FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-46_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-46_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11417284
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGGAATAGGCTCCTGCTGCACCACACTGTCCCTGCTCTCTTCAACC1006130.8812341008597142No Hit
CCCTAGCATTGGACTCAGATCTGACTCCAATCCTAGGTAACCTTAGTCAA867490.7598041705890823No Hit
TTCCCATACTGTGCACTCGCTGAGAGCTTGTTTCCCCTGGGACATGAGAG790230.6921348369717352No Hit
CCCTAGCATTGCACCTGGTATGCTGCAGTGCTGGTCAATGCTGCATATTG632980.5544050581556875No Hit
CTCCCTAGCATTGGACTCAGATCTGACTCCAATCCTAGGTAACCTTAGTC541940.47466630417531874No Hit
GTTCAGCACCTGACAGCTCCCCACACTCCCACAGCTCCTGCCTCCTGCAC401840.3519576109344394No Hit
ATTCCCATACTGTGCACTCGCTGAGAGCTTGTTTCCCCTGGGACATGAGA395300.3462294535197688No Hit
CTCCCTAGCATTGCACCTGGTATGCTGCAGTGCTGGTCAATGCTGCATAT391420.34283109713308346No Hit
CCCTAGCATTGACCAGCACTGCAGCATACCAGGTGCAATGCTAGGGGATA383650.3360256257092317No Hit
GAGTAAGGTAGGGAGTAAGTCTGGGTCACCCAAGTCATCCTCTCCATTGT375060.3285019449459259No Hit
CCCTAGTAGCTGGGACTACAGGCATGTGTCACCACACCTGGCTGATTTTT363700.3185521179993421No Hit
GGTCAATGCTGCATATTGCATTACTGAAAACACTGCTTCCTCCAGGCCTC354240.3102664346441763No Hit
CCTGTAACCCTTGGCTCTCCTGATGTTCTTGCCCAGCTGGGCAGGAAGGT348760.30546669418050737No Hit
ATGCTGCATATTGCATTACTGAAAACACTGCTTCCTCCAGGCCTCCTGTA324330.28406931105506356No Hit
GTCCCTGCTCTCTTCAACCCAGGTGGGATCCCTGGCCCAGACAGGAAAAA313720.2747763828945658No Hit
CCCTAGCATTGGGTTAGCAGGTGTGAGCCACCATGCCCAACTCAATGTAC309290.2708963007314174No Hit
GTCCAGCAACAGAGGCTCTGCAACTGTGAGTCAGAGTAAGGTAGGGAGTA304630.2668147696072026No Hit
GCATTGGACTCAGATCTGACTCCAATCCTAGGTAACCTTAGTCAAGCTGC294500.2579422566697999No Hit
GGGTTAGCAGGTGTGAGCCACCATGCCCAACTCAATGTACCAATTTTTTT294280.2577495663592147No Hit
GTGCTGGTCAATGCTGCATATTGCATTACTGAAAACACTGCTTCCTCCAG291600.25540224803026707No Hit
GGGCAACAGAGTGAGACCCTGTCTCTGCAAAAAATTTAAAAATCAGCCAG287440.25175864943010967No Hit
GCATTGCACCTGGTATGCTGCAGTGCTGGTCAATGCTGCATATTGCATTA286050.25054119701323013No Hit
GTAGTAGAGAGAGGAGGAGAAGTGCTAGCTTCAGTGCTGATGTCTGTAGA271500.2377973605631602No Hit
GTATGCTGCAGTGCTGGTCAATGCTGCATATTGCATTACTGAAAACACTG267840.23459169448706013No Hit
CCCCTAGGCAGAACTCAAAGCCCTGTCTTGCTGCCTTCAAATGTCTTCAC265760.23276989518698143No Hit
ACTGAAAACACTGCTTCCTCCAGGCCTCCTGTAACCCTTGGCTCTCCTGA261270.2288372611209461No Hit
CCCTAGCATTGCTGCTGATTTCCTGCCCTTCCAGCAGTGGGAAGGAAGGA260590.22824167288822808No Hit
GCTCCAAGCAGCCTGGTGACCTTGGATGTCCTTTTTCCTGTCTGGGCCAG251610.2203764047561574No Hit
GCATATTGCATTACTGAAAACACTGCTTCCTCCAGGCCTCCTGTAACCCT249660.2186684679123336No Hit
CCCTAGCATTGAGTTGGGCATGGTGGCTCACACCTGCTAACCCAATGCTA246440.21584818245740403No Hit
GAGTGAGACCCTGTCTCTGCAAAAAATTTAAAAATCAGCCAGGTGTGGTG242860.21271258558515316No Hit
GTGCAGCAGAGTGGCCAGCAGACAAGTGGCTGGAGAATGATGTGGGAAAT242300.21222210115820891No Hit
GAGCAGGGACAGTGTGGTGCAGCAGGAGCCTATTCCCATACTGTGCACTC236530.20716835983058665No Hit
CCTCTAGTCTCCTCAAATTGGTGAACTCCTAGTCATCCTTTAAAGCCCAA219960.19265527598332494No Hit
CTGCAGTGCTGGTCAATGCTGCATATTGCATTACTGAAAACACTGCTTCC216310.189458368557706No Hit
CTCAAGACCAGCCTGGGCAACAGAGTGAGACCCTGTCTCTGCAAAAAATT214790.18812705368457155No Hit
CTCCCACAGCTCCTGCCTCCTGCACTGTTGTGTCAATTTGGACAGCTGTC211710.18542938933637806No Hit
ATTCTGGAGGCCCCACTCTGCTCCAAGCAGCCTGGTGACCTTGGATGTCC207020.18132158226071982No Hit
CTCTAGTCTCCTCAGTGGCTGGCAGGTGGCATTGTTCAGCACCTGACAGC205360.17986764628084928No Hit
CAATGTGTCCAGCAACAGAGGCTCTGCAACTGTGAGTCAGAGTAAGGTAG204830.17940343780534845No Hit
GTTGTGTCAATTTGGACAGCTGTCGTGCCAGCTCTGAAACACACCCAGTG204600.17920198884428207No Hit
CTACTAGGGAGGCTGAGGTAGGAGGGTCCTTTGAACCCAGGAGGCAGAAG201830.17677584266100416No Hit
CTCCTGCACTGTTGTGTCAATTTGGACAGCTGTCGTGCCAGCTCTGAAAC200950.17600508141866314No Hit
GGGTTGAAGAGAGCAGGGACAGTGTGGTGCAGCAGGAGCCTATTCCCATA186960.1637517293955375No Hit
GGCTCAATGTGTCCAGCAACAGAGGCTCTGCAACTGTGAGTCAGAGTAAG185040.16207006850315714No Hit
TGCTATGGGAGGGCAAGGCAGAAGGATCGCTTGAGCCCAGGCACTCAAGA183930.16109785829974974No Hit
GGGTAGTAGAGAGAGGAGGAGAAGTGCTAGCTTCAGTGCTGATGTCTGTA181830.15925854169870873No Hit
CTTCAACCCAGGTGGGATCCCTGGCCCAGACAGGAAAAAGGACATCCAAG181650.15910088599004807No Hit
CATGAAGACAGTGTGGCTCAATAGCTAGCACCGGGGACGGGAACGTGGGT178130.15601784102068408No Hit
ATGCTATGGGAGGGCAAGGCAGAAGGATCGCTTGAGCCCAGGCACTCAAG176430.15452887043888897No Hit
GATCTGACTCCAATCCTAGGTAACCTTAGTCAAGCTGCTTCCCCTCTAGG173720.15215527615849794No Hit
GAGTTGGGCATGGTGGCTCACACCTGCTAACCCAATGCTATGGGAGGGCA170370.14922112824731346No Hit
GTGTGGTGCAGCAGGAGCCTATTCCCATACTGTGCACTCGCTGAGAGCTT167940.14709277618039457No Hit
TTCCTACCCAATTCTGGAGGCCCCACTCTGCTCCAAGCAGCCTGGTGACC165800.14521842497742896No Hit
CTCCTGATGTTCTTGCCCAGCTGGGCAGGAAGGTGGTGCATCTCTCATGT165290.1447717338028904No Hit
CTCATGTCCCAGGGGAAACAAGCTCTCAGCGAGTGCACAGTATGGGAATA164920.14444766373508797No Hit
CTGCAACTTCTGCCTCCTGGGTTCAAAGGACCCTCCTACCTCAGCCTCCC164620.14418490422065353No Hit
CTGTTGCCCAGGCTGGAGCGCAGTGGCGCGATCTCGGCTCACTGCAACTT156440.1370203281270747No Hit
CCCTAGCATTGGCAGCTCTCCCTTAGGCAGCTCTCCCTAGCATTGGTGCC154300.1351459769241091No Hit
CTGCAACTGTGAGTCAGAGTAAGGTAGGGAGTAAGTCTGGGTCACCCAAG152680.13372707554616317No Hit
TTCCTTACCTGAGAAAGTTCCTACCTTCACCAATGTCAATTATCCCTCGA150510.13182644839175411No Hit
GCACCACACTGTCCCTGCTCTCTTCAACCCAGGTGGGATCCCTGGCCCAG149880.13127465341144182No Hit
GCATTGGGTTAGCAGGTGTGAGCCACCATGCCCAACTCAATGTACCAATT147980.12961050982002376No Hit
GCATTGAGTTGGGCATGGTGGCTCACACCTGCTAACCCAATGCTATGGGA146260.12810402193726636No Hit
CTCCTAGTCATCCTTTAAAGCCCAACTCAAATTCCCCCACCTCTGCAAAG145000.12700043197664174No Hit
CTCCCTAGCATTGCTGCTGATTTCCTGCCCTTCCAGCAGTGGGAAGGAAG144400.12647491294777286No Hit
GTCTCAAGGGCTGTTGTTGTGGTTGTGGGTTCCATAGAGGTGGTAGTGGT142750.12502973561838351No Hit
GAGTAAGTCTGGGTCACCCAAGTCATCCTCTCCATTGTGGGGAGGAGGAA142430.12474945880298677No Hit
GTTCAAAGGACCCTCCTACCTCAGCCTCCCTAGTAGCTGGGACTACAGGC141540.12396993891016463No Hit
GACCAGCACTGCAGCATACCAGGTGCAATGCTAGGGGATAACAGATCATG140900.12340938527937116No Hit
CTCCAATCCTAGGTAACCTTAGTCAAGCTGCTTCCCCTCTAGGCCTCATT139140.12186786279468917No Hit
GTGTCCAGCAACAGAGGCTCTGCAACTGTGAGTCAGAGTAAGGTAGGGAG134290.11761991731133253No Hit
CTCCCTAGCATTGGGTTAGCAGGTGTGAGCCACCATGCCCAACTCAATGT133980.11734839914641695No Hit
CACCCAGTGTTTCCTACCCAATTCTGGAGGCCCCACTCTGCTCCAAGCAG132970.11646377544782105No Hit
GCAACAGAGGCTCTGCAACTGTGAGTCAGAGTAAGGTAGGGAGTAAGTCT131710.11536018548719643No Hit
ACTCAGATCTGACTCCAATCCTAGGTAACCTTAGTCAAGCTGCTTCCCCT131200.1149134943126579No Hit
GTCTCAAGGGCTGTTGTTCTGCCCTTTCCTTACCTGAGAAAGTTCCTACC130840.11459818289533658No Hit
GCTATGGGAGGGCAAGGCAGAAGGATCGCTTGAGCCCAGGCACTCAAGAC130370.11418652632272264No Hit
GCACTGCAGCATACCAGGTGCAATGCTAGGGGATAACAGATCATGGAGAA129350.11329314397364558No Hit
ATATTGCATTACTGAAAACACTGCTTCCTCCAGGCCTCCTGTAACCCTTG129170.1131354882649849No Hit
TTGCATTACTGAAAACACTGCTTCCTCCAGGCCTCCTGTAACCCTTGGCT129000.1129865912068054No Hit
AGCATTGGGTTAGCAGGTGTGAGCCACCATGCCCAACTCAATGTACCAAT128400.11246107217793652No Hit
ACCCAATGCTATGGGAGGGCAAGGCAGAAGGATCGCTTGAGCCCAGGCAC126030.11038527201390454No Hit
CCCAATGCTATGGGAGGGCAAGGCAGAAGGATCGCTTGAGCCCAGGCACT124800.10930795800472336No Hit
GGTAGGGAGTAAGTCTGGGTCACCCAAGTCATCCTCTCCATTGTGGGGAG123170.10788029797629628No Hit
CCTGATGTTCTTGCCCAGCTGGGCAGGAAGGTGGTGCATCTCTCATGTCC122680.10745112410272006No Hit
CTCTGCAACTGTGAGTCAGAGTAAGGTAGGGAGTAAGTCTGGGTCACCCA121920.10678546666615284No Hit
GTCCAGGAGAACAGGACTGGCAGCCACAAGGACTAAAGAGCCTCATGGAT120710.10572566995793395No Hit
GCACTGTTGTGTCAATTTGGACAGCTGTCGTGCCAGCTCTGAAACACACC120660.10568187670552821No Hit
TGCTAACCCAATGCTATGGGAGGGCAAGGCAGAAGGATCGCTTGAGCCCA120640.10566435940456591No Hit
GTGTTTCCTACCCAATTCTGGAGGCCCCACTCTGCTCCAAGCAGCCTGGT119680.10482352895837574No Hit
ACTCTGCTCCAAGCAGCCTGGTGACCTTGGATGTCCTTTTTCCTGTCTGG119300.10449070024009213No Hit
CAGCAACAGAGGCTCTGCAACTGTGAGTCAGAGTAAGGTAGGGAGTAAGT118490.10378124955111917No Hit
CTCCTACCTCAGCCTCCCTAGTAGCTGGGACTACAGGCATGTGTCACCAC116780.10228352031884291No Hit
CCTTAGTCAAGCTGCTTCCCCTCTAGGCCTCATTTCCTCCTCCCCACAAT116580.10210834730921994No Hit
AGGTAACCTTAGTCAAGCTGCTTCCCCTCTAGGCCTCATTTCCTCCTCCC116240.10181055319286093No Hit
CCTCTAGTCTCCTCAGTGGCTGGCAGGTGGCATTGTTCAGCACCTGACAG115610.10125875821254862No Hit
GCACCTGACAGCTCCCCACACTCCCACAGCTCCTGCCTCCTGCACTGTTG115530.10118868900869944No Hit
TCCTACCTCAGCCTCCCTAGTAGCTGGGACTACAGGCATGTGTCACCACA115200.10089965354282157No Hit
CTCAAGGGCTGTTGTTGTGGTTGTGGGTTCCATAGAGGTGGTAGTGGTTG114980.10070696323223632No Hit
CTCCCTCTGTTGCCCAGGCTGGAGCGCAGTGGCGCGATCTCGGCTCACTG114880.10061937672742483No Hit
TCCCCATCCCCTCTGCTCCTCAGTGGCTGGCAGGTGGCATTGTTCAGCAC114780.10053179022261335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGAAG92050.095.253471
AGACAGT116100.080.617486
TCTCAAG109750.078.9274442
GTCTCAA116500.075.200211
GGGTAGT76250.074.7252661
AGGGTAG35300.074.562163
ACGAGGT17200.072.126672
AAGGGTA36700.071.7577442
GGTAGTA79050.071.584822
ATGAAGA122600.071.187442
CGAGGTC18500.067.0213
CCCTAGC1007750.063.9440351
CCTAGCA1051350.061.6146162
CTAGCAT1046300.061.5518463
GACGAGG20550.061.1043321
CTGCGCG6700.058.430664145
TTGGACT253650.058.0362139
GGTCTGC61500.055.8243561
TCAAGGG156100.055.356254
TAGCATT1167300.055.2962884
Produced by FastQC (version 0.11.5)