FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-40_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-40_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5707993
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCAAATATTCAAATGAGAACTTTGAAGACCGAAGTGGAGAAGGGTTC316730.554888557151349No Hit
GTGCAGGTATATGCAAGAGCAAAGAAAAGGAGGCTCTGAAGCATATAGGA231540.40564170278414846No Hit
GGTATATGCAAGAGCAAAGAAAAGGAGGCTCTGAAGCATATAGGAGGAGT203750.35695558841785546No Hit
CCATCAAGCTTGAGGAATATCAACCTCATTCCCTTTTGACAATGTATTCG156760.2746324321000394No Hit
GTAGAAATATTCAAATGAGAACTTTGAAGACCGAAGTGGAGAAGGGTTCC133340.23360224863625445No Hit
GTGTTAAATAACTAGGAATTTTTTAAAAAACAAACAAACAAAACAAAACA109820.1923968722456387No Hit
GTATTCGTAAGAATACATGTATTACAAGGAATTTCCCTATGTCTTAGGAT108040.1892784381480496No Hit
GGTTATGGTGATGGTGGTGGAGGTGGTAGTAGAGGTGGTGATGGTGGTTA99810.1748600602698707No Hit
CCCAAAGAGAGATTACTAAGAGTTTCATTTAAAATCTTTTTTTTTTTTTT90080.15781378848922906No Hit
CCATAACCACCATCACCACCTCTACTACCACCTCCACCACCATCACCATA88140.1544150457087106No Hit
CCCTTTTGACAATGTATTCGTAAGAATACATGTATTACAAGGAATTTCCC83390.14609338168424524No Hit
GTTTTAGGCATTATCCAAATCCAAACTGATTTGCAACACTTTTATGAGAG82380.14432393312325367No Hit
ATTTTGTGCTGACATCCGGGTAAGACAGAACCACAGGAAACCCACGGGAA81110.14209898295250187No Hit
ATATCAACCTCATTCCCTTTTGACAATGTATTCGTAAGAATACATGTATT76970.1348459957817047No Hit
GATACATGCAGTGTGGTCTCTGGACCTCTAAGCCTCTGCTTCATCAGGAA76670.13432041700121217No Hit
CCATCAGCTTGAGGAATATCAACCTCATTCCCTTTTGACAATGTATTCGT76580.13416274336706438No Hit
GTTCCTAGGTAGTAGAAATGAGATCAGGCACCTGATTAAAGTAGCAGTCT76280.13363716458657185No Hit
CCATAACCATCACCACCTCTACTACCACCTCCACCACCATCACCATAACC74260.1300982674645887No Hit
GGTGATGGTGGTTATGGTGATGGTGGTGGAGGTGGTAGTAGAGGTGGTGA73310.12843393465969563No Hit
GTAGAAATACTAGGTAAAAAACAGCAAACCAAACCAAACAGTAAGGAACA72820.12757548931822446No Hit
ATTCAAATGAGAACTTTGAAGACCGAAGTGGAGAAGGGTTCCATGAGAAC70610.12370372563526269No Hit
CATCAAGCTTGAGGAATATCAACCTCATTCCCTTTTGACAATGTATTCGT68970.12083056163523676No Hit
GTTCCTAGGTAGTAGAAATACTAGGTAAAAAACAGCAAACCAAACCAAAC68190.11946405680595615No Hit
GAATACATGTATTACAAGGAATTTCCCTATGTCTTAGGATCGAGTAACCC66730.11690624007422573No Hit
GGGTAAGACAGAACCACAGGAAACCCACGGGAACAGGTCCAAGTGGATGA66020.11566237029372671No Hit
GTTCCTAGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACCGAAGTG65240.11429586546444609No Hit
GTTCCTAGGTAGTAGAAATAGAGGTTAAGGAGGGTGATGGTGGCTATGAT65070.11399803748883364No Hit
GATATTCCCTTGCTTTACAACAATGGGTTGGCCACCCAAAGAGAGATTAC65040.1139454796107844No Hit
ACAATGGGTTGGCCACCCAAAGAGAGATTACTAAGAGTTTCATTTAAAAT64970.11382284456200278No Hit
ATCCTAAGACATAGGGAAATTCCTTGTAATACATGTATTCTTACGAATAC64500.1129994378058978No Hit
GTATATGCAAGAGCAAAGAAAAGGAGGCTCTGAAGCATATAGGAGGAGTT61130.10709543617169817No Hit
GGTAAGGGCTCCTGGGGGTCAAACGCATTTCAGCAAATCTTCCTGATGAA59730.10464273519606629No Hit
CCATAACCACCATCACCACCTCTACTACCACCTCCACCACCACCACCATA58200.1019622834155543No Hit
TTATAAGGACTGTGGCATGGACTGGCTTTCCTTAACAGCACTGGAAGCTT58110.10180460978140653No Hit
GCATGTATCAGCCACAAACCTCTCATGGGGCCAAAAGCTACCTGTTCTGA57890.10141918534237865No Hit
ATTTAACACTGAGCTATTCCTTTGTATGTAAACCTTCAATTAAAAGAAAA57350.10047314353749208No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA96450.0113.6434561
TCCTAGG119700.091.833813
CCTAGGT126900.086.326334
GCAGGTA57500.084.994263
AGGTATA58100.084.846015
CAGGTAT66400.074.465794
TAGGTAG148700.074.160076
GTAGCAA69800.072.172841
TTCCTAG167000.065.595642
CTAGGTA171450.063.931215
GTGCAGG83500.058.9403841
TGCAGGT83600.058.8352972
CCGCTTA11800.056.5351569
GGTATAT88100.055.6317446
TAGCAAC41250.054.903541
ATCAAGC47200.053.30713
TAGCAAA95350.052.650192
AGGTAGT211400.052.0649347
TTTGTGC32700.051.222623
TGCTGAC32800.050.8431557
Produced by FastQC (version 0.11.5)