FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-48_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-48_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12532161
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA1465411.169319481292971No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC817740.6525131619359182No Hit
GTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTT646840.5161440233651642No Hit
CACTTAGATGAACCTGTTTGCGCATCTGTTATGAAATAGTTTTTAACTGT627750.5009112155517313No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG581920.46434130554179764No Hit
GTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGTC558220.44542996215896047No Hit
TAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCC543330.4335485316538784No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC520440.41528352532336604No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT499150.3982952341579397No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC467030.3726651772188372No Hit
GGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAATTACAATCTAGT438820.3501550929644137No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG414510.3307570019248875No Hit
GTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCA376340.30029936576780336No Hit
ATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTC364310.2907000636203126No Hit
ATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCATCTGTTA361680.2886014630677024No Hit
TTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATG337360.26919539255839436No Hit
TCATTATGCCTTTAGGTAATGTTGCACTATCACCATAATTTTGAAGGTCA333220.2658918920687342No Hit
TTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTATGC330500.2637214762880879No Hit
GTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAA325370.25962800829003074No Hit
GTCAAATGGAAATTGATTTCTTAGAATTAGCTATGGATGAATTCATTGAA318040.25377905693998026No Hit
GTTTAAATATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGC317530.25337210398110904No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC316960.2529172742035472No Hit
TTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAG303670.24231255886355113No Hit
GTCTTAAAACAGCTGTACCTGGTGCAACTCCTTTATCAGAACCAGCACCA293060.23384634142507424No Hit
AGGTAATGTTGCACTATCACCATAATTTTGAAGGTCACACTTTTCTAATA286470.22858787083887608No Hit
GCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCGAC282790.22565142595917814No Hit
GAATTAGCTATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGC276890.2209435387879233No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT271880.21694582442724764No Hit
GTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAATTCAAAAGGTG266430.21259701339617323No Hit
ATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTG259390.2069794666697946No Hit
ATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACA256850.20495268134522052No Hit
CTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACAC256710.20484096876827546No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC253440.20223168214963086No Hit
GCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGACCTTC252820.20173695502316No Hit
TTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGT243710.1944676580519513No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG230590.18399859369824567No Hit
GTAATAAATCAATAACAGAACACACACACTTAGATGAACCTGTTTGCGCA227130.18123769715374705No Hit
CCTTTAGGTAATGTTGCACTATCACCATAATTTTGAAGGTCACACTTTTC217670.17368911874017576No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG214540.17119154469847617No Hit
CATTTATGCTTTGGTGTAAAGATGGCCATGTAGAAACATTTTACCCAAAA213180.17010633680815304No Hit
ATGTTGCACTATCACCATAATTTTGAAGGTCACACTTTTCTAATAGCATT209200.1669305078350015No Hit
GTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATTTCATTTAT203060.16203111338898374No Hit
TATTGACACAGTTGAGTATATTTTGCGACATTCATCATTATGCCTTTAGG201510.16079429557280664No Hit
ATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAG200060.15963727245444742No Hit
TATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGAAATT197660.1577221997068183No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA193980.1547857548271204No Hit
CCATAATTTTGAAGGTCACACTTTTCTAATAGCATTCTTTGCATTTTGTA193940.15475383694799325No Hit
TCCCTATGGTGCTAACAAAGACGGCATCATATGGGTTGCAACTGAGGGAG189180.15095560933186225No Hit
ACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACG188160.1501417034141199No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA187650.1497347504552487No Hit
CCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTGGT185870.14831440483409047No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC185510.1480271439219461No Hit
ATGCAAAGAATGCTATTAGAAAAGTGTGACCTTCAAAATTATGGTGATAG185080.14768402672132921No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG182640.145737036094573No Hit
CAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTTGCG180520.14404538850083398No Hit
GTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACCGGG176810.14108500521179068No Hit
ATTCATCATTATGCCTTTAGGTAATGTTGCACTATCACCATAATTTTGAA175770.14025514035448478No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC175020.13965668012085067No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA174740.1394332549669606No Hit
GTTATGAAATAGTTTTTAACTGTACTGTCCATAGGAATAAAATCTTCTAA170960.1364170153894448No Hit
CTATTGACTATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCAT170940.1364010564498812No Hit
GTATAACTCTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATAT170750.13624944652402726No Hit
CATTAACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCT169560.1352998896199945No Hit
GAATAAAATCTTCTAATTCAAAAGGTGATTCCTTAAAACGTTTAGCTAGT167420.1335922830866919No Hit
ATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTAT167370.13355238573778297No Hit
GGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCATAGTCAGTT165730.13224375269356978No Hit
CTATAATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTG164400.131182483212592No Hit
ATTACAATCTAGTCAAGCGTGGCAACCGGGTGTTGCTATGCCTAATCTTT163730.1306478587372122No Hit
GCTATGGATGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGA163330.1303286799459407No Hit
CATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTT163080.130129193201396No Hit
GTCATTAAGATCTGAATCGACAAGCAGCGTACCCGTAGGCAACCACTGTC162920.13000152168488738No Hit
TGAGTATATTTTGCGACATTCATCATTATGCCTTTAGGTAATGTTGCACT161230.12865299129176525No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC160520.12808644893725832No Hit
CCATAGGGAAGTCCAGCTTCTGGCCCAGTTCCTAGGTAGTAGAAATACCA160050.12771141385751428No Hit
ACGTAATGCGGGGTGCATTTCGCTGATTTTGGGGTCCATTATCAGACATT159720.12744809135471527No Hit
GTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATTTT159480.12725658407995238No Hit
CCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGA158820.12672993907435437No Hit
GATGAATTCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATAT157160.1254053470905776No Hit
GGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAGTTGAGTATATT157160.1254053470905776No Hit
TGACTATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCCATGTAG156470.12485476367563424No Hit
ATATGAGAGTTATACATTTTGGTGCTGGTTCTGATAAAGGAGTTGCACCA153750.12268434789498793No Hit
GACTATTGACTATACAGAAATTTCATTTATGCTTTGGTGTAAAGATGGCC152520.12170287311182805No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC151870.12118420757601184No Hit
ATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCC151710.12105653605950321No Hit
TCATTGAACGGTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTAT151030.12051393211434167No Hit
GTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGGAACGC148230.11827968057544105No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG146460.11686731442406462No Hit
TCCCAAGATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTA145590.11617310055304907No Hit
ACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTGTCAAT145000.1157023118359236No Hit
CTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTC144790.11553474297050605No Hit
ATCTAGTCAAGCGTGGCAACCGGGTGTTGCTATGCCTAATCTTTACAAAA144550.11534323569574313No Hit
GGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGG144460.11527142046770704No Hit
GTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCC143700.11466498076429117No Hit
GGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC143230.11428994568454713No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT143060.11415429469825673No Hit
AACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCA142960.11407450000043887No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC141750.11310898415684255No Hit
AACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCA141170.11264617490949885No Hit
CCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGA140390.11202377626651941No Hit
CTCATATTATAGGGTACAGCTAATGTTAATGTGTTTAAATATTGACACAG139120.11101038360423233No Hit
ATCTAAGTGTGTGTGTTCTGTTATTGATTTATTACTTGATGATTTTGTTG138880.11081887632946943No Hit
AACATATCGTTTATGGAGATTTTAGTCATAGTCAGTTAGGTGGTTTACAT138440.11046777965907077No Hit
ACATTACCTAAAGGCATAATGATGAATGTCGCAAAATATACTCAACTGTG137300.10955812010394696No Hit
CCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTG136850.10919904396376649No Hit
AATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTAGTCAT136330.10878411153511353No Hit
GGTATATGCGCTAGTTATCAGACTCAGACTAATTCTCCTCGGCGGGCACG136210.10868835789773208No Hit
CCATCAAAGTTGAATCAGCATCAGAGACAAAGTCATTAAGATCTGAATCG134260.10713236129028345No Hit
AACATTAGCTGTACCCTATAATATGAGAGTTATACATTTTGGTGCTGGTT133860.10681318249901194No Hit
GCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTC133800.1067653056803212No Hit
GGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACC132130.10543273422676264No Hit
CACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTC131040.10456297202054776No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA130850.10441136209469382No Hit
ATACTCAACTGTGTCAATATTTAAACACATTAACATTAGCTGTACCCTAT129940.10368523034455111No Hit
GCTATGCCTAATCTTTACAAAATGCAAAGAATGCTATTAGAAAAGTGTGA129890.10364533299564217No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA129000.10293516018506305No Hit
CAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCA128580.10260002245422797No Hit
TGTAGAAACATTTTACCCAAAATTACAATCTAGTCAAGCGTGGCAACCGG127350.10161854767106807No Hit
GATTTATCTGTAGTTTCTAAGGTTGTCAAAGTGACTATTGACTATACAGA126950.10129936887979656No Hit
TCTTTACACCAAAGCATAAATGAAATTTCTGTATAGTCAATAGTCACTTT125720.10031789409663665No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTAT18800.081.332874
TCAAATG59050.080.140192
TGGAAAT60950.078.586597
GCAGGTA20250.075.151473
CTCGGAC608.0643076E-872.467483
TATGCGC35350.068.469715
ATGCGCT36500.066.311926
TGCGCTA37100.065.236897
AATGGAA74600.064.1130755
CTCGTGG22200.063.6614951
CGCTAGT37900.063.4762049
GTATATG39050.063.0979042
ATATGCG38550.062.7861064
TATATGC41850.059.0475853
CTTAACC4350.058.3090362
GCGCTAG41600.058.1788528
TCGTGGG12300.057.163757145
AAATTAG416600.055.5767445
GTTCCTA257100.054.6601641
CGGACTG1206.002665E-1154.35045
Produced by FastQC (version 0.11.5)