FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-44_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-44_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7135057
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC531930.7455161185117372No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT420610.5894977433256665No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC410700.5756085760772479No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG378520.530507324608619No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG351670.49287623070145054No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA314120.4402487604513881No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT292850.41043820673051384No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC285330.39989869737550804No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT272070.3813144029543142No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA271320.3802632550798123No Hit
GAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGAC227170.318385683534133No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC225370.31586292863532833No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT202650.28402015569041705No Hit
GAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTG201960.2830530996458753No Hit
GTGTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGT196880.275933324709249No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC194510.2726116974258229No Hit
ATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTCTTCATAGAACG191910.26896771812754966No Hit
TTATCAGACATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAACACGA191060.2677764172031141No Hit
GAATAGCAGAAAGGCTAAAAAGCACAAATAGAAGTCAATTAATGAAAGTT190940.26760823354319385No Hit
CTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCT187240.26242257069565106No Hit
ATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGA186500.2613854381261425No Hit
CACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCA179820.25202321439057884No Hit
TCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACT178940.2507898675511632No Hit
ATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATT176250.2470197505079497No Hit
GTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGTTC169950.2381901083621336No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC160750.22529602776824348No Hit
ATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTG159060.22292744122436584No Hit
CTGCTATAGTAACCTGAAAGTCAACGAGATGAAACATCTGTTGTCACTTA157970.22139977298008973No Hit
ATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAA151550.21240194717435334No Hit
CTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGA149030.2088700903160269No Hit
GTATAATTACCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCCTC143210.20071318280989206No Hit
ACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGT141090.19774193815129998No Hit
CTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTT139100.19495289245762157No Hit
ATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTAC137960.19335514768837866No Hit
ATTTAGAACCAGCCTCATCCACGCACAATTCAATTAAAGGTGCTGATTTT134820.18895434192046398No Hit
GTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAA134010.18781910221600193No Hit
CACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATG133170.18664181659655976No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT131090.18372663315794113No Hit
CTCTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACT130960.1835444341930275No Hit
GATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAG130920.18348837297305404No Hit
GTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTC129200.1810777405141963No Hit
GTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCC129180.1810497099042096No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG126300.17701330206612223No Hit
ACGTAATGCGGGGTGCATTTCGCTGATTTTGGGGTCCATTATCAGACATT125290.175597756261793No Hit
GTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACA124410.17436440942237744No Hit
ACTACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTG122800.17210794531844664No Hit
ACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGATGATTCCT122500.1716874861686459No Hit
GTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCA120600.16902457821990768No Hit
GGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAA120340.16866018029008037No Hit
GTTGTGATGATTCCTAAGAAAACAAGAAATTTCATGTTCGTTTAGGCGTG114680.16072751766383928No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC114260.1601388748541182No Hit
CCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCA111750.15662103330078514No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC110990.15555587012128985No Hit
ATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGAT110290.15457479877175473No Hit
GCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTT109200.15304713052747862No Hit
GTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTA108430.15196795204299No Hit
CTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACTACCCAATTTA107670.1509027888634947No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA107460.15060846745863418No Hit
CTGTATAATTACCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCC106200.14884253902947095No Hit
GAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTAC106180.14881450841948424No Hit
TAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAG105100.1473008554802015No Hit
CTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAA103040.14441370265156958No Hit
GTTTAGGTGAAACTGATCTGGCACGTAACTGATAGACGTGTTTTACGCCG102230.1432784629471075No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG99400.13931213163398695No Hit
GACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAAC99060.13883561126421276No Hit
ATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGTCACGC98700.13833106028445183No Hit
GTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTA97820.13709771344503624No Hit
ATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAA97550.13671930021021556No Hit
GTACTGAATGGGTGATTTAGAACCAGCCTCATCCACGCACAATTCAATTA95770.13422457592139767No Hit
GTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCT95450.13377608616161019No Hit
ATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCT95200.1334257035367762No Hit
AAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCT93700.1313234077877724No Hit
CTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAAC93460.1309870404679318No Hit
GACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCG93460.1309870404679318No Hit
CGTCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGA92360.12944535691866232No Hit
GTTTTAGATTTCATCTAAACGAACAAACTAAAATGTCTGATAATGGACCC91610.1283942090441604No Hit
GTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCC89750.12578736231539567No Hit
ACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACG88120.12350286760147818No Hit
GGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAAACTACAT88090.12346082168649809No Hit
CTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTC87590.12276005643683016No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA87380.12246573503196961No Hit
ATCATAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTC86680.12148466368243449No Hit
GTTCAAGTGAGAACCAAAAGATAATAAGCATAATTAAAACAAGGAATAGC86330.12099412800766693No Hit
GGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAATGCAGC86210.12082594434774663No Hit
GTCTTTCTTTTGAGTGTGAAGCAAAGTGTTATAAACACTATTGCCGCAAC86090.12065776068782633No Hit
GATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAAATTATTCT86030.12057366885786618No Hit
GAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAA85150.11934032201845059No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC84010.11774257724920768No Hit
CTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTA83390.11687362833961944No Hit
TTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCG83010.11634104674987181No Hit
ATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCC82620.11579444985513081No Hit
GAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAG82330.1153880060103234No Hit
TAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTAC81790.11463117954068201No Hit
GTAATATCTCTGCTATAGTAACCTGAAAGTCAACGAGATGAAACATCTGT81290.11393041429101408No Hit
GTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGGAACGC79960.11206637872689734No Hit
ATTCAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAATATACCATTTA79920.11201031750692392No Hit
GTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAG79480.11139364408721614No Hit
CCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTG78110.10947354730312597No Hit
TAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAAT77650.10882884327343145No Hit
CATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTACAGTCATGTA77350.1084083841236307No Hit
AACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCA77270.10829626168368381No Hit
TCCACGCACAATTCAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAAT77130.10810004741377678No Hit
GATTTTGGGGTCCATTATCAGACATTTTAGTTTGTTCGTTTAGATGAAAT76620.10738526685911551No Hit
GTTTAGATGAAATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTC75680.10606782818973975No Hit
GGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC75040.1051708486701648No Hit
GTCCATTATCAGACATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAA74710.10470834360538396No Hit
CTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTG74560.10449811403048356No Hit
GTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGT74130.10389545591576914No Hit
AGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGG73770.10339090493600822No Hit
ATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAACAGGAAACTGTATAA73040.10236778767149302No Hit
TAATTAAAACAAGGAATAGCAGAAAGGCTAAAAAGCACAAATAGAAGTCA72900.10217157340158599No Hit
TTTATATACTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATTC72490.10159694589685828No Hit
GTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACATACGAGGGC72170.1011484561370708No Hit
AACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCA72120.101078379612104No Hit
ATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGC71940.10082610412222356No Hit
GCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGTTTCACCTAAACTG71770.10058784393733645No Hit
ACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAACAGGAAACTG71710.1005037521073763No Hit
GTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTT71550.10027950722748255No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA65200.0138.719571
TCCTAGG74400.0120.749233
CCTAGGT75900.0118.053114
TTCCTAG78100.0115.9284742
CTAGGTA79750.0112.7168055
TAGGTAG82550.0109.43666
AGGTAGT99050.091.357267
TCGGACT608.3127816E-1084.576074
CAGGTAT3750.083.12624
TATGCGC7700.067.785925
TGCGCTA7800.066.937
ATGCGCT8300.062.894996
CGCTAGT8950.058.33669
CGAGACT502.0119369E-457.9950264
TTGTAGA106750.055.252162
AGGAGTA4750.050.373637
GCCGACC1600.049.84017145
GCAGGTA6450.049.462833
GACTCGC604.9174944E-448.392271
ACCTCCG450.009644275548.329865145
Produced by FastQC (version 0.11.5)