FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-42_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-42_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7819787
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATGACCCTGCTGGGGCAGAAGATCCCCTGGGAGCAATTCACTTGAGA1029981.3171458506478502No Hit
CTCTTGGACATGTCTCCAGCAGGCTGCAGATACCCCTCATTGAGCAGCGA715180.9145773407894613No Hit
TTCCTGGACAACCCTGATGCCTTCTACTACTTTCCAGACAGTGGGTTCTT599190.7662484924461498No Hit
TTTCCAGACAGTGGGTTCTTCTGTGAAGAGAATTCCAGTGATGATGATGT506630.6478820970443312No Hit
GTCCAGGAGTGCAGTGGATGGAACTGCTGAAAACCCTTTCCTGGACAACC458390.5861924372108857No Hit
TTCTACTACTTTCCAGACAGTGGGTTCTTCTGTGAAGAGAATTCCAGTGA448480.5735194577550514No Hit
GGATGGAACTGCTGAAAACCCTTTCCTGGACAACCCTGATGCCTTCTACT447450.5722022863282593No Hit
GGTCATAGTAGTGCAGGTAGGCAGGGTCTTCTCTCAAGATGAACTTCCTC401480.5134155188625982No Hit
GGAGTGCAGTGGATGGAACTGCTGAAAACCCTTTCCTGGACAACCCTGAT380030.4859851041978509No Hit
GTCCAGGAAAGGGTTTTCAGCAGTTCCATCCACTGCACTCTTGGACATGT379000.48466793277105885No Hit
GGACAACCCTGATGCCTTCTACTACTTTCCAGACAGTGGGTTCTTCTGTG330320.42241559776500304No Hit
CCAATATTCCAGGCACCACCTTTCATCCAGCCCATCTGCAGGACAGGACG326710.4177991037351785No Hit
TCATTGAGCAGCGATGAAGCAATCATGAGGCCTTCCTGGCGATTCCTAAC320820.4102669292654647No Hit
GGAAAGGGTTTTCAGCAGTTCCATCCACTGCACTCTTGGACATGTCTCCA312650.3998190743558616No Hit
GGTTGGATACCAGCCAATCAATGACGCAGTTACCATTTGAGTTGCTCTCC311060.397785770891202No Hit
CAATGTGTCCAGGGTGACATTACGTCCTGTCCTGCAGATGGGCTGGATGA303990.3887446039131245No Hit
ACCCTGATGCCTTCTACTACTTTCCAGACAGTGGGTTCTTCTGTGAAGAG300550.3843455071090811No Hit
ATTCCTAACAGACTGGTTGGATACCAGCCAATCAATGACGCAGTTACCAT298840.38215874677916417No Hit
GTTCCATCCACTGCACTCTTGGACATGTCTCCAGCAGGCTGCAGATACCC298520.3817495284718113No Hit
TTATCAAGCAGGGATGTTTACTGAAGCAGGGGCATAGAAGGAAAAACTGG287390.3675164042191942No Hit
GTCCAGGGTGACATTACGTCCTGTCCTGCAGATGGGCTGGATGAAAGGTG277490.3548562128354647No Hit
CCACTGAAGTCACCACACAGCCTCTCAAGTGAATTGCTCCCAGGGGATCT275000.3516719828813752No Hit
CTTCAGTGGAGAGCAACTCAAATGGTAACTGCGTCATTGATTGGCTGGTA270180.34550813212687254No Hit
CTTGGACATGTCTCCAGCAGGCTGCAGATACCCCTCATTGAGCAGCGATG261190.33401165530467775No Hit
TAGTAGTGCAGGTAGGCAGGGTCTTCTCTCAAGATGAACTTCCTCACTTT257290.3290243071838146No Hit
GTGTCCAGGAGTGCAGTGGATGGAACTGCTGAAAACCCTTTCCTGGACAA253800.32456127001924734No Hit
TCCATCCACTGCACTCTTGGACATGTCTCCAGCAGGCTGCAGATACCCCT248190.3173871615684673No Hit
GGTCATTATCAAGCAGGGATGTTTACTGAAGCAGGGGCATAGAAGGAAAA247380.3163513277279803No Hit
GTTGGATACCAGCCAATCAATGACGCAGTTACCATTTGAGTTGCTCTCCA246800.3156096195459032No Hit
AGTCACCACACAGCCTCTCAAGTGAATTGCTCCCAGGGGATCTTCTGCCC238510.3050083077710429No Hit
GCTGAAAACCCTTTCCTGGACAACCCTGATGCCTTCTACTACTTTCCAGA237140.3032563418926884No Hit
GGGTCATAGTAGTGCAGGTAGGCAGGGTCTTCTCTCAAGATGAACTTCCT234560.29995701928965585No Hit
GGCTCAATGTGTCCAGGGTGACATTACGTCCTGTCCTGCAGATGGGCTGG233850.29904906617021665No Hit
CCTAACAGACTGGTTGGATACCAGCCAATCAATGACGCAGTTACCATTTG226250.2893301313705859No Hit
GCAGATACCCCTCATTGAGCAGCGATGAAGCAATCATGAGGCCTTCCTGG224080.2865551197238492No Hit
GCACTACTATGACCCTGCTGGGGCAGAAGATCCCCTGGGAGCAATTCACT220850.2824245724340062No Hit
GCGATGAAGCAATCATGAGGCCTTCCTGGCGATTCCTAACAGACTGGTTG219900.28120970558405234No Hit
GTTCACCAATATTCCAGGCACCACCTTTCATCCAGCCCATCTGCAGGACA217250.27782086647628634No Hit
GTCTCCAGCAGGCTGCAGATACCCCTCATTGAGCAGCGATGAAGCAATCA214640.2744831796569395No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCACCTTTCATCCAGCCC205870.26326804042104984No Hit
GTGGATGGAACTGCTGAAAACCCTTTCCTGGACAACCCTGATGCCTTCTA205340.26259027259949663No Hit
ATCATGAGGCCTTCCTGGCGATTCCTAACAGACTGGTTGGATACCAGCCA203920.26077436636061824No Hit
CTATGACCCTGCTGGGGCAGAAGATCCCCTGGGAGCAATTCACTTGAGAG200240.2560683558260602No Hit
CCCTGATGCCTTCTACTACTTTCCAGACAGTGGGTTCTTCTGTGAAGAGA198420.25374092670299075No Hit
GTTTATGGAGTGTGGGAGGGAGGTGTCAGGAGGATGGGGGTGAGGAGGTT193070.2468993081269349No Hit
GTGAGGAAGTTCATCTTGAGAGAAGACCCTGCCTACCTGCACTACTATGA192350.24597856693539094No Hit
ATAATGACCCCTCTGAATTCTTCTTTCAGAATCACATCATCATCACTGGA182030.2327812765232608No Hit
GTTTTCAGCAGTTCCATCCACTGCACTCTTGGACATGTCTCCAGCAGGCT180220.2304666354722961No Hit
GTATCCAACCAGTCTGTTAGGAATCGCCAGGAAGGCCTCATGATTGCTTC179200.22916225211760885No Hit
CTCCAGCAGGCTGCAGATACCCCTCATTGAGCAGCGATGAAGCAATCATG178130.22779392840239768No Hit
GGGTTCTTCTGTGAAGAGAATTCCAGTGATGATGATGTGATTCTGAAAGA177770.22733355780662567No Hit
AATGTGTCCAGGGTGACATTACGTCCTGTCCTGCAGATGGGCTGGATGAA173270.2215789253594759No Hit
CATGAGGCCTTCCTGGCGATTCCTAACAGACTGGTTGGATACCAGCCAAT172830.22101625018686571No Hit
GCCTCTCAAGTGAATTGCTCCCAGGGGATCTTCTGCCCCAGCAGGGTCAT167670.21441760498080065No Hit
GTGGCGACTTCAGTGGAGAGCAACTCAAATGGTAACTGCGTCATTGATTG165710.2119111428482643No Hit
CATTACGTCCTGTCCTGCAGATGGGCTGGATGAAAGGTGGTGCCTGGAAT165680.21187277863194995No Hit
GTCACCACACAGCCTCTCAAGTGAATTGCTCCCAGGGGATCTTCTGCCCC154880.1980616607587905No Hit
GTTTAGCACGCCTCTCTTCAAACAGTTCTCTCCTTTCATTTTCAACCTGC153080.19575980777993057No Hit
GCCTTCTACTACTTTCCAGACAGTGGGTTCTTCTGTGAAGAGAATTCCAG149760.19151416784114453No Hit
GTTCTAGAAAGTGCTTTCCAAAGTTTTATTTTTTTATTTGTACCTGCCTT144750.1851073437166511No Hit
ATTCAGAGGGGTCATTATCAAGCAGGGATGTTTACTGAAGCAGGGGCATA141120.18046527354261696No Hit
CAAGTGAATTGCTCCCAGGGGATCTTCTGCCCCAGCAGGGTCATAGTAGT140760.18000490294684499No Hit
GAATTCCAGTGATGATGATGTGATTCTGAAAGAAGAATTCAGAGGGGTCA140040.179084161755301No Hit
ACTTTCCAGACAGTGGGTTCTTCTGTGAAGAGAATTCCAGTGATGATGAT136190.17416075399496175No Hit
GTGGGTTCTTCTGTGAAGAGAATTCCAGTGATGATGATGTGATTCTGAAA135370.17311213208237003No Hit
GATGTTTACTGAAGCAGGGGCATAGAAGGAAAAACTGGAAAGTGAGGAAG133910.1712450735550725No Hit
CTACTACTTTCCAGACAGTGGGTTCTTCTGTGAAGAGAATTCCAGTGATG133840.17115555705033908No Hit
CTTCTGTGAAGAGAATTCCAGTGATGATGATGTGATTCTGAAAGAAGAAT128880.16481267328636956No Hit
CCACTGCACTCTTGGACATGTCTCCAGCAGGCTGCAGATACCCCTCATTG124290.1589429481902768No Hit
CAGCAGTTCCATCCACTGCACTCTTGGACATGTCTCCAGCAGGCTGCAGA124020.15859767024344779No Hit
CCATTTGAGTTGCTCTCCACTGAAGTCACCACACAGCCTCTCAAGTGAAT123050.15735722724928441No Hit
GCTTGATAATGACCCCTCTGAATTCTTCTTTCAGAATCACATCATCATCA122400.15652600256247387No Hit
GGGTGACATTACGTCCTGTCCTGCAGATGGGCTGGATGAAAGGTGGTGCC122160.15621908883195923No Hit
GGCTCAATGTGTCCAGGAAAGGGTTTTCAGCAGTTCCATCCACTGCACTC121910.1558993870293398No Hit
GTCATAGTAGTGCAGGTAGGCAGGGTCTTCTCTCAAGATGAACTTCCTCA121880.15586102281302547No Hit
CTTGAGAGGCTGTGTGGTGACTTCAGTGGAGAGCAACTCAAATGGTAACT121490.15536228800093915No Hit
CAATGTGTCCAGGAAAGGGTTTTCAGCAGTTCCATCCACTGCACTCTTGG121300.15511931463094839No Hit
TCTGTGAAGAGAATTCCAGTGATGATGATGTGATTCTGAAAGAAGAATTC120570.15418578536729965No Hit
CCACTGAAGTCGCCACACAGCCTCTCAAGTGAATTGCTCCCAGGGGATCT118850.15198623696527797No Hit
GAGAAGACCCTGCCTACCTGCACTACTATGACCCTGCTGGGGCAGAAGAT118150.15109107191794355No Hit
GCGCATCTGTTCACCAATATTCCAGGCACCACCTTTCATCCAGCCCATCT117000.14962044362589416No Hit
CCACACAGCCTCTCAAGTGAATTGCTCCCAGGGGATCTTCTGCCCCAGCA116560.14905776845328395No Hit
GTAAACATCCCTGCTTGATAATGACCCCTCTGAATTCTTCTTTCAGAATC116060.1484183648480451No Hit
GGGATGTTTACTGAAGCAGGGGCATAGAAGGAAAAACTGGAAAGTGAGGA114000.14578402199446097No Hit
GTGAAGAGAATTCCAGTGATGATGATGTGATTCTGAAAGAAGAATTCAGA112190.1434693809434963No Hit
CAATGTGTCCAGGAGTGCAGTGGATGGAACTGCTGAAAACCCTTTCCTGG110450.14124425639726504No Hit
GTAGTGCAGGTAGGCAGGGTCTTCTCTCAAGATGAACTTCCTCACTTTCC109380.1398759326820539No Hit
CCCCTCATTGAGCAGCGATGAAGCAATCATGAGGCCTTCCTGGCGATTCC109130.13955623087943445No Hit
TCCTAACAGACTGGTTGGATACCAGCCAATCAATGACGCAGTTACCATTT108980.1393644097978628No Hit
GGCTCAATGTGTCCAGGAGTGCAGTGGATGGAACTGCTGAAAACCCTTTC108600.13887846305788124No Hit
CTAACAGACTGGTTGGATACCAGCCAATCAATGACGCAGTTACCATTTGA108270.1384564566784236No Hit
CATGAAGACAGTGTTCTCAAAAGAAGAGAGTGGTCAACCACATCAAGGGC107100.13696025224216465No Hit
GTTACCATTTGAGTTGCTCTCCACTGAAGTCACCACACAGCCTCTCAAGT106020.1355791404548487No Hit
TTGCGCATCTGTTCACCAATATTCCAGGCACCACCTTTCATCCAGCCCAT105190.1345177304701522No Hit
GAAGAATTCAGAGGGGTCATTATCAAGCAGGGATGTTTACTGAAGCAGGG104590.13375044614386555No Hit
GGTTTTCAGCAGTTCCATCCACTGCACTCTTGGACATGTCTCCAGCAGGC103710.13262509579864518No Hit
CCCTAGCATTGTTCGTTACATGGTCCATCATAGAATTCTCACTGTGATAT103460.13230539399602573No Hit
AATGGTAACTGCGTCATTGATTGGCTGGTATCCAACCAGTCTGTTAGGAA103410.13224145363550183No Hit
CAATAGTACCAAGCTCAAAGGTTGGTACTCAAAACCTGTGGGGATTAAAT102900.1315892619581582No Hit
TTACTGTTCTGTCCCCACTCTCTTATCTCCCACTCACTCCTCAATCCACT102210.13070688498292857No Hit
GTCTGTTAGGAATCGCCAGGAAGGCCTCATGATTGCTTCATCGCTGCTCA100200.12813648248986834No Hit
GCACTCTTGGACATGTCTCCAGCAGGCTGCAGATACCCCTCATTGAGCAG100180.12811090634565878No Hit
GTGTGGCGACTTCAGTGGAGAGCAACTCAAATGGTAACTGCGTCATTGAT99170.12681931106307628No Hit
GTCTTCTCTCAAGATGAACTTCCTCACTTTCCAGTTTTTCCTTCTATGCC98870.12643566889993296No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC98600.12609039095310398No Hit
GTTCTTCTGTGAAGAGAATTCCAGTGATGATGATGTGATTCTGAAAGAAG98070.12541262313155077No Hit
GTTAGGAATCGCCAGGAAGGCCTCATGATTGCTTCATCGCTGCTCAATGA97630.12484994795894057No Hit
GTGTGGTGACTTCAGTGGAGAGCAACTCAAATGGTAACTGCGTCATTGAT97180.12427448471422559No Hit
GAATTCAGAGGGGTCATTATCAAGCAGGGATGTTTACTGAAGCAGGGGCA95900.12263761148481409No Hit
TCTGTTCACCAATATTCCAGGCACCACCTTTCATCCAGCCCATCTGCAGG95740.12243300233113767No Hit
AATATAATGAGGATGAACGTTTATTGAATGAATGAACATTTACTGGCTAT94770.12119255933697426No Hit
CTACTATGACCCTGCTGGGGCAGAAGATCCCCTGGGAGCAATTCACTTGA94310.12060430802015452No Hit
GTTTACTGAAGCAGGGGCATAGAAGGAAAAACTGGAAAGTGAGGAAGTTC93940.12013114935227776No Hit
GTCCTAGGCTAGAGGGGGTATCCCTAGTTAGACTTTGGGAAGCCACCTTC93800.11995211634281087No Hit
CTTCCTCACTTTCCAGTTTTTCCTTCTATGCCCCTGCTTCAGTAAACATC93140.11910810358389558No Hit
ATCCACTGCACTCTTGGACATGTCTCCAGCAGGCTGCAGATACCCCTCAT92580.11839197154602805No Hit
GTTCTTACTAAACTACTATCTGCATCATTATTACAAAATCCAAGAAATCC92450.11822572660866594No Hit
ATGTGATTCTGAAAGAAGAATTCAGAGGGGTCATTATCAAGCAGGGATGT91420.11690855518187387No Hit
CTGTTCACCAATATTCCAGGCACCACCTTTCATCCAGCCCATCTGCAGGA91200.11662721759556878No Hit
GCCTACCTGCACTACTATGACCCTGCTGGGGCAGAAGATCCCCTGGGAGC90990.11635866808136847No Hit
CTTCTACTACTTTCCAGACAGTGGGTTCTTCTGTGAAGAGAATTCCAGTG90780.11609011856716814No Hit
CTTCTGCCCCAGCAGGGTCATAGTAGTGCAGGTAGGCAGGGTCTTCTCTC90760.11606454242295858No Hit
TGTCTCCAGCAGGCTGCAGATACCCCTCATTGAGCAGCGATGAAGCAATC89290.11418469582355631No Hit
GCTCCCAGGGGATCTTCTGCCCCAGCAGGGTCATAGTAGTGCAGGTAGGC88240.11284194825255471No Hit
GTGGAGAGCAACTCAAATGGTAACTGCGTCATTGATTGGCTGGTATCCAA87630.11206187585416329No Hit
GAACTGCTGAAAACCCTTTCCTGGACAACCCTGATGCCTTCTACTACTTT86660.11082143285999989No Hit
TTCCAGTGATGATGATGTGATTCTGAAAGAAGAATTCAGAGGGGTCATTA85320.10910783119795975No Hit
ATTCCAGGCACCACCTTTCATCCAGCCCATCTGCAGGACAGGACGTAATG84410.107944116636425No Hit
GGGGATCTTCTGCCCCAGCAGGGTCATAGTAGTGCAGGTAGGCAGGGTCT83830.10720240845434792No Hit
GAATTGCTCCCAGGGGATCTTCTGCCCCAGCAGGGTCATAGTAGTGCAGG83780.10713846809382403No Hit
ATTCACTTGAGAGGCTGTGTGGTGACTTCAGTGGAGAGCAACTCAAATGG83560.10685713050751894No Hit
GCTCTCCACTGAAGTCACCACACAGCCTCTCAAGTGAATTGCTCCCAGGG83530.10681876629120461No Hit
ATGATGATGTGATTCTGAAAGAAGAATTCAGAGGGGTCATTATCAAGCAG83430.10669088557015682No Hit
GGTAGGCAGGGTCTTCTCTCAAGATGAACTTCCTCACTTTCCAGTTTTTC83340.10657579292121383No Hit
CCCTCATTGAGCAGCGATGAAGCAATCATGAGGCCTTCCTGGCGATTCCT83340.10657579292121383No Hit
TCAGTAAACATCCCTGCTTGATAATGACCCCTCTGAATTCTTCTTTCAGA82270.10520746920600267No Hit
ATATTCCAGGCACCACCTTTCATCCAGCCCATCTGCAGGACAGGACGTAA81310.10397981428394405No Hit
CATCTGTTCACCAATATTCCAGGCACCACCTTTCATCCAGCCCATCTGCA81140.10376241705816285No Hit
ATATAAACTCAGACCCAAACATTAATCAGTTCTTCAAATATCTACTCATT80750.10326368224607653No Hit
ATCAAGCAGGGATGTTTACTGAAGCAGGGGCATAGAAGGAAAAACTGGAA80570.10303349694819053No Hit
GTGTCCAGGAAAGGGTTTTCAGCAGTTCCATCCACTGCACTCTTGGACAT80330.10272658321767587No Hit
GGTTTTACCTTCTTCAGTTCTAGAAAGTGCTTTCCAAAGTTTTATTTTTT80320.10271379514557111No Hit
CCTTTCATCCAGCCCATCTGCAGGACAGGACGTAATGTCACCCTGGACAC80290.10267543092925677No Hit
GTTCATCTTGAGAGAAGACCCTGCCTACCTGCACTACTATGACCCTGCTG79810.10206160346822746No Hit
ATTACGTCCTGTCCTGCAGATGGGCTGGATGAAAGGTGGTGCCTGGAATA79600.10179305395402714No Hit
CAGGAGTGCAGTGGATGGAACTGCTGAAAACCCTTTCCTGGACAACCCTG79370.10149892829561726No Hit
TAGTTAGACTTTGGGAAGCCACCTTCAATGTTTTCTGGAACAAGGCAGGT79270.1013710475745695No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA109250.096.269441
GTTTGCG44700.095.3173751
CCCTAGC133400.092.3896561
TAGCACG27800.089.970824
TTTGCGC48550.087.414392
CTCCCTA53100.086.800191
CGGACAA4400.085.669315
CCTAGCA146650.084.691152
TTGTTCG23550.083.750219
AGCACGC30300.081.819735
CTAGCAT152900.080.426383
GACGAGG4650.079.608371
ACGCCTC31050.078.930778
GCATTGT44350.078.627286
GCACGCC31200.077.8416446
TCCCGGA5500.077.822582
CACGCCT31750.076.72947
CGAGGTC5100.072.5070343
TGCGCAT58550.072.064844
AGCATTG171950.071.62525
Produced by FastQC (version 0.11.5)