FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-48_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-48_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10074884
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT514420.5105964495472107No Hit
CCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGT457110.4537124199147107No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA444110.4408090455433532No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC418680.41556806013845915No Hit
CTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGT389230.3863369543510377No Hit
GCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTC370230.367478176423669No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC360080.35740361874141674No Hit
CTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTGAA339650.3371254696331987No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA328320.325879682584931No Hit
GTAGCAAAAGCTGCATATGATGGAAGGGAACTAAACTCTGAGGCTATAGC323920.3215123866438561No Hit
GGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTA308460.30616729681453403No Hit
ATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTATGCT308400.3061077427789739No Hit
TTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTTAC301520.2992788800347478No Hit
CAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGA296740.2945344085351255No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG293200.291020720437079No Hit
CTATATGTTAAACCAGGTGGAACCTCATCAGGAGATGCCACAACTGCTTA290710.2885492279613343No Hit
CTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCC281140.27905035928949656No Hit
ATAAAGAACTGACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATG272720.2706929429658942No Hit
GTTGTAAGAGGTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTT266360.2643802151965224No Hit
GTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGA265840.2638640802216681No Hit
TGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGTTAATG262840.26088637844366247No Hit
CTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACT254170.25228082030522636No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT239620.23783896668189927No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC239580.23779926399152584No Hit
TGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTG225840.22416138984826028No Hit
GCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGT213630.21204214361177756No Hit
CCACTAGACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCG211430.20985849564124012No Hit
CCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCT209460.2079031381403498No Hit
CATAAAGTCTGTGTTGTAAATTGCGGACATACTTATCGGCAATTTTGTTA209360.2078038814144163No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG207750.2062058481268866No Hit
GTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTTGATAATGTTG207550.20600733467501958No Hit
AATCAATACTGAGTCCTCTTTATGCATTTGCATCAGAGGCTGCTCGTGTT206840.2053026119208916No Hit
TCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCTATTGAAACAC203830.20231498447029267No Hit
CTCCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAG202130.2006276201294228No Hit
ATGTTAGAATTACTGGCTTATACCCAACACTCAATATCTCAGATGAGTTT197870.19639928360465492No Hit
GCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTT196960.19549604739865986No Hit
GCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTA196510.19504939213195904No Hit
TTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGAATCACCATTAGC187450.1860567327623822No Hit
GCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTAC181610.18026013996786464No Hit
GTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTT176870.17555537115861583No Hit
CATCAGGAGATGCCACAACTGCTTATGCTAATAGTGTTTTTAACATTTGT176490.17517819560006845No Hit
CTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGTCCGCAATTT176430.17511864156450832No Hit
GTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTCTCAGT175620.17431466208444685No Hit
GACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACACAGACTTTGTG173300.17201190604278918No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT172530.17124762925310108No Hit
GACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTC169010.1677537925002412No Hit
GGTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTTGATAATGTT168840.1675850560661542No Hit
GTCCAACATTTTGCTTCAGACATAAAAACATTGTTTTGATAATAAAGAAC168340.16708877243648662No Hit
CCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAAC167650.16640390102754532No Hit
TACCAGTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACC167510.16626494161123842No Hit
ATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGAAAAGCAT164720.1634956789576932No Hit
GTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAA164660.1634361249221331No Hit
GTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTGGTGATTATTT160950.15975370038999953No Hit
GTACCAGGTGGTCCCTGGAGTGTAGAATACTTTTGCATACCAACCTTTTG160950.15975370038999953No Hit
CACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAAT159680.15849313997064382No Hit
GACTTAAAGTTCTTTATGCTAGCCACTAGACCTTGAGATGCATAAGTGCT158660.15748072136612193No Hit
CATAAAAACATTGTTTTGATAATAAAGAACTGACTTAAAGTTCTTTATGC155760.15460227631404988No Hit
GTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGAGTGTGCTC155420.1542648034458759No Hit
ATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTT155090.15393725625029528No Hit
GAATATTGGTGAACAGAAATCAATACTGAGTCCTCTTTATGCATTTGCAT153840.1526965471761263No Hit
GTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCCTACTCATGCCTC153020.15188264202347146No Hit
GTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTT152320.1511878449419368No Hit
GAGTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACTCATTCA151490.15036401411668857No Hit
GTTCAAGGGAACACAACCATCTCTTGCATTGTTGATAATGTTGTTGAGTG149200.148091035092811No Hit
GTGAAAAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTC148810.14770393386167027No Hit
GAATTATGGCCTCACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGC147720.1466220355489949No Hit
CTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTATACATTTG145980.14489496851775166No Hit
CATACAACGTGTTGTAGCTTGTCACACCGTTTCTATAGATTAGCTAATGA144520.14344582031912229No Hit
ATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTG143620.1425525097857206No Hit
CATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGTTGCATGG142830.14176838165084582No Hit
GTAGATAAAAGTGCATTAACATTGGCCGTGACAGCTTGACAAATGTTAAA141860.14080559140929066No Hit
GAAAAGTACTGTGCCCTTGCACCTAATATGATGGTAACAAACAATACCTT141640.1405872266122369No Hit
GTACTTTTCTGTGTCTTTGATTTCGAGCAACATAAGCCCGTTAATACAAA141540.1404879698863034No Hit
CTCTTGAAACTGCTCAAAATTCTGTGCGTGTTTTACAGAAGGCCGCTATA140810.1397633957869887No Hit
GTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAAC140230.1391877067765743No Hit
GTCTCAAGGGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGT139810.13877082852765352No Hit
GTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCC139410.13837380162391943No Hit
TAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCT139090.13805618010093218No Hit
GCTTATGCTAATAGTGTTTTTAACATTTGTCAAGCTGTCACGGCCAATGT137870.13684524804454323No Hit
CACTTGTTCTTGCTCGCAAACATACAACGTGTTGTAGCTTGTCACACCGT133190.13220003327085456No Hit
CTTTCTGTACAATCCCTTTGAGTGCGTGACAAATGTTTCACCTAAATTCA133110.13212062789010773No Hit
GCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGTCAGTC132740.13175337800415368No Hit
GTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACTCATTCACA132450.1314655334989465No Hit
ATGTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTAT130460.12949032465286947No Hit
GTCCTTTAGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAA127420.12647292018449047No Hit
ACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGTAGACATAAACAAGCTTT125630.12469622479028047No Hit
CAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGG125370.12443815730285332No Hit
ACCTTGAGATGCATAAGTGCTATTGAAACACACAACAGCATCGTCAGAGA124790.1238624682924389No Hit
GCATAAGTGCTATTGAAACACACAACAGCATCGTCAGAGAGTATCATCAT124760.12383269127465885No Hit
CTCAAGGGCTGTTGTTTACCGAGGTACAACAACTTACAAATTAAATGTTG124520.12359447513241839No Hit
GCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAAC123850.12292945506866382No Hit
ACATTACAGGTGGTGTTGTTCAGTTGACTTCGCAGTGGCTAACTAACATC123560.12264161056345661No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA123080.1221651782789757No Hit
ACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGC122360.12145052985225438No Hit
TTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGACAC122300.12139097581669427No Hit
GTCAGAGAGTATCATCATTGAGAAATGTTTACGCAAATATGCGTAAAACT122070.12116268534704717No Hit
CAGTAATGCCATTAAGTGCACCTACACTAGTGCCACAAGAGCACTATGTT121800.12089469218702668No Hit
GCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAA121450.12054729364625934No Hit
TGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGA120800.11990212492769148No Hit
CAACAGCATCGTCAGAGAGTATCATCATTGAGAAATGTTTACGCAAATAT120590.11969368580323107No Hit
TGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACAT117670.11679538940597231No Hit
CTATTAGCATAAGCAGTTGTGGCATCTCCTGATGAGGTTCCACCTGGTTT116350.11548520062364985No Hit
CTCAAAGGGATTGTACAGAAAGTGTGTTAAATCCAGAGAAGAAACTGGCC114690.11383753897315345No Hit
CTATAGAGACACTCATAAAGTCTGTGTTGTAAATTGCGGACATACTTATC114680.11382761330056008No Hit
GCCTATACAGTTGAACTCGGTACAGAAGTAAATGAGTTCGCCTGTGTTGT114610.11375813359240662No Hit
AGTAAGGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAACATTGT113850.11300378247531188No Hit
GAACAATACATATGTGAAGCCAATTTAAACTCACCAGACTCATCAAATAA113580.11273578931529137No Hit
CTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAG113010.1121700259774703No Hit
GCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTTGCTCGCAAACATA111720.11088961421292791No Hit
CTCAAGTATTGAGTGAAATGGTCATGTGTGGCGGTTCACTATATGTTAAA111310.11048266163660048No Hit
TCATTATCCAACTTTCTAAGCATAGTGAAAAGCATTGTCTGCATAGCACT111010.11018489145879992No Hit
GTCTCAGTCCAACATTTTGCTTCAGACATAAAAACATTGTTTTGATAATA110570.10974816186469244No Hit
GTAGAATACTTTTGCATACCAACCTTTTGATAATTTGCAACATTGCTAGA110060.1092419525624315No Hit
CCATTAGCAACAGCCTGCTCATAAGCTTCTTGAGCAGTAGCAAAAGCTGC108970.10816005424975612No Hit
GTTTATGAAAAACTCAAACCCGTCCTTGATTGGCTTGAAGAGAAGTTTAA108310.1075049598585949No Hit
GTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGAAAAGCATTG107690.10688956815780708No Hit
GGTCAGTCTCAGTCCAACATTTTGCTTCAGACATAAAAACATTGTTTTGA106810.1060161089695921No Hit
ATTTACAACACAGACTTTATGAGTGTCTCTATAGAAATAGAGATGTTGAC106750.105956554934032No Hit
GAAATAGAGATGTTGACACAGACTTTGTGAATGAGTTTTACGCATATTTG105490.10470592018726964No Hit
ATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTT105110.10432874462872228No Hit
CTCTTGCATTGTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTA104650.10387216368942809No Hit
ACATAAGCCCGTTAATACAAACTGGTGTACCAACCAATGGAGCTTCAACA104590.10381260965386797No Hit
CTCTATTTCTATAGAGACACTCATAAAGTCTGTGTTGTAAATTGCGGACA103200.10243294116339205No Hit
GTATAGGCAGAGCACTTCTCATTAAGTACTTTATCAATCCTTTCATCAAG103040.10227413040189842No Hit
AATTAAATGTTGGTGATTATTTTGTGCTGACATCACATACAGTAATGCCA102950.10218479934855826No Hit
GTGCTATTGAAACACACAACAGCATCGTCAGAGAGTATCATCATTGAGAA102550.10178777244482418No Hit
CCCTAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAG102450.10168851571889066No Hit
GCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTATACAT102140.10138081986849676No Hit
GCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGA101830.10107312401810284No Hit
GTGATAGAGCCATGCCTAACATGCTTAGAATTATGGCCTCACTTGTTCTT101720.10096394161957596No Hit
CTTGTAACCTTGCACTTCTATCACAGTGTCATCACCAAAAGTAACCTTTG101500.10074557682252223No Hit
CTATAGATTAGCTAATGAGTGTGCTCAAGTATTGAGTGAAATGGTCATGT101370.10061654307880864No Hit
GTTAATGCACTTTTATCTACTGATGGTAACAAAATTGCCGATAAGTATGT101340.1005867660610286No Hit
GTCTGTGTCAACATCTCTATTTCTATAGAGACACTCATAAAGTCTGTGTT101250.10049743500768842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAG39900.0113.884362
GCTCAAT47600.0112.818612
AGGGTAG12950.0111.362733
TCAATGT48700.0110.565224
AAGGGTA13300.0108.4353562
GTCTCAA22600.0101.981281
CCCTAGC130150.099.903351
GGGTAGT45000.097.441661
CCTAGCA134200.097.097182
GTCGAGA502.2773747E-886.9640965
GAAGGGT16700.086.799121
TAGCATT167400.078.011394
GCTCTCC7250.076.975861
TGCGCAT9150.075.242154
GGGCGTC406.676487E-572.4772641
ACCGATG406.679936E-572.46973
CTCTCCC7700.071.530672
GGTGAAC38400.070.765948
GGTAGTA63800.069.06412
TCGAGAG651.4033503E-766.8954546
Produced by FastQC (version 0.11.5)