FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-43_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-43_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21657182
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC4205361.941785408646425No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT3945391.8217467073971119No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT2651751.2244206102160475No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA1953740.9021210608102199No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG1705190.7873554371016507No Hit
ATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGA1456510.6725297871163478No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA1393800.6435740347012829No Hit
ATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTCTTCATAGAACG1252930.5785286377516705No Hit
GAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTG1232030.5688782594152831No Hit
CTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGA1184270.5468255288245719No Hit
GAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGAC1160820.5359977119830271No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT1142370.5274785980927713No Hit
TCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACT1138590.525733218661597No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT1116880.5157088304471006No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC1113240.5140280946985624No Hit
CACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCA1074740.4962510819736381No Hit
CTCTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACT1024410.4730116780659644No Hit
ATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATT983270.4540156701827597No Hit
TTATCAGACATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAACACGA966660.4463461589785781No Hit
GTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAA936950.4326278460420197No Hit
GTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTC869890.4016635220593335No Hit
CTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTT840970.3883099841890787No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG840250.3879775309640931No Hit
GTATAATTACCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCCTC824560.3807328211029487No Hit
ACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGT806490.37238916863699073No Hit
GTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTA722830.3337599508560255No Hit
GTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCA681370.31461618598393826No Hit
ATTTAGAACCAGCCTCATCCACGCACAATTCAATTAAAGGTGCTGATTTT667500.308211843997063No Hit
ACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGATGATTCCT655160.30251396511328205No Hit
AAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCT651060.3006208286932252No Hit
CTGTATAATTACCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCC619050.28584051239907393No Hit
CTCCTACTCTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCA614210.2836056879422263No Hit
ATTCAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAATATACCATTTA608250.28085371402428994No Hit
CACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATG604140.2789559601983305No Hit
ACTACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTG581330.26842365733455076No Hit
CTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTA572640.2644111316052107No Hit
GTTGTGATGATTCCTAAGAAAACAAGAAATTTCATGTTCGTTTAGGCGTG563540.26020929223386496No Hit
CAATTCAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAATATACCATT557780.2575496664339802No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC549220.2535971669813737No Hit
CTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACTACCCAATTTA547330.2527244772657865No Hit
CGTCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGA539060.2489058825843547No Hit
AATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACAT537560.24821327169896804No Hit
ATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACATATG531200.24527660154492864No Hit
GCAATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATG523770.24184586895931334No Hit
GACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCG510370.2356585450498592No Hit
GCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTT495370.22873243619599262No Hit
ACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAACAGGAAACTG491250.2268300649641306No Hit
ATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTG490640.22654840320407338No Hit
ATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAACAGGAAACTGTATAA488390.22550948687599337No Hit
GTGTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGT481730.2224342945448766No Hit
ATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGC474320.2190127967710665No Hit
ATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTAC458140.21154183402069576No Hit
TAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAG457490.21124170263702818No Hit
GTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTT455630.21038286513914875No Hit
GTACTGAATGGGTGATTTAGAACCAGCCTCATCCACGCACAATTCAATTA453800.20953787985897704No Hit
GAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTTATAAC453390.20934856621697134No Hit
ATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAA446660.20624105204453655No Hit
GAATAGCAGAAAGGCTAAAAAGCACAAATAGAAGTCAATTAATGAAAGTT446130.20599632953169994No Hit
TAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAAT445110.20552535412963702No Hit
ACAGGAAACTGTATAATTACCGATATCGATGTACTGAATGGGTGATTTAG442920.20451414223697245No Hit
TTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTG440820.20354448699743113No Hit
CTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTG440650.20346599109708732No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC438910.2026625624700388No Hit
GGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAAACTACAT438050.20226546556241712No Hit
GTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGT432150.1995411960798963No Hit
CTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTTATAACACTTTGCTTC427650.1974633634237363No Hit
GTTTAGATGAAATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTC425750.19658605630224651No Hit
GAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTAC425230.19634595119531248No Hit
GTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGTTC419560.1937278820485509No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC413750.19104516921915327No Hit
CAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAATATACCATTTAGAA411980.190227888374397No Hit
GGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAA410440.1895168078654No Hit
GTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAA404980.1869957042425926No Hit
ATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAG395250.18250296829938448No Hit
TCCACGCACAATTCAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAAT390610.18036049196058843No Hit
ATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGTCACGC388040.17917381864362594No Hit
GGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAATGCAGC385220.177871710179099No Hit
CTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCT384930.17773780540792428No Hit
CATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTACAGTCATGTA384390.17748846548918507No Hit
ACGTAATGCGGGGTGCATTTCGCTGATTTTGGGGTCCATTATCAGACATT383030.1768604982864345No Hit
AATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAATATACCATTTAGAAT382710.17671274129755202No Hit
GTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCC372950.17220615313663615No Hit
GATTTTGGGGTCCATTATCAGACATTTTAGTTTGTTCGTTTAGATGAAAT363600.167888878617726No Hit
GCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACT363510.16784732196460278No Hit
ATTTTGGGGTCCATTATCAGACATTTTAGTTTGTTCGTTTAGATGAAATC357670.1651507569174974No Hit
GTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTC357220.1649429736518814No Hit
GTTCAAGTGAGAACCAAAAGATAATAAGCATAATTAAAACAAGGAATAGC356410.16456896377377261No Hit
GTTGAGTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTT347480.16044562030277068No Hit
ATCATAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTC335650.15498322912002124No Hit
ACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTTATAACAC329600.15218969854896172No Hit
GAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTT326910.15094761636116832No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC325110.1501164832987043No Hit
ACTCTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAAC323690.1494608116605383No Hit
CAACTACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGG323680.14945619425463572No Hit
CTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTG321740.14856041750953564No Hit
ATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCT315760.14579920877979416No Hit
GTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGATGATTCCTAAG314660.14529129413051062No Hit
ATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAACACGAACGTCATGA313500.1447556750458116No Hit
GACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAAC301290.1391178224387642No Hit
TTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTT300860.13891927398495335No Hit
GTCCATTATCAGACATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAA296670.1369845809117733No Hit
ATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATA291860.1347636086726334No Hit
GCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGTTTCACCTAAACTG291620.13465279093097154No Hit
GGTAAACAGGAAACTGTATAATTACCGATATCGATGTACTGAATGGGTGA290470.1341217892521751No Hit
ACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTA290330.13405714556953902No Hit
CCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTG288180.1330644033004848No Hit
GAGTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTG287000.13251954940398064No Hit
CTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTT284400.13131902386931044No Hit
CATTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTT283270.13079725700231914No Hit
CTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGC281030.12976295808014174No Hit
CGGCAATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAA274800.1268863142028358No Hit
GATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAG270570.12493315150604543No Hit
GTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACA269010.12421283618524331No Hit
AGAATGTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATG268940.12418051434392526No Hit
CCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCA268810.12412048806719174No Hit
ACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTA263920.12186257658083124No Hit
GTTTAGGTGAAACTGATCTGGCACGTAACTGATAGACGTGTTTTACGCCG263510.12167326293882556No Hit
ACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTA261660.12081904284684869No Hit
TGCCAGATCAGTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAG259290.11972471764793775No Hit
CACTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTA258970.11957696065905526No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT258100.119175246345531No Hit
ATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATT255960.11818712148237939No Hit
TCGATGTACTGAATGGGTGATTTAGAACCAGCCTCATCCACGCACAATTC254670.11759147612094686No Hit
TTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTG254340.11743910172616179No Hit
ATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAATATACCATTTAGAATA252250.11647406389252304No Hit
GTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGT252090.11640018539808179No Hit
AAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATT249890.1153843560995147No Hit
CTACATTCTTGGTGAAATGCAGCTACAGTTGTGATGATTCCTAAGAAAAC249470.11519042505160643No Hit
GTCTTCATAGAACGAACAACGCACTACAAGACTACCCAATTTAGGTTCCT249340.11513039877487291No Hit
CAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAACAGGAAACTGTAT248670.11482103257940023No Hit
CTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGATGATTCCTA246140.11365282888604807No Hit
CTACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGA245550.11338040193779597No Hit
GGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC245540.1133757845318934No Hit
GATCAGTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTT245240.11323726235481606No Hit
ACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACTACCCAAT244280.11279399138816859No Hit
GAATGTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGA244220.11276628695275313No Hit
GCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAAC243460.11241536410415721No Hit
TGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCTG242860.11213831975000256No Hit
TTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGG239500.11058687136673645No Hit
GTAAAAGGTAAACAGGAAACTGTATAATTACCGATATCGATGTACTGAAT238660.11019900927091993No Hit
CCACTGCGTTCTCCATTCTGGTTACTGCCAGTTGAATCTGAGGGTCCACC236420.1091647103487425No Hit
AGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTG235800.1088784311827827No Hit
GAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAG235580.10877684825292598No Hit
GTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTC234240.10815811586198057No Hit
CCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTA234100.10809347217934448No Hit
GGTTTATGATGTAATCAAGATTCCAAATGGAAACTTTAAAAGTCCTCATA233640.10788107150782592No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG232420.10731774798771142No Hit
ATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGAT232340.1072808087404908No Hit
GTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTA231090.10670363300266858No Hit
GTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCG228560.10553542930931642No Hit
GACTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTAT227870.10521682830203856No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC227640.10511062796627928No Hit
AAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTT226440.10455653925796994No Hit
CATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAACACGAACGTCATG224340.10358688401842862No Hit
CTGCTATAGTAACCTGAAAGTCAACGAGATGAAACATCTGTTGTCACTTA221900.10246023697819966No Hit
CTCTTGGTAGTGATAAAGCTCACAAGTAGCGAGTGTTATCAGTGCCAAGA220770.10193847011120837No Hit
ATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAA218480.10088108415951806No Hit
CAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCA218440.10086261453590778No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG217730.10053477871682474No Hit
GTTACGTGCCAGATCAGTTTCACCTAAACTGTTCATCAGACAAGAGGAAG216620.10002224666163861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA140450.0137.921591
TCCTAGG163450.0118.7146153
CCTAGGT166800.0116.438734
TTCCTAG175250.0110.482432
CTAGGTA176650.0110.31195
TAGGTAG183000.0107.128116
AGGTAGT219300.089.467467
CAGGTAT6600.076.8908844
GCAGGTA8050.063.0529173
CGTCTTC18100.057.2748875
CAGACTG62050.054.79465
TTGTAGA240650.054.562642
TATGATA35700.054.01709145
GTCGTCT22550.053.6998333
TTCAGAC63500.053.555263
TTCACTA36550.053.21731
TCTGTTC250050.052.7790579
AAATTAG61750.052.3606035
GCCGACC3750.052.197567145
CGGACTG1950.052.0475355
Produced by FastQC (version 0.11.5)