FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97633122
Sequences flagged as poor quality0
Sequence length151
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19741072.0219644312920773No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC3712140.38021318216168487No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG2501130.25617638243709956No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC2465770.25255466070213345No Hit
GTAGCAAAAGCTGCATATGATGGAAGGGAACTAAACTCTGAGGCTATAGC2455610.2515140302488739No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT2331890.2388421011467809No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC2096030.21468431584109335No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT2053500.21032821218192735No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC2038180.20875907256146126No Hit
CCCTAGCATTGGTGCCTGGAATATTGGTGAACGCTACTAATGTTGTTATT1812380.18563167528331215No Hit
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCT1686630.17275182493908164No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT1581770.16201161732798014No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC1575380.16135712632440452No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA1566130.16040970194520668No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGT1542540.15799351371760909No Hit
CCCTAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAG1304170.13357864352632295No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT1213560.12429798158047226No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG1129620.1157004894302161No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC1081800.11080256145040615No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG1043350.10686434876065931No Hit
TTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGAATCACCATTAGC1033800.10588619710429827No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA1019900.10446249992907121No Hit
CAATAACAGATGCGCAAACAGGTTCAGTAGTAGGCAGCTCTCCCTAGCAT1019260.10439694840445643No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA1016840.10414908170200683No Hit
CTCCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCAT978450.10021701446769264No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGCG194650.0151.2509145
GTTCCTA764900.0100.822511
TTCCTAG877900.087.547872
TAGGTAG955050.081.663166
ATTTTTT1598650.067.76999145
CCTAGGT1218250.064.150354
CTAGGTA1253300.062.479995
AGGTAGT1260450.062.059057
TCCTAGG1600100.049.08673
AGAGCCG77650.047.487957145
TAAGCGT101950.045.64196145
GTAGATC972800.043.1040654
GGGGAAC182000.039.39572145
GACGGGC218400.037.0075047
CCGTGCG101350.035.3064589
CCCTAGC7290450.033.0517231
CGCTAGT118800.032.1753439
CCTAGCA7592650.031.8544082
TAGCATT7611250.031.3362274
CATCCAG464300.031.0766189
Produced by FastQC (version 0.11.5)