FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-38_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-38_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8281505
Sequences flagged as poor quality0
Sequence length151
%GC46

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGAAAAGAGGGCAAAAAATGGAACTCTTGATACATAACTACTGTGG815970.9852919245958313No Hit
CTATAAGTGGGTGAATAGAGATATCATTCCTTGGAATTTTCCACACAAAA653950.7896511564021275No Hit
ATAGAAAAGAGGGCAAAAAATGGAACTCTTGATACATAACTACTGTGGGC363760.43924383309555454No Hit
GCACTAACCTCTGAGGGCAGCATACCTGGAATCCCTAGAGTCCCTGCCAG314890.38023282000071246No Hit
TACTATAAGTGGGTGAATAGAGATATCATTCCTTGGAATTTTCCACACAA304570.3677713169285051No Hit
GCAACAGCCTGCACATGGAATAGAAAAGAGGGCAAAAAATGGAACTCTTG264770.3197124194213491No Hit
CCTCTGAGGGCAGCATACCTGGAATCCCTAGAGTCCCTGCCAGTCCAGGA258060.3116100274044392No Hit
TAACTACTGTGGGCCAGAATTGATATATATGACTTCTACTTACAATCCAC249160.300863188514648No Hit
ATATATGACTTCTACTTACAATCCACTGGCCAGAGCTAATCAATGGTCAA245620.2965886031584839No Hit
TAGCAACAGCCTGCACATGGAATAGAAAAGAGGGCAAAAAATGGAACTCT245270.2961659746628179No Hit
AGCCTGCACATGGAATAGAAAAGAGGGCAAAAAATGGAACTCTTGATACA245080.2959365477651707No Hit
GGTGATGGTGGTTATGGTGATGGTGGTGGAGGTGGTAGTAGAGGTGGTGA242010.29222949210318655No Hit
GAATTGATATATATGACTTCTACTTACAATCCACTGGCCAGAGCTAATCA234170.2827626138002694No Hit
GGTTATGGTGATGGTGGTGGAGGTGGTAGTAGAGGTGGTGATGGTGGTTA233260.28166377971153794No Hit
TGGTAGTAGAGGTGGTGATGGTGGTTATGGTGATGGTGGTGGAGGTGGTA219160.2646378888861385No Hit
CATAACTACTGTGGGCCAGAATTGATATATATGACTTCTACTTACAATCC214480.25898674214409095No Hit
GGTGAATAGAGATATCATTCCTTGGAATTTTCCACACAAAAAAGAACAAT212220.2562577695720766No Hit
GTCCAGGATACTATAAGTGGGTGAATAGAGATATCATTCCTTGGAATTTT197730.23876094985150645No Hit
GGATACTATAAGTGGGTGAATAGAGATATCATTCCTTGGAATTTTCCACA196760.237589665163518No Hit
ATCACGCACTAACCTCTGAGGGCAGCATACCTGGAATCCCTAGAGTCCCT192380.23230077141775557No Hit
CCATAACCACCATCACCACCTCTACTACCACCTCCACCACCATCACCATA191630.23139513892704286No Hit
GTAGTAGAGGTGGTGATGGTGGTTATGGTGATGGTGGTGGAGGTGGTAGT189630.2289801189518089No Hit
GGTGATGGTGGTGGAGGTGGTAGTAGAGGTGGTGATGGTGGTTATGGTGA181190.2187887346563215No Hit
ACTATAAGTGGGTGAATAGAGATATCATTCCTTGGAATTTTCCACACAAA179490.21673596767737266No Hit
GATATATATGACTTCTACTTACAATCCACTGGCCAGAGCTAATCAATGGT168690.2036948598111092No Hit
ATAAGTGGGTGAATAGAGATATCATTCCTTGGAATTTTCCACACAAAAAA160560.19387780361178314No Hit
TAGTAGAGGTGGTGATGGTGGTTATGGTGATGGTGGTGGAGGTGGTAGTA156400.18885456206329646No Hit
ACCACCACCATAACCACCATCACCACCTCTACTACCACCTCCACCACCAT150220.1813921503398235No Hit
CCCTAGAGTCCCTGCCAGTCCAGGATACTATAAGTGGGTGAATAGAGATA145350.1755115767001288No Hit
ATACATAACTACTGTGGGCCAGAATTGATATATATGACTTCTACTTACAA143920.17378483741783649No Hit
GATCACGCACTAACCTCTGAGGGCAGCATACCTGGAATCCCTAGAGTCCC135250.16331572582519724No Hit
CTTGATACATAACTACTGTGGGCCAGAATTGATATATATGACTTCTACTT135030.16305007362792148No Hit
TCCCTAGAGTCCCTGCCAGTCCAGGATACTATAAGTGGGTGAATAGAGAT130630.15773702968240674No Hit
TGGTTATGGTGATGGTGGTGGAGGTGGTAGTAGAGGTGGTGATGGTGGTT127150.15353489492549965No Hit
ATATCATTCCTTGGAATTTTCCACACAAAAAAGAACAATGACTGCTGATA126380.1526051122350346No Hit
GGTTATGGTGGTGGTGGTGGAGGTGGTAGTAGAGGTGGTGATGGTGGTTA126230.15242398573689203No Hit
TCTGAGGGCAGCATACCTGGAATCCCTAGAGTCCCTGCCAGTCCAGGATA121940.14724376789001517No Hit
TGGTAGTTATGGTGATGGTGGTGGAGGTGGTAGTAGAGGTGGTGATGGTG120420.14540835270883734No Hit
CCATAACCACCATCACCACCTCTACTACCACCTCCACCACCAACACCATA110380.13328495243316282No Hit
GTAGAGGTGGTGATGGTGGTTATGGTGATGGTGGTGGAGGTGGTAGTAGA109560.1322947942433169No Hit
GGCCAGAGCTAATCAATGGTCAACTTGCTGGAAAAGACAGCAAATTACAG106980.12917941847526507No Hit
ATTAGCAACAGCCTGCACATGGAATAGAAAAGAGGGCAAAAAATGGAACT106530.12863603898083742No Hit
CCTCTACTACCACCTCCACCACCATCACCATAACCACCATCACCACCTCT106460.12855151328170425No Hit
GCACATGGAATAGAAAAGAGGGCAAAAAATGGAACTCTTGATACATAACT105170.1269938253976783No Hit
CTACTACCACCTCCACCACCATCACCATAACCACCATCACCACCTCTACT103550.1250376592177388No Hit
ATATATATGACTTCTACTTACAATCCACTGGCCAGAGCTAATCAATGGTC101360.1223932123448576No Hit
GTCCCTGCCAGTCCAGGATACTATAAGTGGGTGAATAGAGATATCATTCC92480.11167052365481879No Hit
CGCACTAACCTCTGAGGGCAGCATACCTGGAATCCCTAGAGTCCCTGCCA86510.10446168902874538No Hit
GGTGATGGTGGTGGAGGTGGTAGTAGAGGTGGTGATGGTGGTTATGGTGG84640.10220364535190163No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA85350.0119.9031
TTCCTAG106750.095.479542
TCCTAGG109450.093.28133
CCTAGGT109300.093.062454
CTAGGTA119800.085.261785
TAGCAAC85850.062.9840281
ATTAGCA35300.062.7104071
TAGGTAG164850.062.581746
GGTTACG49850.062.023881
ACGCACT61850.051.81074
ATCACGC63600.050.212291
TCACGCA64350.049.4900442
GTTACGG65000.047.4326552
GCACTAA109800.047.3948671
TTACGGT65800.046.945233
CGCACTA69150.046.7565965
TACGGTG69300.044.6716044
TTAGCAA50550.043.1963652
AATAGAA308050.042.836442
ACAGCCT140400.042.291564
Produced by FastQC (version 0.11.5)