FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-53_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-53_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13124126
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCTAGGTAGTAGAAATAGGAAAAGGAGTCCTCTTCCTATTTCTACCC939760.7160553015111254No Hit
GTCCCAGAGTGGTTGCTATGAGGTTGCAGCAGCAGTCTGATGACAGAAGG508110.3871572095543734No Hit
GTGGAAGATCGTTGGTGTTATGTCCCAGAGTGGTTGCTATGAGGTTGCAG408270.3110835723460747No Hit
CTCGTATAGTTTCAACTTATTCCACTCCACTGGCTTTTCCTCAACAACGG361510.27545453312472007No Hit
GACTTATTCCCTGGGCTACTCTCGTATAGTTTCAACTTATTCCACTCCAC318940.24301808745207112No Hit
TCCTATTTCTACCCTTCCTGTCTCCTTCATGACTAAGACTTATTCCCTGG311660.23747105140563265No Hit
TTTCTACCCTTCCTGTCTCCTTCATGACTAAGACTTATTCCCTGGGCTAC277680.2115798034855807No Hit
GGTAGGGAGAGATGTAGGATCAAAGTATTTGTTTCTAAAACATTGAAGAG275330.20978920805850235No Hit
ATTTCTACCCTTCCTGTCTCCTTCATGACTAAGACTTATTCCCTGGGCTA274700.20930917609294514No Hit
ATCAAATGCCACCTGAAGGTCAAACATGACAAGAAAGAGTCCATTGGAAT265620.2023906201449148No Hit
ACATTGAAGAGACTTAAATTTTATCCGTTGTTGAGGAAAAGCCAGTGGAG265470.20227632681978214No Hit
GACTAAGACTTATTCCCTGGGCTACTCTCGTATAGTTTCAACTTATTCCA262230.19980759099691667No Hit
CCCAAAGACAGTGCAAAAGATGGAATACAGAAATTTTTTTTCTCTGGTCT238390.18164257185583252No Hit
GATGTAGGATCAAAGTATTTGTTTCTAAAACATTGAAGAGACTTAAATTT226940.17291818137070614No Hit
GGTATATGCAGGGCATCCCAGAGCCCAAAGTGTTCTCCAGACCGTCTGCT225770.17202669343467136No Hit
GTGGAGATAGTGGGCAGGCAGTTTGGCTGTGGAGTGAAAAAGAGAGAAAG223650.17041134777279646No Hit
GAAAGGGAGTGAGTGGGTAGGGAGAGATGTAGGATCAAAGTATTTGTTTC217560.16577103877241045No Hit
AAGTATTTGTTTCTAAAACATTGAAGAGACTTAAATTTTATCCGTTGTTG207250.15791527755829227No Hit
CTCTCGTATAGTTTCAACTTATTCCACTCCACTGGCTTTTCCTCAACAAC206920.1576638322430004No Hit
CTCCCTACCCACTCACTCCCTTTCTCTCTTTTTCACTCCACAGCCAAACT203130.15477602089464854No Hit
CTTCAATGTTTTAGAAACAAATACTTTGATCCTACATCTCTCCCTACCCA201600.15361022897829538No Hit
GGAGAGATGTAGGATCAAAGTATTTGTTTCTAAAACATTGAAGAGACTTA196120.14943471283344886No Hit
GTTTCAACTTATTCCACTCCACTGGCTTTTCCTCAACAACGGATAAAATT193570.14749172630619364No Hit
GTAGTAGAAATAGGAAAAGGAGTCCTCTTCCTATTTCTACCCTTCCTGTC192070.14634879305486706No Hit
AGGAAAAGGAGTCCTCTTCCTATTTCTACCCTTCCTGTCTCCTTCATGAC188870.14391053545203697No Hit
ATGTAGGATCAAAGTATTTGTTTCTAAAACATTGAAGAGACTTAAATTTT187790.14308762351108179No Hit
CCACTGGCTTTTCCTCAACAACGGATAAAATTTAAGTCTCTTCAATGTTT186810.1423409071202151No Hit
CCATCAAATGCCACCTGAAGGTCAAACATGACAAGAAAGAGTCCATTGGA179360.1366643386386263No Hit
ACTTATTCCACTCCACTGGCTTTTCCTCAACAACGGATAAAATTTAAGTC177550.13528519918202553No Hit
GACTTAAATTTTATCCGTTGTTGAGGAAAAGCCAGTGGAGTGGAATAAGT176520.13450038501611458No Hit
AGGTAGTAGAAATAGGAAAAGGAGTCCTCTTCCTATTTCTACCCTTCCTG175100.13341840820485876No Hit
GTGTTATGTCCCAGAGTGGTTGCTATGAGGTTGCAGCAGCAGTCTGATGA173800.13242786605370901No Hit
ACAAGAAAGAGTCCATTGGAATTGCCCAGTGGAAGATCGTTGGTGTTATG172460.13140684568252395No Hit
GAAAAAGAGAGAAAGGGAGTGAGTGGGTAGGGAGAGATGTAGGATCAAAG164820.1255855056557671No Hit
TTCCTCAACAACGGATAAAATTTAAGTCTCTTCAATGTTTTAGAAACAAA160400.12221766234185803No Hit
ATTTAAGTCTCTTCAATGTTTTAGAAACAAATACTTTGATCCTACATCTC159400.12145570684097365No Hit
CCATAACCACCATCACCACCTCTACTACCACCTCCACCACCATCACCATA151340.11531434550384537No Hit
GTATTTGTTTCTAAAACATTGAAGAGACTTAAATTTTATCCGTTGTTGAG143280.10917298416671709No Hit
CTATGAGGTTGCAGCAGCAGTCTGATGACAGAAGGCCAAGGAATGAGTGA142960.10892915840643408No Hit
CTATCTCCACTCCCCGCCAGCTCACTCATTCCTTGGCCTTCTGTCATCAG139640.10639946614349786No Hit
GAATTGCCCAGTGGAAGATCGTTGGTGTTATGTCCCAGAGTGGTTGCTAT138070.10520319600710935No Hit
GAGATGTAGGATCAAAGTATTTGTTTCTAAAACATTGAAGAGACTTAAAT137540.10479935959164062No Hit
GTCTGATGACAGAAGGCCAAGGAATGAGTGAGCTGGCGGGGAGTGGAGAT136790.10422789296597731No Hit
GGGTAGGGAGAGATGTAGGATCAAAGTATTTGTTTCTAAAACATTGAAGA134870.10276493840427926No Hit
CCACCATCAAATGCCACCTGAAGGTCAAACATGACAAGAAAGAGTCCATT134810.10271922107422621No Hit
GAATGAGTGAGCTGGCGGGGAGTGGAGATAGTGGGCAGGCAGTTTGGCTG134110.10218585222360713No Hit
TTCCTAGGTAGTAGAAATAGGAAAAGGAGTCCTCTTCCTATTTCTACCCT133910.10203346112343024No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA367550.0127.6572341
TCCTAGG408800.0115.225073
TTCCTAG411800.0113.930272
CCTAGGT456700.0103.124064
TAGGTAG481750.097.972436
CTAGGTA482000.097.846445
AGGTAGT611750.077.126447
TGTAGAG137950.073.335882
TTAGCAA101300.068.319822
TTGTAGA150500.065.444341
GTAGAGC174000.058.9737173
GTATATG139550.058.1622122
TATATGC144900.057.2639923
TAGAGCA181600.057.263854
ATTAGCA131000.053.056241
ATATGCA103850.052.2660684
AGGTATA67800.048.7393385
CGTGGAC26700.045.0548253
CTCGTGG66300.044.7094151
GGTATAT196500.044.2227551
Produced by FastQC (version 0.11.5)