FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-45_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-45_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14112103
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT824090.5839597400897655No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG735380.5210988043383754No Hit
ATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTT713580.5056510712825721No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC600770.4257125957768307No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC553920.392514141939015No Hit
GTAGCAAAAGCTGCATATGATGGAAGGGAACTAAACTCTGAGGCTATAGC546460.38722789934285484No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG523820.37118493253627755No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC469800.33290573346864033No Hit
GTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTT459950.3259259091292063No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC447220.3169052833585469No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG437150.3097695644653387No Hit
GTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTAGA415670.2945485871241161No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG412080.292004671451165No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT402560.2852586889423922No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA401380.28442252724487627No Hit
CCCTAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAG396590.2810282776422479No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA392770.27832138129944206No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA387060.2742752090173945No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT380710.26977552530618576No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC362760.25705594694143036No Hit
GTCTCAAGGGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGT354690.25133745126435086No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG321130.22755644569771066No Hit
AATTAAATGTTGGTGATTATTTTGTGCTGACATCACATACAGTAATGCCA317950.22530306078406598No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG316980.22461570752424354No Hit
CCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGT314890.22313470926338905No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC312090.22115059676080878No Hit
CAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTT307330.2177776055064224No Hit
TTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGAATCACCATTAGC299160.21198824866853652No Hit
CCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCT294910.2089766493342629No Hit
CTCAAGGGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTG292390.20719094808194072No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG292270.20710591468897302No Hit
GGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTA289810.20536273013313466No Hit
GTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACATCA286780.20321563696069964No Hit
CTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCC283630.20098351039529688No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA281870.19973635396510359No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT281550.19950959825052297No Hit
GTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTA272600.1931675243583469No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT270470.19165818163316978No Hit
CTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACT270440.19163692328492782No Hit
GTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGA269490.19096374225726667No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT267950.18987248038084756No Hit
CAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGA265600.1882072431018963No Hit
GTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTT262020.18567041354502584No Hit
AGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACAT260940.18490511300831633No Hit
GCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTA260780.18479173515102604No Hit
GCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTAC259710.18403352073039717No Hit
GTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAAC254340.1802282763950915No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA250360.1774080021949953No Hit
TCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAA242480.1718241427234481No Hit
ACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACT230030.1630019282030467No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT224300.15894158368883787No Hit
TCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCTATTGAAACAC223780.1585731056526444No Hit
GAAGGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACC221580.1570141601149028No Hit
GTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCA218560.1548741530585484No Hit
CATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGTTA218170.1545977945314033No Hit
CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTT215900.1529892461810972No Hit
ACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGC214870.15225937622479088No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG214010.1516499702418555No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG213880.15155785073280714No Hit
ATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTG213560.15133109501822656No Hit
GTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTTGATAATGTTG213090.1509980475624363No Hit
GACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTG212150.1503319526508558No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC210130.14890055720256576No Hit
CACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATC207770.14722823380753386No Hit
CACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATAC205260.1454496186712923No Hit
CATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGTTGCATGG202040.14316788929332502No Hit
CTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTATACATTTG201240.14260100000687354No Hit
GCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGT199170.1411341739781803No Hit
CACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCAC196590.13930595602937423No Hit
GGCTTATACCCAACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGC192210.1362022371860523No Hit
AGTAAATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTA191040.135373161604617No Hit
CATCACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAG188630.13366540762918183No Hit
GACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCT188480.1335591158879722No Hit
GTTGTAAGAGGTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTT186000.13180175909997255No Hit
GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG184550.13077427226827923No Hit
GAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTCCCTGG183280.12987433552603747No Hit
GAGTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACTCATTCA182890.12959797699889236No Hit
GTCCATACTAATTTCACTAAGTTGAACAATTTTACTATCTGCATCTACAA182500.12932161847174728No Hit
GTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCT182190.1291019488732473No Hit
GTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGGTA178800.1266997555219091No Hit
CCATTAGCAACAGCCTGCTCATAAGCTTCTTGAGCAGTAGCAAAAGCTGC176580.12512663775200622No Hit
TGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGA175340.1242479593580064No Hit
GCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGT171960.12185285212274882No Hit
GACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTC171130.1212647044880554No Hit
ACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGC170870.12108046546995867No Hit
ATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGAAAAGCAT170760.12100251819307158No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT169590.12017344261163627No Hit
GCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAA169360.12001046194178146No Hit
TAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCT168880.11967032836991055No Hit
CTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAG167390.11861449707389465No Hit
GTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACTCATTCACA164770.116757934660766No Hit
GTAGATAAAAGTGCATTAACATTGGCCGTGACAGCTTGACAAATGTTAAA164030.11623356207079838No Hit
ATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTAT160130.11346997679934734No Hit
GTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAA159340.11291017362897648No Hit
GTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTC156530.1109189750103156No Hit
GGGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGAT155360.11008989942888031No Hit
CATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGT153740.10894194862381604No Hit
GGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGT151380.10726962522878411No Hit
GTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTT151250.10717750571973574No Hit
GCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAAC151200.10714207513933253No Hit
ATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTT151180.10712790290717124No Hit
GCTCATAAGCTTCTTGAGCAGTAGCAAAAGCTGCATATGATGGAAGGGAA150430.10659644420112296No Hit
GCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGA150430.10659644420112296No Hit
CACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAAT149550.10597286598602633No Hit
ATTTTGTGCTGACATCACATACAGTAATGCCATTAAGTGCACCTACACTA149480.10592326317346182No Hit
ACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGTAGACATAAACAAGCTTT146790.10401709794776867No Hit
CCTTTTGATAATTTGCAACATTGCTAGAAAACTCATCTGAGATATTGAGT146560.10385411727791387No Hit
TTGCATGGCTGCATCACGGTCAAATTCAGATTTAGCCACATTCAAAGACT145660.10321636683065592No Hit
GTATAGATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAA144750.10257153026731736No Hit
GTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTG144420.10233768843665612No Hit
CCCTAGCATTGTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTA144320.10226682727584968No Hit
ACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCGTCAGAGA143240.10150152673914015No Hit
GCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTATACAT142420.10092046522052739No Hit
GTATTATCTTTCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGA142170.10074331231851127No Hit
CAACAGCATCGTCAGAGAGTATCATCATTGAGAAATGTTTACGCAAATAT141220.10007013129085013No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGTA30500.0117.262582
GCTCAAT44450.0114.5717852
GTCTCAA49850.0112.5495761
GAAGGGT33800.0105.652061
CCCTAGC151450.0104.859451
TCCCTAG41700.0104.382462
AGGGTAG34400.0103.9100343
TCAATGT49050.0103.897934
CCTAGCA156300.0102.0654142
GGGTAGT121300.0101.602981
CTCCCTA44950.096.883791
GCTCGGA1200.096.776141
GGTAGTA147500.082.579262
GAATATT12150.077.659861
GCCGACC5050.074.652985145
TAGCATT239900.067.8994
AGTAGAG191750.063.8148045
ACGAGGT2300.063.083312
GAAGACA23000.063.0365944
GCTGCCC7750.061.741535145
Produced by FastQC (version 0.11.5)