FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-46_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-46_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11985048
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC1968121.6421461140581164No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC1820231.5187506967014233No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG1454431.2135370671857133No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC1352791.1287313993235573No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT685930.5723214458548685No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT664640.554557645492951No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC657280.5484166604923068No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG617390.51513352303637No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG570670.4761516182496724No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC528510.44097445416989567No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC467900.3904031089404064No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA455700.38022375880346915No Hit
ACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACG450070.37552623902716115No Hit
CTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGG401600.3350841815568865No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT397720.33184681446415565No Hit
ATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCC349740.2918135997452826No Hit
AACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCA341820.2852053658858938No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC336980.28116700074960066No Hit
GTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCC313610.26166770462663147No Hit
GTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGGAACGC309180.2579714324047763No Hit
CCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGA292620.24415421615332702No Hit
GGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACC287010.23947338383626No Hit
AACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCA286340.2389143539516905No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG282760.23592729874757282No Hit
GTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAG275750.2300783442836441No Hit
CTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTC267550.22323648599488297No Hit
TTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCG266870.22266911238069304No Hit
CCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGA264760.22090858543078007No Hit
CTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATT261690.2183470604373049No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA258300.2155185360959756No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC256060.21364954066099692No Hit
GGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGG253880.21183060760374095No Hit
ATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGA241920.20185150697769422No Hit
TTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACC237600.19824701578166395No Hit
GATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAA232290.1938164953532101No Hit
CTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCGA229480.19147190732986635No Hit
GCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTC229180.19122159544125314No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA228850.1909462523637786No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT227880.1901369105905959No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG222940.18601510815809832No Hit
TCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGG220730.184171143911981No Hit
CTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATT219270.1829529593873967No Hit
GGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGC215990.18021621607189225No Hit
CTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCT214100.178639251173629No Hit
GTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTG207700.17329926421654715No Hit
AAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAAT204680.17077945787117413No Hit
CCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCG203940.17016202187926155No Hit
CCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAG203810.17005355339419584No Hit
ACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCAT200550.16733349753793228No Hit
CTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAA200430.16723337278248698No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT198350.16549787702143537No Hit
TGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTG196400.1638708497454495No Hit
GGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC196390.16386250601582905No Hit
CATCTGGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAAT195620.16322003883505515No Hit
AAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCAC193980.1618516671773029No Hit
CCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTA192560.1606668575712004No Hit
CCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGG191350.1596572662871271No Hit
GTTCCTAGGTAGTAGAATGGAATGGAACGAAATGGAATGGAATGGAATGG188210.1570373351863088No Hit
GAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTG184880.15425887322270215No Hit
CACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTC183930.15346621890876033No Hit
ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTC179590.1498450402534892No Hit
GGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAG173290.14458849059261172No Hit
GATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAA170740.14246083953939942No Hit
CTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGA170610.14235237105433368No Hit
GTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCA169540.14145959198494656No Hit
CAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAA169140.14112584280012896No Hit
ATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTC169080.14107578042240632No Hit
CAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCA169000.14100903058544279No Hit
ACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATA168690.14075037496720913No Hit
GAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTT163080.1360695426501421No Hit
ATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCA161750.1349598266106235No Hit
GCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAG160060.13354973630476907No Hit
GATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGG157290.1312385231999071No Hit
CTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGT157090.1310716486074983No Hit
CCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTG154910.12925271555024226No Hit
ATTATATTCCAATCCATTCTGGTTGTTTCCATTCCATTCCATTCCATTTC154380.12881049788035895No Hit
GAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGAC154250.12870202939529318No Hit
GTAATGGAGTGTAAGGGAATTGAATAGAATCAATCCGAATGTAATGGAAT154100.1285768734509866No Hit
CCATTATATTCCAATCCATTCTGGTTGTTTCCATTCCATTCCATTCCATT153980.12847674869554132No Hit
GAATAGAATGGAATGGAATGCAATGGAATGGATTCAACTTGAATGGAATG152900.12757562589653376No Hit
AATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGC151570.1264659098570152No Hit
ATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGA150550.12561484943573026No Hit
GAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTGGGGC148360.1237875726488538No Hit
GTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCC148360.1237875726488538No Hit
TTATCAGACATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAACACGA147220.12283638747212361No Hit
CCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATG144830.12084223609283834No Hit
GCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAAC140350.11710424522288103No Hit
CTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGAT140060.11686227706388828No Hit
ATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTT134550.11226488204302561No Hit
ATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTCTTCATAGAACG132890.11087982292603249No Hit
GAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACG132530.11057944865969666No Hit
GAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGT132080.11020398082677682No Hit
GAATAGCAGAAAGGCTAAAAAGCACAAATAGAAGTCAATTAATGAAAGTT131860.11002041877512714No Hit
AGGTAGTAGAATGGAATGGAACGAAATGGAATGGAATGGAATGGAATGGA130820.10915267089460134No Hit
TAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAG130580.10895242138371077No Hit
GAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTC129430.10799289247736012No Hit
GTTGTGATGATTCCTAAGAAAACAAGAAATTTCATGTTCGTTTAGGCGTG127270.10619064687934501No Hit
GAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAA125350.10458865079222045No Hit
CACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATG123870.10335377880839526No Hit
TCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACT123750.10325365405294998No Hit
CACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCA122970.10260284314255563No Hit
AAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAA121740.10157656439924145No Hit
GTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTGG121070.10101753451467195No Hit
ATTCTATTCCGTACCATTCCATTCCATTCCATACCATTCCATTCCATTCC120860.1008423161926427No Hit
GTAATGGAGAGTAAGGGAGTTGAATAGAATCAATCCGAATGTAATGGAAT120190.10028328630807318No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA277850.0138.149321
TCCTAGG311050.0123.456373
TTCCTAG316000.0121.5782852
CCTAGGT321050.0119.496684
CTAGGTA330800.0116.608735
TAGGTAG349150.0110.92786
AGGTAGT433850.089.397817
TATGCGC3100.072.49425
ATATGCG3600.066.453854
TGCGCTA3350.064.933727
TTGTAGA495450.063.1062772
AGATCTG536600.058.8418166
ATCTGTT543650.057.7945338
GATCTGT562950.055.835857
CTTGTAG569250.055.101991
GTAGAGC25700.054.452763
TTCCGAG1550.051.4820862
TTTCGCG4300.048.89205145
ATGCGCT4450.048.8775946
GCAGGTA11900.048.1366463
Produced by FastQC (version 0.11.5)