FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-40_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-40_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4252583
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCT59811614.06476957651385No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGT48849111.48692453504141No Hit
CTCCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCAT3345577.867148036851955No Hit
TTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACA1478633.4770162040341126No Hit
CCCTAGCATTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTT767941.805820133316622No Hit
GGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAAT671791.579722253510396No Hit
TTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTCACCA664241.561968337831384No Hit
ATATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTC500691.1773785485198056No Hit
GAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCT489661.1514413710443747No Hit
CTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCTGT441191.037463583897128No Hit
AGCATTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACA418830.9848837753431268No Hit
GTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATG328830.7732476944012616No Hit
CTCCCTAGCATTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGG319900.7522486921478075No Hit
GGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGG308230.724806546985679No Hit
GTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTCACCAAT282170.6635261439929567No Hit
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCCTGTCTCTTATAC273900.6440791396664097No Hit
ATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCAC273540.6432325953426423No Hit
GGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGCTGTCTCTTATAC263680.6200466869194557No Hit
GCATTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAG246600.5798828617807107No Hit
CTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACA227120.5340754078168493No Hit
GCTCTCCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCG198030.4656699234324174No Hit
GGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGAGCTGTCTCTTAT194200.45666363243233576No Hit
GCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTCA188330.4428602569309053No Hit
TCTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCTGTCTCTTATAC187930.4419196521267192No Hit
CTCCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCCTGTCTCTTAT182080.42816330686549803No Hit
TCTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCAATG179730.4226372536409048No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGACTGTCTCTTATAC174130.4094687863822999No Hit
CAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGG172650.40598854860681144No Hit
ATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTCACC150260.3533381946924963No Hit
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTCTCTTATACA147910.3478121414679031No Hit
GTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGCTGTCTCTTATACA144540.33988754599263554No Hit
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCACTG125900.2960553621175648No Hit
GTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGAGCTGTCTCTTATA118290.27816035571792486No Hit
TGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGCTG118280.2781368405978202No Hit
CTCCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTCTCTTATA116390.27369248289804105No Hit
AGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTC112620.26482728261858735No Hit
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCC102350.24067725427111006No Hit
GTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCTGT102180.24027749722933095No Hit
GATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGC96440.22677981828926091No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACCTGT87400.20552214971465577No Hit
GTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGG85610.20131294321592313No Hit
CCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCC84810.19943173360755095No Hit
CCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTGAATATTGGTGA83550.1964688284743649No Hit
TGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGAGC77620.18252436225230642No Hit
CTCCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCAC76830.18066666776403892No Hit
CCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTAGCATTGTGAAC72630.17079031732008523No Hit
CTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCAATGC69870.16430014417120137No Hit
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCCTGT68340.16070233079518967No Hit
GCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGCTGT66660.15675179061760816No Hit
TCTCCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCA64180.15092004083165456No Hit
GATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGA62470.1468989552937591No Hit
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGCTGTCTCTTAT61350.14426526184203814No Hit
CAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGCTGTCTCTTAT60570.1424310824738753No Hit
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGCACCTGTTTGCGCATCT59110.13899787493859614No Hit
GAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTCACCAATAT58380.1372812711709566No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCCAATGCTAGGGCTGTC57350.13485921380017746No Hit
CTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCTGTCTCTTATACA56950.13391860899599137No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGC54760.12876879769307267No Hit
CTCCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCCT54570.1283220104110843No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGCTGCGCAAACAGGT54280.12764007192804938No Hit
CCCTAGCATTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACCTGTC53560.12594698328051446No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGCTGTCTCTTATACA52700.12392468295151442No Hit
CTCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATC52460.12336032006900277No Hit
CCCTAGCATTGTGCACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCT52130.12258432110554926No Hit
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCCTGTCT51950.12216104894366554No Hit
GCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGCTGTCT50590.11896299260943291No Hit
CTAGCATTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA49850.11722287372168867No Hit
GCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGAGCT48510.11407184762766535No Hit
GCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAA47590.1119084565780374No Hit
CCCAATGCTAGGGAGAGCTGCCTAGCATTGGGTGCCTGGAATATTGGTGA43710.10278458997743255No Hit
GGTTCACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAAT43370.10198507589387437No Hit
GAATATGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTT42740.10050362332728133No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTC100.0070898863145.012187
CTCCCTA628250.0119.308081
TCCCTAG633150.0118.599852
CGTTATG203.8558818E-4108.8282552
TACCTAG4050.0107.48472
CCCTAGA10150.0103.698441
CTACCTA4250.0102.4784551
CTCCATA4850.097.2840961
CGAGAAC301.5986465E-596.683888
GGAGCGA900.096.661146
GAATTGT10600.093.697496
GGATATT7450.092.5630341
GATATTG7300.092.4294742
AGAATTG12650.090.54185
CGGAGAT1050.089.755724
CTAATGT4400.087.436641
CCCTAGC1596300.087.331321
ATATGGT8900.086.3535463
CCCTAGG1100.085.786891
TCGGAGA1100.085.6870043
Produced by FastQC (version 0.11.5)