FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-41_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-41_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7698065
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT853701.1089799839310268No Hit
CCCTAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAG793621.0309343971504528No Hit
CCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTT784931.0196458460665117No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCC692880.9000703423522664No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC506760.6582952988835507No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC456390.5928632714844575No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA412120.5353553133157488No Hit
CATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAG399600.5190914859773202No Hit
TTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAA384670.4996970017790185No Hit
GTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACCA377830.4908116520190463No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA376540.4891359062309814No Hit
GTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCAT367100.47687308434002573No Hit
CCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGG352400.4577773765225417No Hit
CCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCT328680.42696443846603016No Hit
GCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTA300940.39092940888392086No Hit
GTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAAC299240.3887210617213547No Hit
GGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTA285690.3711192358079595No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT284070.3690148108648082No Hit
CTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCC283240.36793661783837883No Hit
CAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGA273990.35592061121853347No Hit
CTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACT272960.35458261264356694No Hit
GCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTT261620.3398516380415078No Hit
GCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTAC251660.3269133217243554No Hit
CCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACC251350.3265106231241227No Hit
GGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACA250780.3257701773107918No Hit
TTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGAATCACCATTAGC246700.3204701441206329No Hit
GTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTT235470.3058820625702693No Hit
GGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAA235450.3058560820154156No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT230080.2988803030371918No Hit
CTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTATACATTTG226100.2937101726213016No Hit
CCCTAGCATTGTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTA218550.2839025131640224No Hit
CTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGC210090.2729127384608989No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG201360.2615722262672503No Hit
CATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGTTGCATGG199500.25915603466585435No Hit
ATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTG199310.258909219394744No Hit
GCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGT195510.25397291397253724No Hit
GACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTC181800.23616324362031238No Hit
CTCCCTAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTC178920.23242204372137676No Hit
ACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGC178250.23155169513377713No Hit
TAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCT174250.2263555841630332No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG171570.22287418981263474No Hit
GGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATA171460.22273129676093928No Hit
ATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCAC169950.2207697648694834No Hit
GTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGA169240.21984745517217638No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT164420.2135861414524299No Hit
GTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTT163110.21188441510951128No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA163010.2117545123352427No Hit
GCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTATACAT161920.21033857209571497No Hit
GTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAA158510.20590888749315575No Hit
ATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTT156570.20338877367234495No Hit
ATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCA155950.20258337647187963No Hit
GTAGCAAAAGCTGCATATGATGGAAGGGAACTAAACTCTGAGGCTATAGC153570.19949169044428697No Hit
ACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGTAGACATAAACAAGCTTT153080.19885516685037083No Hit
GTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAAC152790.19847844880499188No Hit
ATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGC150080.19495808362231287No Hit
CCCTTGGAGAGTGCTAGTTGCCATCTCTTTTTGAGGGTTATGATTTTGGA148260.19259385313062438No Hit
GCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAAC147510.1916195823236099No Hit
TGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCT147380.1914507087170607No Hit
CACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAAT147350.19141173788478014No Hit
AGTAAATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTA146330.19008672958724043No Hit
TGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGA145830.18943721571589742No Hit
TTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAA143870.1868911213402329No Hit
GTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCC138010.17927881876809304No Hit
TCTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCG137470.1785773437870426No Hit
AACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCG134970.1753297744303276No Hit
ACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGC134150.17426457168132511No Hit
GCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAA133320.17318637865489575No Hit
GCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGA133300.17316039810004202No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC131310.17057533289209692No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAAC130710.16979591624648532No Hit
GGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGT130700.16978292596905847No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG130350.16932826625911837No Hit
GTCCATACTAATTTCACTAAGTTGAACAATTTTACTATCTGCATCTACAA129410.16810718018099355No Hit
CTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAG128640.16710692881912534No Hit
CCATTAGCAACAGCCTGCTCATAAGCTTCTTGAGCAGTAGCAAAAGCTGC127110.16511941637281577No Hit
TTGCATGGCTGCATCACGGTCAAATTCAGATTTAGCCACATTCAAAGACT127090.16509343581796204No Hit
CTTGTCCTCAGATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAG126070.16376842752042234No Hit
GGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGTTGCATGGCTGCAT125260.1627162150488467No Hit
GTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGT124980.1623524872808946No Hit
CCCTAGCATTGTCATCAGTGCAAGCAGTTTGTGTAGTACCGGCAGCACAA123150.15997526651177926No Hit
GAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGC121040.15723431797471182No Hit
GTTGTAAGAGGTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTT119270.15493503887015764No Hit
ATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGAAAAGCAT117480.15260977921074972No Hit
GTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGGTA117050.15205119728139474No Hit
GCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGT116010.15070020842900134No Hit
ATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTT115520.1500636848350852No Hit
CAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGG114990.1493752001314616No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC114350.14854382237614258No Hit
GACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACA113280.14715386269146857No Hit
CCTCAGATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATC111790.14521831135486646No Hit
GATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAA109540.14229549893382298No Hit
GTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTTGATAATGTTG109430.14215260588212753No Hit
GAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTG109360.14206167394013952No Hit
GTGTAGTACCGGCAGCACAAGACATCTGTCGTAGTGCAACAGGACTAAGC107380.13948959900962124No Hit
CTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGC105930.13760600878272658No Hit
CTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTATACATTTGGGTCATA105660.13725527129220136No Hit
ACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCAC102960.13374789638694917No Hit
GTCATAGCTTGATCAGCCATCTTTTCCAACTTACGTTGCATGGCTGCATC102680.1333841686189971No Hit
TTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAAT101880.13234494642484831No Hit
GACTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGC101230.13150057839210244No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG100370.13038341453339247No Hit
GTATAGATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAA99490.12924027011982883No Hit
AGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCT98710.12822702848053374No Hit
TTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCA98580.12805815487398456No Hit
AGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTA98330.12773339793831306No Hit
ATTTTACTATCTGCATCTACAACCTGTTGGATTTCCCACAATGCTGATGC98260.12764246599632503No Hit
CATTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGAATCACCATTA97500.1266552049118837No Hit
GTGAAAAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTC96150.12490151745925761No Hit
GTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTG95640.12423901331048777No Hit
GCTCATAAGCTTCTTGAGCAGTAGCAAAAGCTGCATATGATGGAAGGGAA95530.12409612025879231No Hit
GTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCT95520.12408312998136546No Hit
CACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATC94940.12332969389060758No Hit
GTGCAAGCAGTTTGTGTAGTACCGGCAGCACAAGACATCTGTCGTAGTGC93960.1220566467027753No Hit
GCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAG93730.12175787032195752No Hit
CATCTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTG93140.12099144395377279No Hit
CTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGC92360.11997820231447773No Hit
ATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTC92320.11992624120477027No Hit
AATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAA92200.11977035787564796No Hit
TCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAAT91040.11826348569413223No Hit
GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG90480.11753603015822807No Hit
ATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTATGACA89990.11689950656431193No Hit
CTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTT89280.11597719686700489No Hit
GCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCA88960.11556150798934536No Hit
GCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTA88260.11465218856946519No Hit
GCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCC87850.11411958719496393No Hit
ATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTG86920.11291149139426597No Hit
GCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAA86350.11217104558093494No Hit
GTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGAT85940.11163844420643369No Hit
GCAACAGCCTGCTCATAAGCTTCTTGAGCAGTAGCAAAAGCTGCATATGA85440.1109889303350907No Hit
ATGCAGGGTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAA85150.11061221228971176No Hit
AGCTTGATCAGCCATCTTTTCCAACTTACGTTGCATGGCTGCATCACGGT85030.11045632896058945No Hit
GCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTA84680.11000166925064934No Hit
GTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGAAAAGCATTG84360.10958598037298985No Hit
ATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTA84260.10945607759872122No Hit
CTAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGAT83480.10844283595942616No Hit
CCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGT82950.1077543512558026No Hit
GCTCATTATTCTGTAATTTGACAGCAGAATTGGCCCTTAAAGCTGTTACA81920.10641635268083603No Hit
CTGTTTTGCTTTCCATGCAGGGTGCTGTAGACATAAACAAGCTTTGTGAA81270.10557198464809013No Hit
GATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCT81190.10546806242867526No Hit
GAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACCAATGTG80650.10476658744762483No Hit
GTACCAGTGTGTGTACACAACATCTTAACACAATTAGTGATTGGTTGTCC79640.10345456942751198No Hit
GTACAACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTA78980.10259721111733923No Hit
CTCTTAGGGAATCTAGCCCATTTCAAATCCTGTAAATCGGATAACAGTGC78620.10212956112997226No Hit
CACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTG78580.10207760002026484No Hit
CAATAAGAGGCCATGCTAAATTAGGTGAATTGTCCATACTAATTTCACTA78480.10194769724599624No Hit
TCATTATCCAACTTTCTAAGCATAGTGAAAAGCATTGTCTGCATAGCACT78160.10153200836833672No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGC190750.0110.328431
TCCCTAG45300.0109.535932
CTCCCTA46100.0107.516171
CCTAGCA202250.0104.4483262
TCAATGT56300.0103.140524
GCTCAAT55750.0103.057362
GTCTCAA17150.097.746651
CCCTCGC554.129106E-1092.3611451
GGCTCAA62900.091.95211
GGGTAGT25550.090.037281
TAGCATT235600.090.0335164
CTAGCAT270850.078.755023
GGTAGTA33400.068.85122
AGAGCCA12000.067.661115
AGGGTAG9650.065.366673
CACATTA30750.063.184677145
TAGAGCC13600.060.767464
ATAGAGC13250.060.192343
AGTAGAG37850.059.948845
ACCGGCC3850.056.491436145
Produced by FastQC (version 0.11.5)