FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-38_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-38_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10653276
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGCACAGCTACCCTCTAGGAGCTTCTGGACACATGGAGAAGACAAA780090.7322536278981226No Hit
ACACTGGAAAGTTCACCTTCAGCTTCCAGGCACAGCTGTGTGGCTCCAAA583590.5478033235973611No Hit
ATGTTAAATGGACTCACCTTGATTATGAGTTCCAGGTCTGTCAAGACACA558600.5243457505465924No Hit
CAATGTGTCCAGTGTGAATTCAACTTTATCAACACTGGAAAGTTCACCTT504330.4734036741374203No Hit
GTCCAGTGTGAATTCAACTTTATCAACACTGGAAAGTTCACCTTCAGCTT410790.3855996972198974No Hit
CTCTAGGAGCTTCTGGACACATGGAGAAGACAAAGAACACACAGGAGAAT377980.3548016591328339No Hit
GTCCAACACAGCTACCCTCTAGGAGCTTCTGGACACATGGAGAAGACAAA354450.3327145565364119No Hit
TTAAAGAAGTGTGTCTTGACAGACCTGGAACTCATAATCAAGGTGAGTCC302840.2842693646536521No Hit
GTCTCAAGGGCTGTTGTTTTATCAGAGCCTAGCCTACCTGAGAGAAAAGT297570.2793225295204968No Hit
ATGTAGGACAGAGTGTACATGCCACCCTGTCTTTTCAACCATTAAAGAAG296950.27874054891659616No Hit
AATGTGTCCAGTGTGAATTCAACTTTATCAACACTGGAAAGTTCACCTTC296070.27791451193041466No Hit
CTTCTGGACACATGGAGAAGACAAAGAACACACAGGAGAATTAACCTGCC287940.2702830565921694No Hit
CAATGTGTCCAGCGTGTACAGACCCTCCATGTCCGCTGCCTTGTGCGGGT279320.26219164884116397No Hit
CCCTAGCATTCACCAATGACCTGAACGGTGAGCACCCGCACAAGGCAGCG271600.25494505164420783No Hit
CCCTAGCATTGACGGGATTCTGTGGGTGAAGTCAATCAGAGTATGCTCAG254260.2386683683028582No Hit
GTACAGACCCTCCATGTCCGCTGCCTTGTGCGGGTGCTCACCGTTCAGGT251860.23641554015872676No Hit
ATTTAACATTGTCCAAACTCCAAAAGTACTCTCAGCATGCTGCCTTGGCA249320.23403129703952097No Hit
GTGCAGGAATCGCCGGCCTGTATCTCATAAGCATAAACATTCACAAACTC242950.2280519156736388No Hit
GAATTAACCTGCCAAGGCAGCATGCTGAGAGTACTTTTGGAGTTTGGACA242590.2277139914520191No Hit
CTCAAGGGCTGTTGTTTTATCAGAGCCTAGCCTACCTGAGAGAAAAGTAA234630.2202421114406498No Hit
GTCTATGGAGGGATAATGAGAACTGCTGGGGGAGCAGCAGCATACTACAG232390.21813947183946047No Hit
GTCCAGTAAGGTCAGGAGGCATAGAACTCATGGTTAAAGTTTTGGAGATT230870.21671268068151053No Hit
GTCAAGACACACTTCTTTAATGGTTGAAAAGACAGGGTGGCATGTACACT230790.2166375864100395No Hit
CCTGTTGTCTATGGAGGGATAATGAGAACTGCTGGGGGAGCAGCAGCATA228370.2143659846980403No Hit
GTGTGAATTCAACTTTATCAACACTGGAAAGTTCACCTTCAGCTTCCAGG223090.20940976278095114No Hit
GTGTACATGCCACCCTGTCTTTTCAACCATTAAAGAAGTGTGTCTTGACA221600.20801113197480287No Hit
GTTTTATAGTGAGCCTATGCACTGTGAACTTCACCAGTGTTGTGCAGGCT219530.2060680677004895No Hit
GTCCTACATCCACAGTGCTGTCGATGGGTGAAAATTCCAAGTACTGCAGC215460.20224764663939995No Hit
GAGTATGCTCAGTGCAGGAATCGCCGGCCTGTATCTCATAAGCATAAACA211650.1986712819605913No Hit
GTATGTATGTGACTGGCAATATGTCAAATGCCTGTTGAGAGTTTGGAGAG211110.1981643956281617No Hit
CATGGAGAAGACAAAGAACACACAGGAGAATTAACCTGCCAAGGCAGCAT208720.19592095426796416No Hit
GCATAGAACTCATGGTTAAAGTTTTGGAGATTTCTTTTTTTGCAAGTTTT208410.19562996396601384No Hit
CTTTTGGAGTTTGGACAATGTTAAATGGACTCACCTTGATTATGAGTTCC203720.19122756230102364No Hit
GTTTAGGCAGGATTCTGCCCTTGGAAACTGCTGGAATTTAACTAAAGATA199310.18708799058618214No Hit
CAATGTGTCCAGCACAGCTACCCTCTAGGAGCTTCTGGACACATGGAGAA199010.1868063870681657No Hit
GAGTGTACATGCCACCCTGTCTTTTCAACCATTAAAGAAGTGTGTCTTGA195180.18321124882148926No Hit
GTATTTATGAAGAGAAGTCACCTTAAAGGCAGAGACTGCAGCATTTCTTT193750.1818689387189443No Hit
CTTCTTTAATGGTTGAAAAGACAGGGTGGCATGTACACTCTGTCCTACAT192000.1802262515305151No Hit
GATTATGAGTTCCAGGTCTGTCAAGACACACTTCTTTAATGGTTGAAAAG191240.17951285595154018No Hit
GTTTTATCAGAGCCTAGCCTACCTGAGAGAAAAGTAATACCTAATCCCAG190640.1789496489155073No Hit
GGAGAATTAACCTGCCAAGGCAGCATGCTGAGAGTACTTTTGGAGTTTGG184610.17328941820337707No Hit
GGCTCAATGTGTCCAGTGTGAATTCAACTTTATCAACACTGGAAAGTTCA183870.17259479619226986No Hit
AATGTGTCCAGCGTGTACAGACCCTCCATGTCCGCTGCCTTGTGCGGGTG177200.16633381130837124No Hit
CCCTCTAGGAGCTTCTGGACACATGGAGAAGACAAAGAACACACAGGAGA175620.16485069944681804No Hit
GTGTCTTGACAGACCTGGAACTCATAATCAAGGTGAGTCCATTTAACATT173570.16292640874037245No Hit
AGCGTGTACAGACCCTCCATGTCCGCTGCCTTGTGCGGGTGCTCACCGTT172850.16225056029713303No Hit
GTTAAATGGACTCACCTTGATTATGAGTTCCAGGTCTGTCAAGACACACT170370.15992263788153052No Hit
ATCAAGGTGAGTCCATTTAACATTGTCCAAACTCCAAAAGTACTCTCAGC169540.1591435348150184No Hit
AGCACTGACGGGATTCTGTGGGTGAAGTCAATCAGAGTATGCTCAGTGCA168990.15862726169865493No Hit
CCTTAAAGGCAGAGACTGCAGCATTTCTTTTTGTCCTACCATTATTCTAG168870.1585146202914484No Hit
CACCTTGATTATGAGTTCCAGGTCTGTCAAGACACACTTCTTTAATGGTT167140.15689070667088695No Hit
TATCAACACTGGAAAGTTCACCTTCAGCTTCCAGGCACAGCTGTGTGGCT167050.15680622561548202No Hit
GTACAACATGCATGTTGTATGTATGTGACTGGCAATATGTCAAATGCCTG165790.15562349083981303No Hit
GTTGTCTATGGAGGGATAATGAGAACTGCTGGGGGAGCAGCAGCATACTA165060.1549382556126397No Hit
GGCTCAATGTGTCCAGCGTGTACAGACCCTCCATGTCCGCTGCCTTGTGC163980.15392448294778055No Hit
CTACAGTTCTGTGATTAGGTTTCAGTTTTATAGTGAGCCTATGCACTGTG160780.15092071208893865No Hit
GCATACTACAGTTCTGTGATTAGGTTTCAGTTTTATAGTGAGCCTATGCA154480.14500703821059363No Hit
CCCTAGCATTCATCGCCATATCTTGCTTCTCTTTTTCCTTAGAGCTCCTT154430.1449601042909242No Hit
TCTTTAATGGTTGAAAAGACAGGGTGGCATGTACACTCTGTCCTACATCC154320.14485684966765153No Hit
ACTTTATCAACACTGGAAAGTTCACCTTCAGCTTCCAGGCACAGCTGTGT154310.14484746288371764No Hit
GTGGATGTAGGACAGAGTGTACATGCCACCCTGTCTTTTCAACCATTAAA153900.14446260474242853No Hit
GTTCATTGTATCCCTAACCTTAACCAGATCACCACAGAACTCTCCAAACT152430.14308274750414804No Hit
CCCTAGCATTGTAGGCAGCTCTCCTAGGCAGCTCTCCCTAGCATTGGTGC150520.14128987177277674No Hit
GGAGAAGACAAAGAACACACAGGAGAATTAACCTGCCAAGGCAGCATGCT150340.1411209096619669No Hit
CTGGAAAGTTCACCTTCAGCTTCCAGGCACAGCTGTGTGGCTCCAAAACC149690.1405107687062646No Hit
CCTTGATTATGAGTTCCAGGTCTGTCAAGACACACTTCTTTAATGGTTGA147090.13807020488345556No Hit
CACCTGTTGTCTATGGAGGGATAATGAGAACTGCTGGGGGAGCAGCAGCA146770.13776982779757138No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAATGCTAGGGAGAGCT146230.1372629414651418No Hit
CATACATACAACATGCATGTTGTACACTGCAACTCTTAAAAGAGCTGGGA145670.13673728156484447No Hit
CAGGAGAATTAACCTGCCAAGGCAGCATGCTGAGAGTACTTTTGGAGTTT142140.1334237468361845No Hit
CCCTAGCATTGTAGGCAGCTCTCCTAGGCAGCTCTCCCTAGCATTGTGAA139170.13063587200782184No Hit
GTACATGCCACCCTGTCTTTTCAACCATTAAAGAAGTGTGTCTTGACAGA139040.13051384381668138No Hit
GTACTTGGAATTTTCACCCATCGACAGCACTGTGGATGTAGGACAGAGTG137250.12883360949251665No Hit
GAATTCAACTTTATCAACACTGGAAAGTTCACCTTCAGCTTCCAGGCACA136410.12804511964207066No Hit
TAATCAAGGTGAGTCCATTTAACATTGTCCAAACTCCAAAAGTACTCTCA135860.1275288465257072No Hit
GTGTACAACATGCATGTTGTATGTATGTGACTGGCAATATGTCAAATGCC135400.12709705446474867No Hit
GCTACCCTCTAGGAGCTTCTGGACACATGGAGAAGACAAAGAACACACAG134310.12607389501595564No Hit
CGACAGCACTGTGGATGTAGGACAGAGTGTACATGCCACCCTGTCTTTTC134220.12598941396055072No Hit
GGTTCACAATGCTAGGGAGAGCTGCCTAGGAGAGCTGCCTACAATGCTAG132150.12404634968623736No Hit
GCTGTGTGGCTCCAAAACCTTGCTGCAGTACTTGGAATTTTCACCCATCG131580.12351130300200615No Hit
GTAATACCTAATCCCAGCTTCCTCTAGCCATCCTATCTTGCCTAAGGCAG130860.12283545455876671No Hit
CCACAGAACTCTCCAAACTCTCAACAGGCATTTGACATATTGCCAGTCAC129170.12124908807394082No Hit
TGTACACTCTGTCCTACATCCACAGTGCTGTCGATGGGTGAAAATTCCAA127830.11999125902680077No Hit
ACCTTAACCAGATCACCACAGAACTCTCCAAACTCTCAACAGGCATTTGA127600.11977536299632151No Hit
CAGTAAGGTCAGGAGGCATAGAACTCATGGTTAAAGTTTTGGAGATTTCT127280.11947498591043731No Hit
GGCTCAATGTGTCCAGCACAGCTACCCTCTAGGAGCTTCTGGACACATGG126980.11919338239242089No Hit
CCATTATTCTAGTTATTTCCTGATTCCCAGCTCTTTTAAGAGTTGCAGTG124840.11718461063057034No Hit
GTCCAGCGTGTACAGACCCTCCATGTCCGCTGCCTTGTGCGGGTGCTCAC124160.11654630932306645No Hit
CAGCTACCCTCTAGGAGCTTCTGGACACATGGAGAAGACAAAGAACACAC123440.115870460879827No Hit
CCTATGCACTGTGAACTTCACCAGTGTTGTGCAGGCTCCTCCCTGCCTTA122860.11532602741166191No Hit
GTAGGACAGAGTGTACATGCCACCCTGTCTTTTCAACCATTAAAGAAGTG122580.11506319746151325No Hit
GTATCTCATAAGCATAAACATTCACAAACTCACACAGTTTAGGCAGGATT121600.1141432926359929No Hit
ACTTGGAATTTTCACCCATCGACAGCACTGTGGATGTAGGACAGAGTGTA121210.11377720806257155No Hit
CAGCTGTGTGGCTCCAAAACCTTGCTGCAGTACTTGGAATTTTCACCCAT120730.11332664243374527No Hit
TCCAGGCACAGCTGTGTGGCTCCAAAACCTTGCTGCAGTACTTGGAATTT120630.11323277459440646No Hit
GGTTAAGGTTAGGGATACAATGAACAGAGTATCTTTAGTTAAATTCCAGC116130.10900872182416002No Hit
CCCTTGTATTTATGAAGAGAAGTCACCTTAAAGGCAGAGACTGCAGCATT115390.10831409981305282No Hit
GTTGTACACTGCAACTCTTAAAAGAGCTGGGAATCAGGAAATAACTAGAA114930.10788230775209429No Hit
GTGCATAGGCTCACTATAAAACTGAAACCTAATCACAGAACTGTAGTATG114900.10785414740029264No Hit
GTTCACCTTCAGCTTCCAGGCACAGCTGTGTGGCTCCAAAACCTTGCTGC113510.10654938443348319No Hit
CTCCAAAACCTTGCTGCAGTACTTGGAATTTTCACCCATCGACAGCACTG112150.10527278181847537No Hit
ACCTAATCCCAGCTTCCTCTAGCCATCCTATCTTGCCTAAGGCAGGGAGG112010.10514136684340103No Hit
CTATGGAGGGATAATGAGAACTGCTGGGGGAGCAGCAGCATACTACAGTT111510.10467202764670698No Hit
GGAAAGTTCACCTTCAGCTTCCAGGCACAGCTGTGTGGCTCCAAAACCTT111090.10427778272148397No Hit
AAGATACTCTGTTCATTGTATCCCTAACCTTAACCAGATCACCACAGAAC110740.10394924528379815No Hit
GCACTGTGGATGTAGGACAGAGTGTACATGCCACCCTGTCTTTTCAACCA110110.10335787789596364No Hit
CTTTTAAGAGTTGCAGTGTACAACATGCATGTTGTATGTATGTGACTGGC109640.10291669905107124No Hit
GAACACACAGGAGAATTAACCTGCCAAGGCAGCATGCTGAGAGTACTTTT109000.10231594487930286No Hit
GTTCACCAATATTCCAGGCACCAATGCTAGGGAGAGCTGCCTAGGAGAGC108300.10165887000393117No Hit
GCATTGACGGGATTCTGTGGGTGAAGTCAATCAGAGTATGCTCAGTGCAG108140.1015086814609891No Hit
GACCCAATACAAAGTCATGATCCCATCCTCAATGCCTAGACCTCAGCCAA108070.10144297397345192No Hit
GGGATAATGAGAACTGCTGGGGGAGCAGCAGCATACTACAGTTCTGTGAT107910.10129278543050983No Hit
AATGTGTCCAGCACAGCTACCCTCTAGGAGCTTCTGGACACATGGAGAAG106810.10026023919778292No Hit
CCTCTAGCCATCCTATCTTGCCTAAGGCAGGGAGGAGCCTGCACAACACT106680.10013821100664246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCAAC59250.098.504941
GGCTCAA163350.097.1007461
CAACACA74550.077.116024
TCCAACA75950.075.752132
CTGACGG35650.075.2386255
TGAAGAC32250.074.198833
CATGAAG32450.074.046341
GGGTAGT44100.073.41531
CCAACAC83750.072.296043
TCTCAAG135850.071.0299452
ATTGACG63750.069.839488
AGCACAG198250.068.379685
AGCATTC116850.066.3825155
GTCCACT41300.066.2645951
TTCATCG36350.064.8353969
CAGCACA208450.064.6193854
GAAGGGT23400.063.2855571
TGTTGTC63300.062.202663
CAGCTAC308150.061.514389
CTCAAGG150350.060.720473
Produced by FastQC (version 0.11.5)