FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-51_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-51_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15054164
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC4068962.7028800802223225No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC3562002.3661227551393753No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG3074892.042551150631812No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC2613741.7362239444183019No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT1490160.9898656610888522No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC1393010.9253320210939645No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG1366460.9076957046568643No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC1167030.7752207296267No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG1104550.7337172625460969No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT1076280.7149384050818098No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC1049580.6972024484388505No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA863100.5733297445145409No Hit
ACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACG828690.5504722812904125No Hit
CTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGG793970.5274088949741745No Hit
AACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCA780000.5181290704684763No Hit
ATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCC736160.4890075596359918No Hit
TTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCG694490.461327510448272No Hit
CCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGA665970.44238258597421953No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC644510.4281273938559458No Hit
AACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCA638740.42429456727055714No Hit
CCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGA633440.42077394666352774No Hit
GTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCC612140.406625037431504No Hit
CTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTC610700.4056684914552545No Hit
GTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGGAACGC596580.3962890267436969No Hit
GTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAG584310.3881384579044044No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG572600.38035987916698666No Hit
TTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACC564410.3749195239270676No Hit
CTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATT547150.36345425757285493No Hit
CTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCGA545240.3621855056182462No Hit
ATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGA536980.3566986516155929No Hit
GATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAA516620.34317415434028753No Hit
GGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACC510950.3394077545588051No Hit
CTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCT484080.32155887234920516No Hit
CACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTC461000.3062275660076508No Hit
CTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATT454220.30172382870280945No Hit
GGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGG448460.29789764479781145No Hit
AAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAAT445180.2957188456296876No Hit
GGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGC427010.2836490953599283No Hit
ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTC425440.28260619453860075No Hit
ACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCAT424470.2819618545407105No Hit
GCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTC422270.28050046485477376No Hit
CCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAG420040.2790191471276651No Hit
CATCTGGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAAT417030.27701970033008805No Hit
CTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAA413670.2747877597188393No Hit
CAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCA409100.2717520547803252No Hit
GGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC408860.27159263045095033No Hit
CCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCG408270.27120071230790366No Hit
GTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTG401640.2667966152089216No Hit
TCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGG397220.26386055047626694No Hit
ACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATA396860.26362141398220457No Hit
CACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCA396380.2633025653234547No Hit
CCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGG387620.25748357730127025No Hit
CCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTA373800.24830339300143137No Hit
TGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTG372850.24767233836432231No Hit
GAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTT371340.24666929362533846No Hit
ATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTC365300.2426571146694031No Hit
AAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCAC352650.2342541239752669No Hit
CCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTG348950.23179633223073698No Hit
CCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATG348730.23165019326214326No Hit
GATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAA344620.22892005162159787No Hit
CAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAA341880.22709995719456758No Hit
AATTAACACCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAG339890.22577806379683388No Hit
CCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTC335320.2227423588583199No Hit
CTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGT333880.2217858128820704No Hit
AATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGC323950.21518963125418322No Hit
CCAATAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGA322420.21417330115441813No Hit
ATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCA321180.21334960878598108No Hit
GGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAG318490.21156272776090387No Hit
GATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGG308260.2047672657212981No Hit
ATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTT303400.20153892305145607No Hit
GCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATT300620.19969225790286327No Hit
CTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGAT293840.19518852059802191No Hit
GCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAG292080.19401940884927255No Hit
GTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCC291000.19330199936708542No Hit
GAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGT288810.1918472523615393No Hit
AAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAA275670.18311877032826268No Hit
GAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAA272260.18085361631506072No Hit
GAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACG263760.17520733798303248No Hit
TCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAAT263170.1748154198399858No Hit
GTTTTGATCGCGCCCCACTGCGTTCTCCATTCTGGTTACTGCCAGTTGAA263040.17472906499490773No Hit
CAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAAT262800.1745696406655328No Hit
ACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGG261420.17365295077162704No Hit
GAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTGGGGC248240.16489789801678792No Hit
GCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAAC243100.16148356029600847No Hit
GAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTC241550.16045394483546213No Hit
CTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGC241220.16023473638257163No Hit
CTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTC234400.15570442835616777No Hit
GACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGCT233840.15533243825429296No Hit
GCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGG231970.15409025702124676No Hit
CTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGACCAA227640.15121397641210763No Hit
GGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCA227280.15097483991804525No Hit
AATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTT223870.14870968590484335No Hit
GTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTGG222340.14769335580507825No Hit
CGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGC220140.14623196611914152No Hit
GCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCA219700.14593968818195419No Hit
GTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAATTCGT219520.145820119934923No Hit
TCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCG218150.14491007272140785No Hit
ATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTA214840.14271134551211215No Hit
ACCGAAGAGCTACCAGACGAATTCGTGGTGGTGACGGTAAAATGAAAGAT212500.1411569583007067No Hit
TAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACG212400.14109053149680048No Hit
ATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAAT211550.14052590366359766No Hit
TCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTTAAATTCCC211550.14052590366359766No Hit
CCAAGACGCAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATC208400.13843345934055187No Hit
ACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACACCAATAGCAGT206530.13719127810750567No Hit
ACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTA201910.13412235976703854No Hit
GGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCG200520.13319902719274215No Hit
TGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAATTAACA200340.13307945894571097No Hit
CCAAAACGCAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATC200280.13303960286336725No Hit
GAATTCGTCTGGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTG199370.13243511894782067No Hit
AGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGT197650.13129257792063379No Hit
ATTCTGGTTACTGCCAGTTGAATCTGAGGGTCCACCAAACGTAATGCGGG196520.13054195503649357No Hit
ACGTAATGCGGGGTGCATTTCGCTGATTTTGGGGTCCATTATCAGACATT196250.1303626026659468No Hit
CCACTGCGTTCTCCATTCTGGTTACTGCCAGTTGAATCTGAGGGTCCACC195850.1300968954503219No Hit
CTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTG195070.12957876637985344No Hit
GTCTGGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTG191910.12747967937641705No Hit
GATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGG187370.12446390247907489No Hit
GCGTTCTCCATTCTGGTTACTGCCAGTTGAATCTGAGGGTCCACCAAACG186860.12412512577915319No Hit
TTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCG182460.12120234640727974No Hit
CAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAA182010.12090342578970177No Hit
GGACAAGGCGTTCCAATTAACACCAATAGCAGTCCAGATGACCAAATTGG180990.12022587238985838No Hit
CTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGT180900.12016608826634279No Hit
TCTGGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGG177870.11815335610798447No Hit
TTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAA176540.11726987961603183No Hit
ATCTACAACAGTAGCAACAATGGAAGTAACTTCAGGCTCAGGAACAACTG176510.11724995157485994No Hit
CATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGC176120.11699088703962571No Hit
ATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTT173980.11556935343603272No Hit
GGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGT173850.11548299859095462No Hit
ATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAAC173450.11521729137532978No Hit
ACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCT171060.11362969076197124No Hit
ACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCCAATTT169660.11269971550728423No Hit
TCTGGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTT169630.11267978746611236No Hit
ATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGT168100.1116634573663473No Hit
ACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTGGTTACTGCCAG167520.11127818190369122No Hit
ACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAA167210.11107225881158198No Hit
CCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTC167140.11102576004884761No Hit
GTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATAATACT164770.10945144479627032No Hit
TAGCAGTCCAGATGACCAAATTGGCTACTACCGAAGAGCTACCAGACGAA164440.10923223634337982No Hit
GAACCAAGACGCAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTG161420.10722614686541213No Hit
ACGCAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCC161210.10708665057720906No Hit
GGTTACTGCCAGTTGAATCTGAGGGTCCACCAAACGTAATGCGGGGTGCA161130.10703350913408409No Hit
CCATTCTGGTTACTGCCAGTTGAATCTGAGGGTCCACCAAACGTAATGCG160740.10677444459884985No Hit
TTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGA160440.10657516418713121No Hit
AGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGA156860.10419708460728873No Hit
GATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGG156330.10384502254658579No Hit
TCTCCATTCTGGTTACTGCCAGTTGAATCTGAGGGTCCACCAAACGTAAT155720.10343981904275786No Hit
AGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGGAACGCCTTGT155370.10320732522908613No Hit
ACCATCAACAGCTCCAGTCCCTACAACAGAAATTCCGACCACAATGAGCA154550.10266262543705516No Hit
CTCACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGT152710.10144037224518081No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA583400.0140.804721
TCCTAGG695700.0118.63793
TTCCTAG697200.0117.732562
CCTAGGT719750.0114.552884
CTAGGTA742400.0111.224645
TAGGTAG764000.0108.231836
AGGTAGT938050.088.2409447
CTTGTAG847750.068.038211
TGTAGAG56550.063.0480352
GTAGATC941750.061.5260284
GTAGAGC59250.059.5610473
TGTAGAT978350.059.490993
ATCTGTT1014300.057.5653238
TTGTAGA1009400.057.426382
TAGATCT1015050.057.1119775
GGACCGA12450.056.4630781
TCTGTTC1058500.055.154719
AGATCTG1075100.054.4409756
TTAGCAA49100.051.2138022
GGTATAT56750.050.825231
Produced by FastQC (version 0.11.5)