FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-47_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-47_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7406298
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCAAAAGCTGCATATGATGGAAGGGAACTAAACTCTGAGGCTATAGC599250.8091086802070346No Hit
CCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGT232980.3145701131658489No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT213100.28772809303649405No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT212570.28701248586000727No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG196070.2647341492335307No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA188950.2551207094286511No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC178400.240876076009904No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC177720.2399579385004492No Hit
ATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTT168820.22794113874434974No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT163500.22075806293508576No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA162610.21955638295947585No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC156630.21148217368515285No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC155970.21059104022009378No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC153130.20675646591590022No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA149280.20155818736972236No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT145310.19619788455717013No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG138540.1870570155292158No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA133830.18069756307402157No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG129630.1750267137509185No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG127080.17158369809046303No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT126990.17146217989068224No Hit
GAACAATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAA123300.1664799336996702No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC121850.16452214048097985No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC121080.16348248477174426No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG118920.16056604797700552No Hit
GTACTTTTCTGTGTCTTTGATTTCGAGCAACATAAGCCCGTTAATACAAA118720.1602960075330482No Hit
GGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTA118280.1597019185563422No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG115270.15563780987478495No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG109880.14836021991013595No Hit
CTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATA109740.14817119159936584No Hit
CATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGTTA109730.148157689577168No Hit
CTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCC109500.1478471430666171No Hit
ATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAAGTAGT108520.14652394489122636No Hit
GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT106870.1442961112285787No Hit
GTATAGGCAGAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAG106840.14425560516198513No Hit
CTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACT106280.14349949191890468No Hit
GTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTT105350.14224380385450328No Hit
CAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGA104330.1408665975903211No Hit
GTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGA102820.13882779223844355No Hit
GAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCAT102620.13855775179448626No Hit
CTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTTTG102590.13851724572789265No Hit
CATGAAGACAGTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCA102310.13813918910635248No Hit
GACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTG102030.13776113248481225No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT101940.13763961428503146No Hit
TCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCTATTGAAACAC100610.13584384533271548No Hit
CTTTCTGTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCA100210.13530376444480088No Hit
CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTT99870.13484469569007348No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT98190.13257635596083225No Hit
GTTGTAAGAGGTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTT96480.1302675101649974No Hit
GTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTC96140.12980844141027No Hit
CTACAGCACCCTGCATGGAAAGCAAAACAGAAAGTAGTGAAACCATTTTT94930.1281746967243284No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT94780.12797216639136044No Hit
ACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATC91760.12389455568760532No Hit
GTAGATAAAAGTGCATTAACATTGGCCGTGACAGCTTGACAAATGTTAAA91280.12324645862210784No Hit
GTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTAGA90800.12259836155661033No Hit
GTCCAACATTTTGCTTCAGACATAAAAACATTGTTTTGATAATAAAGAAC90210.12180174224693631No Hit
CTCCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAG89420.12073508249330503No Hit
CCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAAC87900.1186827751192296No Hit
GGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCA87600.11827771445329367No Hit
CATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGT86730.11710303852207946No Hit
CTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCC86590.11691401021130936No Hit
GTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTCTCAGT86100.116252411123614No Hit
CATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGA85160.11498322103701472No Hit
GTCTCAAGGGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGT84960.11471318059305742No Hit
CCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCT84220.11371403095041545No Hit
GACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCT83320.11249884895260763No Hit
GAGTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACTCATTCA83210.11235032670843112No Hit
CAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTT82170.11094611639985322No Hit
TTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGAATCACCATTAGC81400.10990646069061764No Hit
ACATAAGCCCGTTAATACAAACTGGTGTACCAACCAATGGAGCTTCAACA80410.10856976049302904No Hit
GTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTA79160.10688200771829598No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT79080.10677399154071306No Hit
GAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGC78940.10658496322994294No Hit
GTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTTGATAATGTTG78520.10601787829763262No Hit
CTATTAGCATAAGCAGTTGTGGCATCTCCTGATGAGGTTCCACCTGGTTT78330.10576133987587322No Hit
ACCTACTAGTGAAGCTGTTGAAGCTCCATTGGTTGGTACACCAGTTTGTA78060.10539678527653087No Hit
GGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAA77750.10497822258839706No Hit
GTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTT77700.10491071247740774No Hit
GCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGT77540.10469468012224191No Hit
GTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAA76260.10296642128091525No Hit
GTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAA75880.10245334443739638No Hit
GTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAAC75620.10210229186025191No Hit
CATAAGCTTCTTGAGCAGTAGCAAAAGCTGCATATGATGGAAGGGAACTA75360.10175123928310742No Hit
CCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCAAGGCTTTAAGTTT74810.10100862806222488No Hit
GCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTAC74630.10076559166266331No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAG19600.0108.096052
GGGTAGT33900.0102.374111
CCCTAGC62050.0100.978981
GCTCAAT22850.0100.65982
AAGGGTA7900.098.274252
GTCTCAA16600.097.534611
CCTAGCA64600.097.379972
AGGGTAG8050.096.388973
TCAATGT24050.094.95884
CCGATTG353.434752E-582.87653
GAAGGGT9950.078.806611
GCCGACC2800.077.67573145
GCTCGGA809.822543E-1172.604241
TACGGCC502.3045013E-672.5169453
GAATATT24450.068.595411
TGCGCAT4450.068.427244
GGTAGTA51150.068.372972
GAGCTGA7200.067.562271
AGTAGAG53550.065.654335
TAGCATT101200.063.187014
Produced by FastQC (version 0.11.5)