FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-51_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-51_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10690815
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATAACAGATGCGCAAACAGGTTCAGTAGTAGGCAGCTCTCCCTAGCAT2378302.2246199190613622No Hit
CCCTAGCATTGGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGCA2367512.2145271431598057No Hit
CCCTAGCATTGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGCAA1980441.8524686845670792No Hit
ATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTGTTATTG1314871.2299062325931185No Hit
GCATTGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGCAAACAGG1169781.0941916027917422No Hit
GCATTGGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGCAAACAG1142161.068356341401474No Hit
CATGAAGACAGTGTGGTATAGGCACAAAGATAGAAAATAAATCAGTGAAA1079621.0098575272324888No Hit
CATTGAGCCCACAATAACAGATGCGCAAACAGGTTCAGTAGTAGGCAGCT918030.8587090881284543No Hit
CACAATAACAGATGCGCAAACAGGTTCAGTAGTAGGCAGCTCTCCCTAGC911530.8526291026455888No Hit
GCCTACTACTGAACCTGTTTGCGCATCTGTTATTGTGGGCTCAATGTGTC857400.8019968543090493No Hit
CTCCCTAGCATTGGGTGCCTGGACACATTGAGCCCACAATAACAGATGCG796830.7453407434325634No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCAGTAGTAGGCAGCTCTCC632170.5913206804158523No Hit
GACACATTGAGCCCACAATAACAGATGCGCAAACAGGTTCAGTAGTAGGC612980.5733706925056696No Hit
AGCATTGGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGCAAACA612570.572987185729058No Hit
CCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTACTACTGAACCT553990.5181924857927109No Hit
CCCTAGCATTGAGCCCACAATAACAGATGCGCAAACAGGTTCAGTAGTAG553930.518136362849792No Hit
GTGTGGGACTGGGTTCCCGGGTTCGTTTCTAAAGAAAGATAACCTACTGT536180.5015333255696596No Hit
CTCCCTAGCATTGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGC501560.4691503875055363No Hit
CATGAAGACAGTGTGGCAATTCCTCAAGCTTCTCCTTGGTGAAGGAATGA439350.4109602495226042No Hit
GCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTGTTATTGTG422800.3954796711008468No Hit
CTACTGAACCTGTTTGCGCATCTGTTATTGTGGGCTCAATGTGTCCAGGC406320.38006456944582806No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAATGCTAGGGAGAGCT363740.34023598762114954No Hit
GTGCCTGGACACATTGAGCCCACAATAACAGATGCGCAAACAGGTTCAGT363460.33997408055419537No Hit
GTGTGGTATAGGCACAAAGATAGAAAATAAATCAGTGAAATAAAACAGAG338000.31615924510900245No Hit
GGACACATTGAGCCCACAATAACAGATGCGCAAACAGGTTCAGTAGTAGG328110.3069083133512272No Hit
CCAATATTCCAGGCACCAATGCTAGGGAGAGCTGCCTACTACTGAACCTG318910.29830279543701765No Hit
CCCAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTGTT290480.2717098743173462No Hit
GGTTCACAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATC278710.2607004236814499No Hit
GTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTACTACTG275650.25783815359259327No Hit
TGCCTGGACACATTGAGCCCACAATAACAGATGCGCAAACAGGTTCAGTA229950.21509117873613937No Hit
GTAGTAGGCAGCTCTCCCTAGCATTGGGTGCCTGGAATATTGGTGAACAG217150.2031182842468044No Hit
TTGGTGAACAGATGCGCAAACAGGTTCAGTAGTAGGCAGCTCTCCCTAGC208460.19498981134740428No Hit
CCCTAGCATTGAGCCCACAATCTGGACACATTGAGCCCACAATAACAGAT206530.19318452335018424No Hit
CCTGGACACATTGAGCCCACAATAACAGATGCGCAAACAGGTTCAGTAGT203500.19035031473278696No Hit
ATTGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGCAAACAGGTT197420.18466318985035285No Hit
GCCCACAATAACAGATGCGCAAACAGGTTCAGTAGTAGGCAGCTCTCCCT196720.18400842218296734No Hit
ATGCTAGGGAGAGCTGCCTACAATGCTAGGGAGAGCTGCCTACTACTGAA195770.183119808920087No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGC195200.1825866409623588No Hit
ATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTGTTCACC193780.1812583979799482No Hit
CCCTAGCATTGGTGCCTGGAATATTGGTGGGTGCCTGGAATATTGGTGAA182780.17096919177817593No Hit
GCATTGAGCCCACAATAACAGATGCGCAAACAGGTTCAGTAGTAGGCAGC181970.1702115320487727No Hit
TTGGTAGACTTTAATAACAACATTAGTAGCGGAACAGATGCGCAAACAGG181530.16979996380070184No Hit
CACCAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTGT177740.1662548645730003No Hit
ATTGAGCCCACAATCTGGACACATTGAGCCCACAATAACAGATGCGCAAA175240.16391640861805204No Hit
AGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTGTTATTGTGGGC173680.16245721210216432No Hit
CTTCTGTGGTGGCACGTGGGTCATCAGTTGAGTTGTCCACACATGGGTGG166090.15535765982294147No Hit
CCCTAGCATTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTT162090.15161613029502427No Hit
GTATAGGCACAAAGATAGAAAATAAATCAGTGAAATAAAACAGAGAACAG159720.14939927404973335No Hit
GGTATAGGCACAAAGATAGAAAATAAATCAGTGAAATAAAACAGAGAACA157850.14765010899543204No Hit
TGTGTGGACAACTCAACTGATGACCCACGTGCCACCACAGAAGAGTGGGG155090.1450684536211692No Hit
GTGTAGGGCTGAGCTGCAGGCTGGGTCTTCAGGTGGTGTCCCTGCACATC154100.1441424250630097No Hit
AAATAAATCAGTGAAATAAAACAGAGAACAGCAAAACAGATCCACCCATG148340.13875462254280896No Hit
CACCTGGAATGTGTTTTTGTGAATGGTGTGGGACTGGGTTCCCGGGTTCG145990.1365564739451576No Hit
CATTAGTAGCGCAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGC144710.13535918449622408No Hit
GTCCAGACTTTAATAACAACATTAGTAGCGCAATGCTAGGGAGAGCTGCC144380.13505050831017093No Hit
GCCATTGCCTGAGATAGGTAAAACAGAAGTAAACGCCCTGGCAGCCCCCA143900.13460152476682088No Hit
GTTCACCAATATTCCAGGCACCAATGCTAGGGAGAGCTGCCTACTACTGA142130.1329458979507175No Hit
GCGCATCTGTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCC142020.1328430058886998No Hit
GTGGTATAGGCACAAAGATAGAAAATAAATCAGTGAAATAAAACAGAGAA137410.1285308931077752No Hit
CCCTAGCATTGGCTGACCTCTAGCATACTCTGTCAAACTACAGGCAGCTG137250.12838123192665854No Hit
GGCACCAACAGATGCGCAAACAGGTTCAGTAGTAGGCAGCTCTCCCTAGC136260.12745520336849903No Hit
GCCTACTACTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACC135220.12648240569124056No Hit
CTATTATCTGCATTATAGTTAAAAACATCGACATGAACAAACAAAAGCCC134210.12553766948544148No Hit
ATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTT133240.12463034857492156No Hit
CTCCCTAGCATTGAGCCCACAATAACAGATGCGCAAACAGGTTCAGTAGT131880.1233582285354297No Hit
AGCATTGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGCAAACAG131600.1230963214684755No Hit
GCCATTCACCTGGAATGTGTTTTTGTGAATGGTGTGGGACTGGGTTCCCG129710.12132844876653463No Hit
GATAACCTACTGTCCCGTTCTTGTCTAAGGAGGAGACAGCCCTTGCCCCA129640.12126297199979608No Hit
TTGGTGAACAGATGCGCAAACAGGTTCACAATGCTAGGGAGAGCTGCCTA128120.11984119077918755No Hit
GCCTACAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCT127840.11957928371223335No Hit
GTCCCGTTCTTGTCTAAGGAGGAGACAGCCCTTGCCCCACTCTTCTGTGG127510.11927060752618017No Hit
AAATAAAACAGAGAACAGCAAAACAGATCCACCCATGTGTGGACAACTCA127400.11916771546416245No Hit
CATTGGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGCAAACAGG125030.11695085921887152No Hit
CTAGCATTGGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGCAAA124900.11682925950921423No Hit
TTGGTAGACTTTAATAACAACATTAGTAGCGCAATGCTAGGGAGAGCTGC124460.11641769126114333No Hit
GGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGCAAACAGGTTCA121380.11353671352464709No Hit
CACCCAATGCTAGGGAGAGCTGCCTACTACTGAACCTGTTTGCGCATCTG120720.11291936115254075No Hit
CATCTGTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTAC120410.11262939261412717No Hit
TAACAGATGCGCAAACAGGTTCAGTAGTAGGCAGCTCTCCCTAGCATTGG120030.11227394730897503No Hit
TCTGTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTACTA117180.10960810752033404No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCAATGCTAGGGAGAGCT116510.10898140132440792No Hit
GCCTACTACTGAACCTGTTTGCGCATCTGTTATTGTGGGCTCAATGCTAG115730.10825180306646408No Hit
CTGTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTACTAC114150.10677389890293677No Hit
GTAGTAGGCAGCTCTCCCTAGCATTGGTGCCTGGAATATTGGTGGGTGCC113200.10588528564005645No Hit
GGTGAACAGATGCGCAAACAGGTTCAGTAGTAGGCAGCTCTCCCTAGCAT111830.10460381177674481No Hit
GTAGTAGGCAGCTCTCCCTAGCATTGGGTGCCTGGACACATTGAGCCCAC110220.10309784614175813No Hit
TTGGTGCCTGGACACATTGAGCCCACAATAACAGATGCGCAAACAGGTTC109350.10228406346943614No Hit
GTAGTAGGCAGCTCTCCCTAGCATTGCGCTACTAATGTTGTTATTAAAGT109070.10202215640248194No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCAGACACATTGAGCCCACA108030.10104935872522348No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGAAG210100.0121.802791
ATGAAGA224100.0114.512622
GACAGTG232550.0110.8118747
AGACAGT252250.0102.245876
TGAAGAC291650.089.5023
GAAGACA307950.083.564044
GACGAGG14300.080.076781
CAGTGTG328900.076.9568259
AAGACAG373200.074.604455
ACAGTGT400800.067.237718
ACGAGGT17100.066.962292
CGAGGTC18300.062.9646873
GGTAGCA16650.052.6691971
GAAATTG43200.050.9965553
TCATAGC104400.049.9824521
GTAGCAG17200.048.8763582
GTAGACT110700.048.7705464
TCTGCCA77850.048.4987033
TGAAATT45800.047.9453432
GGTAGAC112100.047.9031033
Produced by FastQC (version 0.11.5)