FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-50_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-50_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4138605
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGGAGGCCAGCTCCCTGGGCTGGAGAACTGCCTCTGTTGTCCAG252100.6091424525897011No Hit
GGTATATGCAGGCAGAATCATGGACTCATGGAAAGGCTCCTCCATAGGGG221700.5356877498577419No Hit
AAATAGGAGTGGTCCCCAGAGGACAAGGGAAGAAGAAAGTGTGTTTCAAG117470.28383960295800154No Hit
CCCCTATGGAAGAGCCTTTCCATGATTCCCTGAATCCTCAGACCCCCTGA103950.25117159042720916No Hit
GTGCAGGTATATGCAGGCAGAATCATGGACTCATGGAAAGGCTCCTCCAT93580.2261148382123928No Hit
ACATTATTATCACCATTACCATCATTGTTTGACATCATCATTGTCATCAT84020.2030152672216846No Hit
GTATGCAACCAACCACTCTGCAAACTGAACAGTGGCAGGTTCTCACTATC77290.1867537491497739No Hit
GGTATATGCAGCAGGAGAATCAAGGAATACAATAGAGTGTAGAGGAGTCA68450.16539389480271735No Hit
GTTCCTAGGTAGTAGAAATCGTAGGATGCCTCAGTGAAGAAGTCAGGGAT68400.1652730811469082No Hit
GTTCCTAGGTAGTAGAAATGAAGGTCAAAAATGTGAACACGCTGAATCTG67570.16326757446047643No Hit
GTTCCTAGGTAGTAGAATCAAATAGGAGTGGTCCCCAGAGGACAAGGGAA63420.1532400410283175No Hit
TTCCTAGGGTGTGTCATCACCTGCAGGTGGGTGGGCAAGGGGGCTTGCCT62300.1505338151381927No Hit
GTCCTGTAGCAACCAGGGATTAGTATCTGCTTGATTACTTTGTTTATTGA61630.1489149121503502No Hit
CAAGAAAGACGTGGGACCCTGAGACTTGGGAGACAGACAAGTCAGATAGG57670.139346470610266No Hit
AGCCTGCACAGTCAAACAAACCCCTTTTCTTTATAAATTATCCAGCCTCT57640.13927398241678055No Hit
GTCAAACAAACCCCTTTTCTTTATAAATTATCCAGCCTCTGGTATTCCCT56600.13676105837595035No Hit
CCCAAAGACAGTGCAAAAGATGGAATACAGAAATTTTTTTTCTCTGGTCT55370.13378904244304543No Hit
ATCATGGACTCATGGAAAGGCTCCTCCATAGGGGAGGCCAGCTCCCTGGG50220.12134523589470364No Hit
TTTTTATTGTGTCCAAATTAACAATGGTGTACATTTACAAACCAGCAGCT49180.11883231185387347No Hit
CTGATAGACTGAGCAAGATGAAGCCTGGGATTTGACCATTGTATCTGCAA46850.1132023954931674No Hit
TCATTGCACTGCCTCTTTTAATTATTCCTATGACAGATAAAAGCCTTCTC45040.10882894115287639No Hit
GTATTGAGTGGGCAAGAGCTAAAACACTGTATGCAACCAACCACTCTGCA43360.10476960231768917No Hit
CTATGGAAGAGCCTTTCCATGATTCCCTGAATCCTCAGACCCCCTGAGTC42010.1015076336108423No Hit
TCTTTGAATGTATCACTTTTTGTGGAAAATTGGGCATTTTGGATAGTCCT41520.1003236597839127No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA109900.0114.09871
TAGGTAG132550.095.5743946
TTGTAGA42800.087.554621
AGGTATA24950.084.528335
CCTAGGT151150.083.909234
CTAGGTA153300.082.637865
TCCTAGG154600.081.755463
ATTAGCA32400.079.193691
TTCCTAG162550.077.04642
TATATGC63300.076.251853
AGGTAGT173550.073.325247
TTAGCAA36850.069.624392
GTATATG72100.067.249092
GGTATAT75350.066.181631
GGTAGTA192400.063.6554038
CAGGTAT33350.063.4551474
ATATGCA77450.063.0693444
TGTAGAG69900.059.308162
TATGCAG86350.054.7223935
TGCAGGC64750.054.281977
Produced by FastQC (version 0.11.5)