FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-52_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-52_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12355129
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG473170.38297455251175444No Hit
ATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTT398380.3224409878682772No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA374630.3032182019305505No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC368300.29809482361535844No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT364490.2950110840607168No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT355120.2874271891454958No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG321040.2598435030504336No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG306600.2481560492002957No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC302120.244530024737095No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC298080.2412601276765301No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG290780.2353516503146183No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC287480.23268069479484998No Hit
CCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGT283220.22923273403296718No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC271550.21978726405851365No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC261910.21198483641894794No Hit
GTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTT256730.2077922456333722No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG247520.2003378515918369No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA240730.19484215826479837No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG239100.19352286811412492No Hit
GTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTAGA239070.19349858670030884No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT237830.19249495492924434No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA235410.1905362542147476No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA232290.18801098717787568No Hit
CAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTT209340.16943570560857762No Hit
GTCTCAAGGGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGT207560.16799500838882378No Hit
AATTAAATGTTGGTGATTATTTTGTGCTGACATCACATACAGTAATGCCA189140.15308622030575317No Hit
CATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGTTA188180.15230921506363876No Hit
GGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTA187230.15154030362612966No Hit
GTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACATCA185660.15026957630308838No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG184320.14918500648597033No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT183850.14880459766951848No Hit
GACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTG182050.14734771284055392No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT181410.14682970934581097No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG177670.14380262642340683No Hit
CTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCC176980.1432441539056371No Hit
CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTT173440.14037894707534013No Hit
TCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCTATTGAAACAC172200.13937531530427566No Hit
CTCAAGGGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTG169410.1371171438193806No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT167130.13527175636935884No Hit
AGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACAT164720.1333211494594674No Hit
CAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGA162990.13192092126274035No Hit
GTAGCAAAAGCTGCATATGATGGAAGGGAACTAAACTCTGAGGCTATAGC161130.13041547360614364No Hit
ATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAAGTAGT157940.12783354993703425No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT157700.12763929862650564No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC157100.12715367035018413No Hit
GTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCT156810.12691895001662873No Hit
TTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGAATCACCATTAGC156540.12670041729228404No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT156330.1265304473955715No Hit
GTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTA155200.125615847475166No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT154540.1250816563712123No Hit
GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT154310.12489549886528907No Hit
CATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGT153710.12440987058896753No Hit
CTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACT152060.12307439282908338No Hit
GTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGA151670.12275873444947437No Hit
TCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAA148620.12029012404484No Hit
CCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAAC148380.1200958727343114No Hit
CCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCT147010.11898702150337727No Hit
CACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATAC146930.11892227106653439No Hit
ACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACT146650.11869564453758435No Hit
GCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTAC144250.11675313143229829No Hit
GTATAGGCAGAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAG142960.115709030638207No Hit
GACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCT142710.11550668552307305No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA142350.11521530855728014No Hit
GTCCAACATTTTGCTTCAGACATAAAAACATTGTTTTGATAATAAAGAAC142350.11521530855728014No Hit
GAAGGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACC141540.11455971038424609No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT140070.11336992110725837No Hit
GCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTA138010.11170259735855449No Hit
GTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCA137020.11090131070262399No Hit
GAGTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACTCATTCA136840.11075562221972754No Hit
GTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTT135690.10982483469011128No Hit
GCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGT135340.10954155152892375No Hit
GTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTCTCAGT135280.1094929887012916No Hit
CATAAAAACATTGTTTTGATAATAAAGAACTGACTTAAAGTTCTTTATGC134570.10891832857431114No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC134460.10882929672365219No Hit
GTAGATAAAAGTGCATTAACATTGGCCGTGACAGCTTGACAAATGTTAAA134120.10855410736706997No Hit
CACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATC132690.1073966933085037No Hit
CATGAAGACAGTGTTTAGCAAGATTGTGTCCGCTTAAAACACAACTACCA131280.10625546685914813No Hit
CATATGTATTGTTCTTTTTACCCTCCAGATGAGGATGAAGAAGAAGGTGA131150.10615024739927846No Hit
GAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTCCCTGG130180.10536514835255868No Hit
GTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTC128760.10421582809859777No Hit
GTACTTTTCTGTGTCTTTGATTTCGAGCAACATAAGCCCGTTAATACAAA128650.1041267962479388No Hit
CTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTTTG128360.10389207591438342No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG127770.10341454144266725No Hit
CTCCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAG126940.10274275566042247No Hit
GTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAAC126630.10249184771765638No Hit
ATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTAT126450.10234615923475991No Hit
GTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACTCATTCACA126320.10224093977489025No Hit
GCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGT125730.1017634053031741No Hit
CTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCC124560.10081643016434713No Hit
ACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCGTCAGAGA124010.10037127091105241No Hit
CACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCAC123570.10001514350841663No Hit
CTTTCTGTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCA123560.10000704970381127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGTA19450.0109.530922
AGGGTAG19500.0109.248753
GTCTCAA31650.0107.155961
GCTCAAT31150.0106.075672
TCAATGT31700.0104.691154
TCCCTAG27400.0102.3456652
GAAGGGT21500.098.758231
CCCTAGC79100.096.654061
GGGTAGT76400.096.465451
CCTAGCA81550.093.74272
CTCCCTA35550.079.296521
GGTAGTA99750.073.805682
GAATATT22050.069.016811
GAAGACA33500.068.351184
AGTAGAG113350.065.3333365
AGTGGCC9400.059.37405145
GTAGTAG126450.058.220863
CAATGTG59300.056.698135
GCCCACC14200.055.638058145
TCCAAGG18550.055.46942
Produced by FastQC (version 0.11.5)