FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-39_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-39_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12206303
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT565880.4635965533544432No Hit
GTAGCAAAAGCTGCATATGATGGAAGGGAACTAAACTCTGAGGCTATAGC405300.33204156901561427No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG370540.3035644781224913No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC326880.26779607224234886No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC315110.25815351298423445No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA283790.2324946382209257No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT281200.23037278363481553No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA250220.20499245348898845No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG245800.2013713734617271No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC232080.19013127889746798No Hit
GTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGA225120.1844293067278438No Hit
GTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCT219610.17991524542689133No Hit
GGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTA218530.17903045664194966No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT216750.17757219364454577No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT214360.17561418883342483No Hit
CCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCT209810.17188660645242054No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA205620.16845395366639676No Hit
TTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGAATCACCATTAGC197210.1615640706281009No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC196620.16108071379188277No Hit
CCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGT195270.15997472781070565No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT195190.15990918790071No Hit
CAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGA193750.15872946952078776No Hit
CACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATC193540.15855742725704908No Hit
GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG192580.15777094833710092No Hit
CTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCC192500.15770540842710523No Hit
CTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACT191820.15714831919214195No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT191050.15651749755843355No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA188050.15405975093359556No Hit
CCCTAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAG187870.15391228613610525No Hit
ACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGC183230.15011097135635582No Hit
GCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTAC181790.14893125297643356No Hit
GCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTA179130.14675205096907723No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG179000.14664554861533421No Hit
GTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAAC174870.14326205076180726No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC170100.13935423362831484No Hit
GTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTT168800.13828921009088502No Hit
GTATAGATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAA168680.1381909002258915No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA163030.1335621440824466No Hit
AGTAAATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTA162980.1335211816386993No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC162060.13276747267374897No Hit
ATGTGGTACCATGTCACCGTCTATTCTAAACTTAAAGAAGTCATGTTTAG160640.13160413927132564No Hit
GTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTTGATAATGTTG156890.12853195599027814No Hit
GTACCAGTGTGTGTACACAACATCTTAACACAATTAGTGATTGGTTGTCC155980.12778643951407728No Hit
ATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTG149800.12272348146691099No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG148650.12178134526072308No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG147110.12051970199330625No Hit
GTCCATACTAATTTCACTAAGTTGAACAATTTTACTATCTGCATCTACAA146230.11979876298335378No Hit
GTATTATCTTTCTGTGCTTTTGCTGTAGATGCTGCTAAAGCTTACAAAGA146100.11969226062961079No Hit
ATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGAAAAGCAT145650.11932359863588508No Hit
AAATAGAGGTATGGTACTTGGTAGTTTAGCTGCCACAGTACGTCTACAAG144280.11820122767720906No Hit
GTTGTAAGAGGTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTT144000.11797183799222419No Hit
ATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTT141020.1155304763448851No Hit
CATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGTTGCATGG139890.11460472511619611No Hit
GTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGGTA139760.11449822276245314No Hit
GCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGT136000.11141784699265618No Hit
CTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTATACATTTG135860.11130315215016372No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG134440.1101398187477404No Hit
GACTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGC133940.1097301943102674No Hit
CTCTTAGGGAATCTAGCCCATTTCAAATCCTGTAAATCGGATAACAGTGC133120.10905841023281168No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT132330.10841120362160435No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT131200.10748545239291536No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG130940.1072724476854294No Hit
GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT130440.10686282324795641No Hit
TGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGA129580.10615826921550285No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC127380.10435592169062163No Hit
CATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGTTA126600.10371690756816375No Hit
ACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGC126390.10354486530442511No Hit
CTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTACAAGGTGGTTCCAGT125550.10285669624947047No Hit
CATGAAGACAGTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCA125410.102742001406978No Hit
GACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTC125230.10259453660948774No Hit
TCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCTATTGAAACAC124880.10230779950325664No Hit
TAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCT122560.1004071421133819No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCAAT36400.0107.834342
TCAATGT37050.0106.834484
GAATATT18200.0102.904831
TCCCTAG29100.0101.97452
CCCTAGC93050.099.2334751
CCTAGCA95050.097.629192
AGGGTAG8850.095.041333
GTCTCAA18150.094.789271
ACGAGGT2800.088.1012652
CTCCCTA35350.084.1941451
GGGTAGT33500.083.4263841
GCTCGGA1350.080.657541
AAGGGTA10650.079.025932
GACGAGG3050.078.541931
GCCGACC4650.071.71825145
GAAGGGT12500.067.365171
GGTGAAC28950.065.120968
GAGCTGA15750.064.065131
GAAGACA36200.062.481734
CGTTACG350.003568874762.1366425
Produced by FastQC (version 0.11.5)