FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-44_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-44_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7732983
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT792691.0250766101516064No Hit
CCCTAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAG574120.7424301850915747No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC505760.6540296286698161No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC413650.5349164740178531No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA379120.4902635890962129No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA356170.46058552049060497No Hit
CCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGT335350.4336618870104848No Hit
CCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCT283640.3667924784006379No Hit
GCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTA271110.3505891581553975No Hit
CTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCC266410.34451129661089386No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT265900.3438517839752137No Hit
GGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTA263740.3410585539888036No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT256410.33157967630343943No Hit
CCCTAGCATTGTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTA253660.3280234807188895No Hit
CTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACT252080.32598028471031165No Hit
GCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTAC251380.3250750712887899No Hit
CAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGA249930.323199986344209No Hit
GTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTT238320.30818637516725433No Hit
TTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGAATCACCATTAGC237400.30699666609896853No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA231320.2991342409520362No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC228000.29484094300996133No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG224910.29084507233495793No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT221660.2866422957350352No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG213110.2755857603721617No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC210910.2727408039045217No Hit
CTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTATACATTTG207350.2681371470750679No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT207290.26805955735322323No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG199450.25792116703217893No Hit
ATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTG196790.254481356030396No Hit
GTAGCAAAAGCTGCATATGATGGAAGGGAACTAAACTCTGAGGCTATAGC195750.2531364675184207No Hit
GTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGA195630.2529812880747313No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT194910.2520502114125946No Hit
CATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGTTGCATGG194890.2520243481719797No Hit
GCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGT184610.23873064249591652No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA184570.2386789160146867No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA180700.23367437895570184No Hit
CCCTAGCATTGTCATCAGTGCAAGCAGTTTGTGTAGTACCGGCAGCACAA177370.22936814939331948No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG176720.22852759407333495No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG175340.22674303047090627No Hit
AGTAAATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTA175070.22639387672260497No Hit
GTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAAC173620.22451879177802408No Hit
GTCCATACTAATTTCACTAAGTTGAACAATTTTACTATCTGCATCTACAA171750.22210057878053008No Hit
ACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGC170100.2199668614298001No Hit
GACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTC165700.21427694849452017No Hit
TAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCT165390.21387606826498906No Hit
ACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGC150470.19458209076626706No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC148730.19233198883276997No Hit
ACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGTAGACATAAACAAGCTTT148650.19222853587031033No Hit
GCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTATACAT146660.1896551434291269No Hit
GCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAAC146090.18891804107160198No Hit
TCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCTATTGAAACAC145180.18774126362362364No Hit
GTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAA144950.18744383635655218No Hit
GTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTT144710.18713347746917328No Hit
ATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGAAAAGCAT141690.18322812813632203No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG141460.1829307008692506No Hit
CTCCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAG139010.17976245389392426No Hit
CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTT138280.17881844561148005No Hit
TGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGA137570.1779003005696508No Hit
ATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTT137250.17748648871981226No Hit
GCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGA136350.17632264289214136No Hit
CACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAAT136240.17618039506875938No Hit
CCATTAGCAACAGCCTGCTCATAAGCTTCTTGAGCAGTAGCAAAAGCTGC135070.1746673954927872No Hit
GCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAA134480.17390442989464738No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG133870.17311560105589266No Hit
CTCCCTAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTC132200.17095602046454775No Hit
GTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGGTA130370.1685895339482836No Hit
CTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAG130250.16843435450459415No Hit
GTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTCTCAGT130090.1682274485796749No Hit
GTCCAACATTTTGCTTCAGACATAAAAACATTGTTTTGATAATAAAGAAC129590.16758086756430216No Hit
GACTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGC129510.1674774146018425No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG126900.16410226170159692No Hit
GACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTG126560.16366258661114347No Hit
ATTTTACTATCTGCATCTACAACCTGTTGGATTTCCCACAATGCTGATGC125090.16176163842594765No Hit
TTGCATGGCTGCATCACGGTCAAATTCAGATTTAGCCACATTCAAAGACT124080.16045554477489476No Hit
GTGTAGTACCGGCAGCACAAGACATCTGTCGTAGTGCAACAGGACTAAGC122850.15886495547707788No Hit
GTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCC122340.15820544284139768No Hit
TGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCT121880.15761058830725477No Hit
CTTGTCCTCAGATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAG120090.15529582827222044No Hit
GCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGT119730.15483028994115208No Hit
CATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGTTA119220.1541707773054719No Hit
GTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCT118780.1536017860119439No Hit
GTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTTGATAATGTTG118580.1533431536057948No Hit
GGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGTTGCATGGCTGCAT118140.15277416231226681No Hit
GACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCT118110.15273536745134447No Hit
GGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGT118100.152722435831037No Hit
GAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGC118080.1526965725904221No Hit
CCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAAC117920.15248966666550282No Hit
GTGAAAAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTC117670.15216637615781647No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC117630.15211464967658664No Hit
GACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACA113920.14731701854252102No Hit
CATAAAAACATTGTTTTGATAATAAAGAACTGACTTAAAGTTCTTTATGC111830.14461430989826307No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT111660.14439447235303635No Hit
ATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTATGACA109510.14161417398693363No Hit
GTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTT109000.14095466135125345No Hit
GTTGTAAGAGGTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTT108060.13973908904235274No Hit
GTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGAT106590.13783814085715693No Hit
GCTCATTATTCTGTAATTTGACAGCAGAATTGGCCCTTAAAGCTGTTACA106340.13751485034947056No Hit
ATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTT106070.1371656966011693No Hit
GCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGT105920.1369717222965575No Hit
CACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATC104930.13569149188611948No Hit
CAATAAGAGGCCATGCTAAATTAGGTGAATTGTCCATACTAATTTCACTA104540.13518715869412878No Hit
ATAAGAGGCCATGCTAAATTAGGTGAATTGTCCATACTAATTTCACTAAG104470.1350966373519766No Hit
GTGCAAGCAGTTTGTGTAGTACCGGCAGCACAAGACATCTGTCGTAGTGC103780.13420435555076224No Hit
CCTCAGATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATC103620.13399744962584295No Hit
GCTCATAAGCTTCTTGAGCAGTAGCAAAAGCTGCATATGATGGAAGGGAA103390.13370002235877151No Hit
GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG102360.1323680654671037No Hit
CAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGG102330.13232927060618133No Hit
GTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGAAAAGCATTG100970.13057057024436755No Hit
GTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTC100830.13038952756006317No Hit
GAGTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACTCATTCA100150.12951017737915627No Hit
ATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTAT99820.12908343390901028No Hit
GTCCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAA99480.12864375881855683No Hit
TTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAAT99180.1282558102093332No Hit
ATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTC99150.12821701534841082No Hit
CTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTATACATTTGGGTCATA98730.12767388729549772No Hit
GTCATAGCTTGATCAGCCATCTTTTCCAACTTACGTTGCATGGCTGCATC98320.12714369086289212No Hit
CATTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGAATCACCATTA96980.12541085374169322No Hit
GGACTAAGCTCATTATTCTGTAATTTGACAGCAGAATTGGCCCTTAAAGC96450.12472547786539813No Hit
GTTACAATAAGAGGCCATGCTAAATTAGGTGAATTGTCCATACTAATTTC96040.12419528143279249No Hit
GCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTA95920.12404010198910304No Hit
ACATAATACCTCTTACAACAGCAGCCAAACTAATGGTTGTCATACCAGAC95900.12401423874848813No Hit
CATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGT94950.12278573481927996No Hit
TCATTATCCAACTTTCTAAGCATAGTGAAAAGCATTGTCTGCATAGCACT94720.1224883075522085No Hit
AGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTA93230.1205614961263978No Hit
GCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTC92870.12009595779532943No Hit
GCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCA92360.11943644515964927No Hit
ATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCT92260.11930712895657472No Hit
GTCTCAAGGGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGT92210.11924247085503745No Hit
GTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTA91320.11809155664767398No Hit
CAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAA90660.11723806970738201No Hit
GTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACTCATTCACA90220.116669078413854No Hit
CTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGC89520.11576386499233218No Hit
GTATAGATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAA89230.11538884800341602No Hit
GCAACAGCCTGCTCATAAGCTTCTTGAGCAGTAGCAAAAGCTGCATATGA89230.11538884800341602No Hit
ATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTT89020.11511728397695947No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC88180.1140310278711333No Hit
GAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGC85960.11116020816287842No Hit
ATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTA85490.11055242200842805No Hit
TTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACAC85210.11019033663981932No Hit
GCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAA85200.11017740501951187No Hit
GCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTA85000.10991877261336278No Hit
ACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCGTCAGAGA83860.10844456789831297No Hit
AGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAACATTGT83530.10801782442816697No Hit
AATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAA82340.10647896161157991No Hit
CTCTTAGGGAATCTAGCCCATTTCAAATCCTGTAAATCGGATAACAGTGC82130.10620739758512336No Hit
GTCTCAGTCCAACATTTTGCTTCAGACATAAAAACATTGTTTTGATAATA82080.10614273948358609No Hit
GTTCAAGGGAACACAACCATCTCTTGCATTGTTGATAATGTTGTTGAGTG81580.10549615846821338No Hit
TCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTATCAAC81330.10517286796052701No Hit
GCATAAGTGCTATTGAAACACACAACAGCATCGTCAGAGAGTATCATCAT81150.10494009879499283No Hit
CAACAGCATCGTCAGAGAGTATCATCATTGAGAAATGTTTACGCAAATAT80910.10462973990761391No Hit
CTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTT80870.10457801342638409No Hit
AGCTTGATCAGCCATCTTTTCCAACTTACGTTGCATGGCTGCATCACGGT80700.10435817588115737No Hit
TACTAGTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATA80000.10345296245963558No Hit
CTGTTTTGCTTTCCATGCAGGGTGCTGTAGACATAAACAAGCTTTGTGAA79860.1032719197753312No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG79410.10268999686149576No Hit
CTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAACGT78900.10203048422581557No Hit
GTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTG78470.10147442455259505No Hit
ATGATGCACTCAACAACATTATCAACAATGCAAGAGATGGTTGTGTTCCC77920.10076318543568503No Hit
ATGCAGGGTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAA77920.10076318543568503No Hit
AACTAATGGTTGTCATACCAGACTATAACACATATAAAAATACGTGTGAT77330.10000021983754522No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCCTA55800.0115.911261
GCTCAAT41100.0115.802852
TCCCTAG56050.0114.946182
TCAATGT43100.0111.01634
CCCTAGC212700.0106.923961
GTCTCAA15350.0105.9306951
CCTAGCA217600.0104.662152
AGGGTAG8250.0104.5937653
AAGGGTA8400.0101.920132
GGGTAGT27300.094.926551
GGCTCAA51300.093.5332951
CCCGAGC406.0758975E-790.738621
GGGCTCA1900.087.873191
TAGCATT260000.087.721384
GACGAGG1100.085.789231
ACGAGGT1100.085.744812
CTCCTAG2100.079.504311
GGTAGTA34500.075.7077942
CGAGGTC1250.075.412983
GAAGGGT11600.073.2166751
Produced by FastQC (version 0.11.5)