FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-50_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-50_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9938531
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGCATTGGTGCCTGGAATATTGGTGAACGCTACTAATGTTGTTATT2734862.7517748850408577No Hit
CCCTAGCATTGTAGGCAGCTCTCTCTCTCGCTACTAATGTTGTTATTAAA1305321.3133932972589208No Hit
CCCTAGCATTGCGCTACTAATGTTGTTATTAAAGTCTAGGCAGCTCTCCC1040931.0473680667696261No Hit
CTACTAATGTTGTTATTAAAGTCTAGGCAGCTCTCCCTAGCATAGGCAGC973100.9791185437767411No Hit
GAATATTGGTGAACGCTACTAATGTTGTTATTAAAGTCTAGGCAGCTCTC899430.9049929008623105No Hit
CTCCCTAGCATTGGTGCCTGGAATATTGGTGAACGCTACTAATGTTGTTA859910.8652284729000694No Hit
CTCCCTAGCATTGTAGGCAGCTCTCTCTCTCGCTACTAATGTTGTTATTA750090.7547292451973033No Hit
GCCTATGCTAGGGAGAGCTGCCTAGACTTTAATAACAACATTAGTAGCGT681460.68567477426996No Hit
GCCTAAGACTTTAATAACAACATTAGTAGCGAGAGAGAGAGCTGCCTACA622350.6261991837626707No Hit
GCATTGGTGCCTGGAATATTGGTGAACGCTACTAATGTTGTTATTAAAGT582360.5859618488889354No Hit
CTCCCTAGCATTGCGCTACTAATGTTGTTATTAAAGTCTAGGCAGCTCTC577620.5811925323772699No Hit
ATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTT519090.5223005291224628No Hit
TTGGTAGACTTTAATAACAACATTAGTAGCGAGGGAGAGCTGCCTAGACT518710.5219181788535951No Hit
ATGCTAGGGAGAGCTGCCTATGCTAGGGAGAGCTGCCTAGACTTTAATAA493850.4969044217903028No Hit
ATTGTAGGCAGCTCTCTCTCTCGCTACTAATGTTGTTATTAAAGTCTTAG451180.4539705113361321No Hit
ATGCTAGGGAGAGCTGCCTAAGACTTTAATAACAACATTAGTAGCGAGAG416330.4189049669412914No Hit
GCATTGTAGGCAGCTCTCTCTCTCGCTACTAATGTTGTTATTAAAGTCTT366530.3687969580212609No Hit
CCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTAAGACTTTAATA359730.36195490057836516No Hit
GTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTAGA341380.3434914073317274No Hit
ATGCTAGGGAGAGCTGCCTAGACTTTAATAACAACATTAGTAGCGTTCAC340650.34275689234153417No Hit
TTGGTAAGACTTTAATAACAACATTAGTAGCGAGGGAGAGCTGCCTAGAC326130.32814708733111564No Hit
GGTAAGACTTTAATAACAACATTAGTAGCGAGGGAGAGCTGCCTAGACTT320390.32237158590137716No Hit
GTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTT315580.3175318364454465No Hit
ACTAATGTTGTTATTAAAGTCTAGGCAGCTCTCCCTCGCTACTAATGTTG302440.3043105666219686No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG297240.2990784050479895No Hit
GGGTAGTAGAGTTGAATAGAATGGAATGCAATGAAATGGAACGAAGTGGA292710.29452038736911923No Hit
CTACTAATGTTGTTATTAAAGTCTAGGCAGCTCTCCCTCGCTACTAATGT279730.28146010713253294No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC260620.26223191334815976No Hit
CTACTAATGTTGTTATTAAAGTCTAGGCAGCTCTCCCTAGCATTGTAGGC257460.2590523690070494No Hit
CCCAATGCTAGGGAGAGCTGCCTAAGACTTTAATAACAACATTAGTAGCG245650.24716932512460849No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG244490.24600215061964392No Hit
ATATTGGTGAACGCTACTAATGTTGTTATTAAAGTCTAGGCAGCTCTCCC243150.2446538628294262No Hit
AATTAAATGTTGGTGATTATTTTGTGCTGACATCACATACAGTAATGCCA240890.24237988491458146No Hit
GCATTGGGTGCCTGGAATATTGGTGAACGCTACTAATGTTGTTATTAAAG240000.24148438033749653No Hit
CACCAATGCTAGGGAGAGCTGCCTATGCTAGGGAGAGCTGCCTAGACTTT235810.23726846553077108No Hit
GCCTAGACTTTAATAACAACATTAGTAGCGTTCACCAATATTCCAGGCAC232560.23399836454703415No Hit
GTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACATCA232030.2334650865404555No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC231840.2332739114060217No Hit
ATACTAAGCTTGTCCTGTTTATTCTTAACAAAAGCTAGGTTCTCTGCAAG225030.22642179211394522No Hit
CCCAATGCTAGGGAGAGCTGCCTATGCTAGGGAGAGCTGCCTAGACTTTA220680.22204488772032807No Hit
GCCTACAATGCTAGGGAGAGCTGCCTAGACTTTAATAACAACATTAGTAG217710.21905651851365157No Hit
GTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTA208080.20936695775260952No Hit
GTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTAAGACTT204160.20542271287376374No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG202520.20377256960812418No Hit
GCTCTCCCTAGCATTGGTGCCTGGAATATTGGTGAACGCTACTAATGTTG197960.19918436638171172No Hit
CCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTATGCTAGGGAGA195340.1965481618963607No Hit
CATTAGTAGCGAGGGAGAGCTGCCTAGACTTTAATAACAACATTAGTAGC195110.196316739365204No Hit
TTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTGCACTTAATGGCATT191860.19304663838146705No Hit
AGGTACAACAACTTACAAATTAAATGTTGGTGATTATTTTGTGCTGACAT191640.19282527769949098No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGC187510.18866973398784992No Hit
CTCCCTAGCATTGTATTTTATTAAAGTCTAGGCAGCTCTCCCTAGCATTG184670.18581216882052287No Hit
ATCCTAAACTATACATTGTCATTCTATTCCACAAATCCTTGCAGAGAACC182590.18371930419093124No Hit
GTCTCAAGGGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGT175490.17657539127261362No Hit
GCTCTCTCTCTCGCTACTAATGTTGTTATTAAAGTCTTAGGCAGCTCTCC174010.17508623759386574No Hit
TCATAGCACATTGGTAAGACTTTAATAACAACATTAGTAGCGAGGGAGAG171960.17302355851181628No Hit
TCATAGCACATTGGTAGACTTTAATAACAACATTAGTAGCGAGGGAGAGC171380.172439971259334No Hit
ATTCCATTCCATTCGATGTCTTTCCATAACGATCCATTCCATTCTATTCC170480.17153440483306837No Hit
ATTCAATTCTGTTCTACTCGACTCCAATACATTCAATTCCATTCCATCCG170120.17117217826256215No Hit
GTATTTGTACATGGTGCAGAGGGAAAAAGTGGTTAAAATGGAAGGGATGT165990.16701663455092106No Hit
CTACTAATGTTGTTATTAAAGTCTTAGGCAGCTCTCCCTAGCATTGGGTG161740.16274034864911122No Hit
GTTGAATAGAATGGAATGCAATGAAATGGAACGAAGTGGAATCGAGTGGA160280.1612713186687248No Hit
CAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTT159770.1607581643605076No Hit
AGCATAGGCAGCTCTCCCTAGCATTGGTGCCTGGAATATTGGTGAACGCT159100.16008402046539877No Hit
GTCTATGGGTGTGGGGCATGGTATTTGTACATGGTGCAGAGGGAAAAAGT156600.1575685581702165No Hit
ATTCCATTCCATCCGATTCCATTACATTCTTTTAATTTCTTATCCATTTC155360.15632088887180612No Hit
GAATAGAATGGAATGCAATGAAATGGAACGAAGTGGAATCGAGTGGAATG154240.15519396176356445No Hit
CACCAATGCTAGGGAGAGCTGCCTAAGACTTTAATAACAACATTAGTAGC154040.15499272477994988No Hit
GTCTTAGGCAGCTCTCCCTAGCATTGGTGCCTGGAATATTGGTGAACGCT151450.15238670584214106No Hit
TAGCATTGGTGCCTGGAATATTGGTGAACGCTACTAATGTTGTTATTAAA149620.15054538744206764No Hit
GCTAGGGAGAGCTGCCTATGCTAGGGAGAGCTGCCTAGACTTTAATAACA149610.1505353255928869No Hit
TCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAA148440.1493580892387416No Hit
GTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTATGCTAG145760.14666151365830624No Hit
GCCTAGACTTTAATAAAATACAATGCTAGGGAGAGCTGCCTAGACTTTAA142930.14381401034015992No Hit
CTCAAGGGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTG142920.1438039484909792No Hit
CCCTAGCATTGTATTTTATTAAAGTCTAGGCAGCTCTCCCTAGCATTGGT141540.1424154133040386No Hit
ATTCTATTCCTTTCGATTCCATTCCATTCTATTTCATTCCATTCGATTCC140090.1409564451728329No Hit
TGTCTATACTAAGCTTGTCCTGTTTATTCTTAACAAAAGCTAGGTTCTCT139050.13991001285803706No Hit
CCCTAGCATTGTAGGCAGCTCTCCCTAGCATTGGTGCCTGGAATATTGGT136820.13766622049073451No Hit
ATTCTGTTCTACTCGACTCCAATACATTCAATTCCATTCCATCCGATTCC135330.13616700496280587No Hit
GCATTGCGCTACTAATGTTGTTATTAAAGTCTAGGCAGCTCTCCCTCGCT134980.13581484024148036No Hit
CCCTAGCATTGCTCAATTAGAATGGGAGAAATGGAGAATGGTTGACAGTG134450.13528156223490173No Hit
CCCTAGCATTGGGTGCCTGGAATATTGGTGAACGCTACTAATGTTGTTAT133880.13470803683160018No Hit
GGGTTATACAGAGACCCTAATGAAAACAATTGGACTAGAGCGATGAAGGG133460.13428543916600955No Hit
GACTTTAATAACAACATTAGTAGCGTTCACCAATATTCCAGGCACCAATG133020.13384271780205748No Hit
GGTAGACTTTAATAACAACATTAGTAGCGAGGGAGAGCTGCCTAGACTTT129830.1306329879134049No Hit
GGTAGCAGACTTTAATAACAACATTAGTAGCGAGGGAGAGCTGCCTAGAC128420.1292142671789221No Hit
ACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACT128110.12890234985431953No Hit
ATTAAAGTCTAGGCAGCTCTCCCTAGCATAGGCAGCTCTCCCTAGCATTG124630.1254008263394258No Hit
GCGCATCTGTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCC124370.12513921826072685No Hit
GAATGGAATGCACTCGAATGGAAAGGAGTCCAATGGAAGGGAATCGAATG121890.12264387966390605No Hit
CTGGAATATTGGTGAACGCTACTAATGTTGTTATTAAAGTCTAGGCAGCT121810.12256338487046023No Hit
GTAGGCAGCTCTCTCTCTCGCTACTAATGTTGTTATTAAAGTCTTAGGCA121750.12250301377537585No Hit
GTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCA120330.12107423119171233No Hit
GCTAGGGAGAGCTGCCTAAGACTTTAATAACAACATTAGTAGCGAGAGAG118530.11926309833918113No Hit
GAATAGAATGGAATGGATCGTTATGGAAAGACATCGAATGGAATGGAATT117420.11814623308012018No Hit
CCTTTTGATAATTTGCAACATTGCTAGAAAACTCATCTGAGATATTGAGT117010.1177336972637103No Hit
ATTCCATTCTATTCCTTTCGATTCCATTCCATTCTATTTCATTCCATTCG116280.11699918227351708No Hit
GAATGAAATAGAATGGAATGGAATCGAAAGGAATAGAATGGAATGGATCG115940.11665707940137228No Hit
GAATGGAATGCAATGAAATGGAACGAAGTGGAATCGAGTGGAATGGAATC115930.11664701755219158No Hit
GAGTTGAATAGAATGGAATGCAATGAAATGGAACGAAGTGGAATCGAGTG113660.11436297778816608No Hit
GGCTTATACCCAACACTCAATATCTCAGATGAGTTTTCTAGCAATGTTGC112760.11345741136190046No Hit
GTATAAGCCAGTAATTCTAACATAGTGCTCTTGTGGCACTAGTGTAGGTG110750.11143497967657393No Hit
CTATAAAACAAGCATAATCATGAAGATGAATGGAGGAAGTCAGGCTATGT110100.11078095947982655No Hit
CACATACAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCAC108470.10914087806336771No Hit
GTAGTAGAGTTGAATAGAATGGAATGCAATGAAATGGAACGAAGTGGAAT107550.10821518793874063No Hit
GAAGGGTAGTAGAGTTGAATAGAATGGAATGCAATGAAATGGAACGAAGT107520.10818500239119846No Hit
GTCTTAGGCAGCTCTCCCTAGCATTGGGTGCCTGGAATATTGGTGAACAG107080.1077422810272464No Hit
GGTGAACGCTACTAATGTTGTTATTAAAGTCTAGGCAGCTCTCCCTAGCA107060.10772215732888493No Hit
CTACTAATGTTGTTATTAAAGTCTTAGGCAGCTCTCCCTAGCATTGGTGC106510.10716875562394483No Hit
CTAGCATTGTAGGCAGCTCTCTCTCTCGCTACTAATGTTGTTATTAAAGT105610.10626318919767921No Hit
AAGTGGAATCGAGTGGAATGGAATCGAATGGAGTGAAATGGAATGGAATG104340.10498533435172662No Hit
GTATAACCCTTTCAGTTTTTCAGAAAAGGCACTGTTGGGACTGGCAAATC104210.10485453031237714No Hit
ATAGAATGGAATGCAATGAAATGGAACGAAGTGGAATCGAGTGGAATGGA104160.10480422106647351No Hit
GAATCGTACAGAACGGAAAGAAATGGAACGGAATGGAATGCACTCGAATG104120.10476397366975058No Hit
CCAATGCTAGGGAGAGCTGCCTATGCTAGGGAGAGCTGCCTAGACTTTAA104110.10475391182056985No Hit
GTTCTACTCGACTCCAATACATTCAATTCCATTCCATCCGATTCCATTAC103770.10441180894842507No Hit
GAGATATTGAGTGTTGGGTATAAGCCAGTAATTCTAACATAGTGCTCTTG102830.10346599512543656No Hit
CCCTAGCATTGGCAGCTCTCCCTAGCATTGCGCTACTAATGTTGTTATTA101800.10242962465982146No Hit
GAATGGAATGGATCGTTATGGAAAGACATCGAATGGAATGGAATTGACTC101120.10174541891553189No Hit
TTGCGCATCTGTTCACCAATATTCCAGGCACCCAATGCTAGGGAGAGCTG100600.10122220275813397No Hit
TTGGTAGACTTTAATAACAACATTAGTAGCGCAATGCTAGGGAGAGCTGC100070.10068892475155533No Hit
GAAGGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACC99740.10035688372859128No Hit
CACCTACACTAGTGCCACAAGAGCACTATGTTAGAATTACTGGCTTATAC99510.10012546119743451No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGTA39950.0118.820762
GAAGGGT40750.0115.965061
AGGGTAG42150.0112.786373
GTCTCAA27600.0100.839191
GGGTAGT125100.097.1010361
GTAGCAG35850.089.351322
GGTAGTA137900.088.1326142
GGTAGCA37100.086.544011
TCTCAAG32250.085.617612
TCTATGG34600.084.620022
CTATGGG32350.083.5573963
AGCAGAC38450.083.118364
TATGGGT33850.079.6414264
TAGCAGA41550.076.916223
CGGAGAT3200.072.478161
TCAATGT86600.070.377344
ATTGCTC33050.066.654348
TCACTAT24750.065.877319
TGAAGAC5800.064.971923
GGCTCAA97000.063.0634771
Produced by FastQC (version 0.11.5)