FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-47_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-47_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15066138
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA1241130.8237877550305195No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC737450.4894751395480381No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG508280.3373658199599659No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT479190.31805762034039514No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT463680.3077630113304418No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT439350.29161421460496384No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC434820.28860747193474534No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC380270.25240044927240146No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC376900.25016364512259215No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC343360.22790180204110702No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT316910.2103458762955709No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC290880.1930687213936312No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG284540.18886060913553296No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA275130.18261481475876565No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA273240.1813603459625818No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA268320.17809474465188094No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA264470.17553934525224713No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC263550.17492870435675023No Hit
GTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAA262800.1744308992788995No Hit
TTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAG250130.16602131216374097No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG243490.1616140778745024No Hit
GTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAA238840.15852768639182782No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA237230.15745906482470823No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA236480.15696125974685748No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT234510.15565369174236954No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG233570.15502977604479662No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG229310.15220224320260442No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC228690.1517907243382478No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA227310.15087476299500244No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC223110.14808705455903828No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC218080.14474844183691932No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC217950.1446621556234252No Hit
TTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGC216120.14344751123346938No Hit
GTTTTAAGACAGTGGTTGCCTACGGGTACGCTGCTTGTCGATTCAGATCT214680.14249172548399597No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT213390.1416355007500927No Hit
GTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTG212770.1412239818857361No Hit
TCATTATGCCTTTAGGTAATGTTGCACTATCACCATAATTTTGAAGGTCA209540.1390801013504589No Hit
GAATTAGCTATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGC209260.1388942541213946No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG208000.1380579415906054No Hit
GCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTC207580.13777917074700896No Hit
GCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGAC199360.13232322709376484No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG195990.1300864229439555No Hit
GTGCAGGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGG192880.12802219122113445No Hit
GTATAGCATGGAACCAAGTAACATTGGAAAAGAAAGGTAAGAACAAGTCC190500.1264424897740881No Hit
CTTATTATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAA187570.12449773126995119No Hit
GAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTT183480.12178303424540517No Hit
GTAATAAATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCA182590.12119230555302227No Hit
CTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACAC182020.12081397369385573No Hit
ATGTTAGACTTCTCAGTGGAAGCAAAATAAACACCATCATTAAATGGTAG181220.12028298161081492No Hit
AGGTAATGTTGCACTATCACCATAATTTTGAAGGTCACACTTTTCTAATA172770.11467437773369658No Hit
CTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTG172320.11437569468698615No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA171490.11382479040083134No Hit
TTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGT164910.10945738051782083No Hit
CTTTAATAACAACATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTC164000.10885337702336194No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC163400.10845513296108132No Hit
ATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACA163010.10819627432059895No Hit
CATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCT162140.1076188204302921No Hit
GTTTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCA160910.10680242010261687No Hit
CATAACAGCAGTACCCCTTAATTTAAGGGGAAATTTACTCATGTCAAATA158410.1051430698431144No Hit
ATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCA158010.10487757380159402No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC156570.10392178805212059No Hit
GTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATAT155750.10337752116700379No Hit
TCTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTT154140.10230889959988419No Hit
TTATTACCCTGACAAAGTTTTCAGATCCTCAGTTTTACATTCAACTCAGG153310.10175799531372937No Hit
ATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTG152260.10106106820473833No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA151300.10042387770508938No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA128800.095.578121
TCCTAGG136800.089.894053
TTCCTAG139250.088.518312
CCTAGGT142550.086.355684
CTAGGTA146600.084.5620355
TAGGTAG148100.083.8618856
GCCGACC7000.078.70954145
CAGGTAT64000.073.6298454
GATCCGG1050.069.128831
ATCCGGT1050.069.094842
AGGTAGT180550.068.954717
GCAGGTA69350.068.9017263
TATGCGC100550.063.8800055
GCTGCCC9350.062.803707145
GTAGAGC15650.062.5475853
TGCGCTA104450.061.297597
ATGCGCT104800.061.1580056
CGCTAGT107400.059.4844069
ATATGCG108600.059.212624
AAGTCCG3950.058.730637145
Produced by FastQC (version 0.11.5)