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTC127720.10191378805299423No Hit
ACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTG127010.10134724569848727No Hit
AACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCA125430.10008648947296479No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAATG60900.082.675442
CAGGTAT20400.082.449974
TGGAAAT62100.081.2641147
GCAGGTA21600.079.2268453
AATGGAA75850.066.997515
TCGTGGG9100.062.937775145
CTCGTGG22350.061.0608331
TATGCGC34950.059.5293275
CTTAACC4150.057.689952
ATGCGCT36800.056.541656
GAAATTG90650.055.91759
AAATTAG421500.055.8961685
TGCGCTA37250.055.8646167
ATATGCG37400.055.8281824
CGCTAGT37350.055.5282259
GCCGACC4650.054.568348145
CAAATGG93600.054.152123
GTGCAGG32100.054.047461
GTATATG39250.053.7882772
TGCAGGT32450.052.986832
Produced by FastQC (version 0.11.5)