FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n02_CV-37_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n02_CV-37_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12741675
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT265970.20874021665126447No Hit
GTAGCAAAAGCTGCATATGATGGAAGGGAACTAAACTCTGAGGCTATAGC259820.20391353570076148No Hit
GTACTTTTCTGTGTCTTTGATTTCGAGCAACATAAGCCCGTTAATACAAA232290.18230727121826606No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC226870.17805351337245692No Hit
GAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCAT224290.1760286618517581No Hit
GTATAGGCAGAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAG212750.1669717678405704No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT210730.16538641897552714No Hit
GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT203540.15974351880737814No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT201910.15846425214895216No Hit
CTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATA190470.1494858407548458No Hit
ATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAAGTAGT185240.14538119988149126No Hit
CTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTTTG183550.14405484365281646No Hit
ACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATC179360.14076642199710793No Hit
CCCTAGCATTGTTCACTGTACACTCGATCGTACTCCGCGTGGCCTCGGTG172280.1352098527077484No Hit
GTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAA168850.13251789894185811No Hit
CTTTCTGTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCA168610.1323295406608629No Hit
CTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCC161240.12654537178196745No Hit
ACATAAGCCCGTTAATACAAACTGGTGTACCAACCAATGGAGCTTCAACA161040.12638840654780475No Hit
GTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTCCTCTGTTAAC157120.12331188795821585No Hit
GTATTCACTGAGACTCATTGATGCTATGATGTTCACATCTGATTTGGCTA153800.12070626507111507No Hit
GTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAAA152550.1197252323575982No Hit
TCCTTAAACTTCTCTTCAAGCCAATCAAGGACGGGTTTGAGTTTTTCATA149960.11769253257519126No Hit
CATGAAGACAGTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCA148710.1167114998616744No Hit
CTTCAATAGTCTGAACAACTGGTGTAAGTTCCATCTCTAATTGAGGTTGA148290.11638187286993273No Hit
GATTAAAGATTGCTATGTGAGATTAAAGTTAACTACATCTACTTGTGCTA147830.11602085283135852No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT147720.11593452195256904No Hit
CATATGTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGA142020.11146101277893213No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG140430.11021313916733867No Hit
CTTCAGTACCATACTCATATTGAGTTGATGGCTCAAACTCTTCTTCTTCA139510.10949109909019025No Hit
TTCATAAACAGTGCCAAAGATGTTAGTTAGCCACTGCGAAGTCAACTGAA137270.10773308846756804No Hit
CTTTATGCATTTGCATCAGAGGCTGCTCGTGTTGTACGATCAATTTTCTC136180.10687762794138132No Hit
GGCTTATGTTGCTCGAAATCAAAGACACAGAAAAGTACTGTGCCCTTGCA135740.1065323044262234No Hit
GTGATAGAAGTGCAAGGTTACAAGAGTGTGAATATCACTTTTGAACTTGA130880.10271804923606981No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA130730.10260032531044781No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC129850.10190967828013194No Hit
GGTTACAAGAGTGTGAATATCACTTTTGAACTTGATGAAAGGATTGATAA129040.101273969081773No Hit
GTATTGTTCTTTCTACCCTCCAGATGAGGATGAAGAAGAAGGTGATTGTG128810.10109345906248589No Hit
CTAGTAGGTTGTTCTAATGGTTGTAAATCACCAGTTTTCAAGACAACTTC128600.10092864556661507No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC128230.10063825988341409No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGC42600.097.472661
TCCCTAG14100.095.708672
GAATATT46050.094.74171
CCTAGCA45350.091.831592
TCAATGT21100.084.179274
GCCGACC5100.083.873314145
GCTCAAT21350.082.9181062
GGAATAT10300.062.021431
GCTGCCC7300.059.58959145
AGTGGCC8350.056.43765145
CCCTCGC651.0860756E-555.8409731
GCTCGGA651.0860756E-555.8409731
TCGGACC400.006050223554.3801963
GCGCATC5750.054.2140585
AATATTG83600.053.1132132
CATGAAG49100.051.746731
CGCCTAA5050.045.9996951
TTGCGCA6800.045.8499683
TGACGCG5550.044.414818145
GAACGTT19000.044.2658237
Produced by FastQC (version 0.11.5)