FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-46_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-46_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11417284
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGGGAATAGGCTCCTGCTGCACCACACTGTCCCTGCTCTCTTCAACC975880.8547391831542424No Hit
TTCCCATACTGTGCACTCGCTGAGAGCTTGTTTCCCCTGGGACATGAGAG890570.7800191358995712No Hit
CCCTAGCATTGGACTCAGATCTGACTCCAATCCTAGGTAACCTTAGTCAA683250.5984347941244169No Hit
CCCTAGCATTGCACCTGGTATGCTGCAGTGCTGGTCAATGCTGCATATTG632370.5538707804763374No Hit
ATTCCCATACTGTGCACTCGCTGAGAGCTTGTTTCCCCTGGGACATGAGA444770.3895584974500065No Hit
GAGTAAGGTAGGGAGTAAGTCTGGGTCACCCAAGTCATCCTCTCCATTGT441500.3866944187426712No Hit
GGTCAATGCTGCATATTGCATTACTGAAAACACTGCTTCCTCCAGGCCTC423880.37126167659488896No Hit
CTCCCTAGCATTGCACCTGGTATGCTGCAGTGCTGGTCAATGCTGCATAT414510.3630548210940535No Hit
CCCTAGTAGCTGGGACTACAGGCATGTGTCACCACACCTGGCTGATTTTT410600.3596301887559248No Hit
CTCCCTAGCATTGGACTCAGATCTGACTCCAATCCTAGGTAACCTTAGTC409590.35874556505732885No Hit
CCCTAGCATTGACCAGCACTGCAGCATACCAGGTGCAATGCTAGGGGATA407480.3568974898058067No Hit
GTTCAGCACCTGACAGCTCCCCACACTCCCACAGCTCCTGCCTCCTGCAC374860.3283267719363029No Hit
CCTGTAACCCTTGGCTCTCCTGATGTTCTTGCCCAGCTGGGCAGGAAGGT363880.3187097737080027No Hit
GTCCCTGCTCTCTTCAACCCAGGTGGGATCCCTGGCCCAGACAGGAAAAA347390.3042667590645901No Hit
ATGCTGCATATTGCATTACTGAAAACACTGCTTCCTCCAGGCCTCCTGTA340780.2984772910965515No Hit
GTCCAGCAACAGAGGCTCTGCAACTGTGAGTCAGAGTAAGGTAGGGAGTA325180.2848137963459611No Hit
CCCTAGCATTGGGTTAGCAGGTGTGAGCCACCATGCCCAACTCAATGTAC319080.27947101955246095No Hit
GTGCAGCAGAGTGGCCAGCAGACAAGTGGCTGGAGAATGATGTGGGAAAT304030.2662892505783337No Hit
GCATTGCACCTGGTATGCTGCAGTGCTGGTCAATGCTGCATATTGCATTA290590.2545176243316712No Hit
GGGCAACAGAGTGAGACCCTGTCTCTGCAAAAAATTTAAAAATCAGCCAG290220.2541935542638687No Hit
GGGTTAGCAGGTGTGAGCCACCATGCCCAACTCAATGTACCAATTTTTTT288530.2527133423325547No Hit
GCATTGGACTCAGATCTGACTCCAATCCTAGGTAACCTTAGTCAAGCTGC287170.2515221658671187No Hit
GCATATTGCATTACTGAAAACACTGCTTCCTCCAGGCCTCCTGTAACCCT275760.24152854566812915No Hit
GCTCCAAGCAGCCTGGTGACCTTGGATGTCCTTTTTCCTGTCTGGGCCAG274810.2406964738724201No Hit
GTGCTGGTCAATGCTGCATATTGCATTACTGAAAACACTGCTTCCTCCAG267170.23400486490482325No Hit
GTAGTAGAGAGAGGAGGAGAAGTGCTAGCTTCAGTGCTGATGTCTGTAGA266660.2335581737302847No Hit
GTATGCTGCAGTGCTGGTCAATGCTGCATATTGCATTACTGAAAACACTG265890.23288375764323635No Hit
ACTGAAAACACTGCTTCCTCCAGGCCTCCTGTAACCCTTGGCTCTCCTGA264440.23161375332346992No Hit
GAGTGAGACCCTGTCTCTGCAAAAAATTTAAAAATCAGCCAGGTGTGGTG246640.216023355467027No Hit
GAGCAGGGACAGTGTGGTGCAGCAGGAGCCTATTCCCATACTGTGCACTC245680.21518252502083685No Hit
CCCTAGCATTGCTGCTGATTTCCTGCCCTTCCAGCAGTGGGAAGGAAGGA234600.20547794028772515No Hit
CAATGTGTCCAGCAACAGAGGCTCTGCAACTGTGAGTCAGAGTAAGGTAG234590.205469181637244No Hit
CCTCTAGTCTCCTCAAATTGGTGAACTCCTAGTCATCCTTTAAAGCCCAA227530.19928557439755373No Hit
CTACTAGGGAGGCTGAGGTAGGAGGGTCCTTTGAACCCAGGAGGCAGAAG225190.1972360501849652No Hit
CTGCAGTGCTGGTCAATGCTGCATATTGCATTACTGAAAACACTGCTTCC223580.19582590745750042No Hit
CTCAAGACCAGCCTGGGCAACAGAGTGAGACCCTGTCTCTGCAAAAAATT222170.19459093773965858No Hit
ATTCTGGAGGCCCCACTCTGCTCCAAGCAGCCTGGTGACCTTGGATGTCC221010.19357493428384542No Hit
CCCTAGCATTGAGTTGGGCATGGTGGCTCACACCTGCTAACCCAATGCTA217080.1901327846447544No Hit
CTCCCACAGCTCCTGCCTCCTGCACTGTTGTGTCAATTTGGACAGCTGTC216160.18932698880048882No Hit
CCCCTAGGCAGAACTCAAAGCCCTGTCTTGCTGCCTTCAAATGTCTTCAC214230.18763656925762728No Hit
CTCCTGCACTGTTGTGTCAATTTGGACAGCTGTCGTGCCAGCTCTGAAAC213540.18703222237442813No Hit
CCCTAGCATTGGCAGCTCTCCCTTAGGCAGCTCTCCCTAGCATTGGTGCC207950.18213613675546653No Hit
CTCTAGTCTCCTCAGTGGCTGGCAGGTGGCATTGTTCAGCACCTGACAGC203840.17853633140771483No Hit
CATGAAGACAGTGTGGCTCAATAGCTAGCACCGGGGACGGGAACGTGGGT193480.1694623695092458No Hit
TGCTATGGGAGGGCAAGGCAGAAGGATCGCTTGAGCCCAGGCACTCAAGA193000.1690419542861507No Hit
GTTGTGTCAATTTGGACAGCTGTCGTGCCAGCTCTGAAACACACCCAGTG191090.1673690520442515No Hit
CTTCAACCCAGGTGGGATCCCTGGCCCAGACAGGAAAAAGGACATCCAAG187520.16424221382248178No Hit
GGCTCAATGTGTCCAGCAACAGAGGCTCTGCAACTGTGAGTCAGAGTAAG185550.16251675967769566No Hit
GGGTAGTAGAGAGAGGAGGAGAAGTGCTAGCTTCAGTGCTGATGTCTGTA184370.16148323892092026No Hit
GAGTTGGGCATGGTGGCTCACACCTGCTAACCCAATGCTATGGGAGGGCA182360.15972275017420956No Hit
ATGCTATGGGAGGGCAAGGCAGAAGGATCGCTTGAGCCCAGGCACTCAAG174570.1528997614493955No Hit
TTCCTACCCAATTCTGGAGGCCCCACTCTGCTCCAAGCAGCCTGGTGACC172920.15145458412000612No Hit
GATCTGACTCCAATCCTAGGTAACCTTAGTCAAGCTGCTTCCCCTCTAGG170230.14909850714057737No Hit
GTGTGGTGCAGCAGGAGCCTATTCCCATACTGTGCACTCGCTGAGAGCTT167080.14633953223901586No Hit
CTCCTGATGTTCTTGCCCAGCTGGGCAGGAAGGTGGTGCATCTCTCATGT166000.1453935979870519No Hit
CTGCAACTGTGAGTCAGAGTAAGGTAGGGAGTAAGTCTGGGTCACCCAAG165470.14492938951155107No Hit
GACCAGCACTGCAGCATACCAGGTGCAATGCTAGGGGATAACAGATCATG164890.14442138778364452No Hit
CTCATGTCCCAGGGGAAACAAGCTCTCAGCGAGTGCACAGTATGGGAATA164310.14391338605573795No Hit
CTGCAACTTCTGCCTCCTGGGTTCAAAGGACCCTCCTACCTCAGCCTCCC163980.1436243505898601No Hit
GAGTAAGTCTGGGTCACCCAAGTCATCCTCTCCATTGTGGGGAGGAGGAA163690.1433703497259068No Hit
TTCCTTACCTGAGAAAGTTCCTACCTTCACCAATGTCAATTATCCCTCGA161920.14182006859074364No Hit
GCACTGCAGCATACCAGGTGCAATGCTAGGGGATAACAGATCATGGAGAA161150.14114565250369526No Hit
GCACCACACTGTCCCTGCTCTCTTCAACCCAGGTGGGATCCCTGGCCCAG159210.13944647431035262No Hit
GGGTTGAAGAGAGCAGGGACAGTGTGGTGCAGCAGGAGCCTATTCCCATA156980.13749329525305667No Hit
GCATTGGGTTAGCAGGTGTGAGCCACCATGCCCAACTCAATGTACCAATT150380.1317125859354992No Hit
TCCTACCTCAGCCTCCCTAGTAGCTGGGACTACAGGCATGTGTCACCACA149600.13102941119796968No Hit
GTTCAAAGGACCCTCCTACCTCAGCCTCCCTAGTAGCTGGGACTACAGGC148990.13049513351861966No Hit
CTCCAATCCTAGGTAACCTTAGTCAAGCTGCTTCCCCTCTAGGCCTCATT147550.1292338878493344No Hit
GTCCAGGAGAACAGGACTGGCAGCCACAAGGACTAAAGAGCCTCATGGAT147050.12879595532527702No Hit
GTGTCCAGCAACAGAGGCTCTGCAACTGTGAGTCAGAGTAAGGTAGGGAG146900.1286645755680598No Hit
GCAACAGAGGCTCTGCAACTGTGAGTCAGAGTAAGGTAGGGAGTAAGTCT146390.12821788439352128No Hit
GCATTGAGTTGGGCATGGTGGCTCACACCTGCTAACCCAATGCTATGGGA145760.12766608941320895No Hit
CTGTTGCCCAGGCTGGAGCGCAGTGGCGCGATCTCGGCTCACTGCAACTT145700.12761353751032206No Hit
GTCTCAAGGGCTGTTGTTGTGGTTGTGGGTTCCATAGAGGTGGTAGTGGT145040.12703546657856632No Hit
GTCTCAAGGGCTGTTGTTCTGCCCTTTCCTTACCTGAGAAAGTTCCTACC144650.12669387920980157No Hit
CTCCTAGTCATCCTTTAAAGCCCAACTCAAATTCCCCCACCTCTGCAAAG143200.12542387489003515No Hit
GGTAGGGAGTAAGTCTGGGTCACCCAAGTCATCCTCTCCATTGTGGGGAG142690.1249771837154966No Hit
CACCCAGTGTTTCCTACCCAATTCTGGAGGCCCCACTCTGCTCCAAGCAG141020.12351448908514494No Hit
GCTATGGGAGGGCAAGGCAGAAGGATCGCTTGAGCCCAGGCACTCAAGAC139120.12185034549372688No Hit
TTGCATTACTGAAAACACTGCTTCCTCCAGGCCTCCTGTAACCCTTGGCT139060.12179779359083999No Hit
ACTCTGCTCCAAGCAGCCTGGTGACCTTGGATGTCCTTTTTCCTGTCTGG138620.12141241296966951No Hit
AGCATTGGGTTAGCAGGTGTGAGCCACCATGCCCAACTCAATGTACCAAT136680.11971323477632684No Hit
CTCCCTAGCATTGGGTTAGCAGGTGTGAGCCACCATGCCCAACTCAATGT135160.11838191990319238No Hit
CAGCAACAGAGGCTCTGCAACTGTGAGTCAGAGTAAGGTAGGGAGTAAGT133460.11689294932139727No Hit
ATATTGCATTACTGAAAACACTGCTTCCTCCAGGCCTCCTGTAACCCTTG133270.11672653496225546No Hit
ACTCAGATCTGACTCCAATCCTAGGTAACCTTAGTCAAGCTGCTTCCCCT133260.11671777631177432No Hit
CTCCTACCTCAGCCTCCCTAGTAGCTGGGACTACAGGCATGTGTCACCAC132300.11587694586558414No Hit
CCCTTAGGCAGCTCTCCCTAGCATTGGTGCCTGGAATATTGGTGAACAGA130800.11456314829341198No Hit
CTCCCTAGCATTGCTGCTGATTTCCTGCCCTTCCAGCAGTGGGAAGGAAG129960.11382742165299557No Hit
CTCTGCAACTGTGAGTCAGAGTAAGGTAGGGAGTAAGTCTGGGTCACCCA128840.11284645279910704No Hit
CCTCTAGTCTCCTCAGTGGCTGGCAGGTGGCATTGTTCAGCACCTGACAG126880.1111297573048021No Hit
GTGCAATGCTAGGGGATAACAGATCATGGAGAAAGGGCTGCGGCACACAA126100.11044658256727258No Hit
CCTGATGTTCTTGCCCAGCTGGGCAGGAAGGTGGTGCATCTCTCATGTCC125990.11035023741197995No Hit
CCCAATGCTATGGGAGGGCAAGGCAGAAGGATCGCTTGAGCCCAGGCACT125730.1101225124994701No Hit
TGCTAACCCAATGCTATGGGAGGGCAAGGCAGAAGGATCGCTTGAGCCCA125450.10987727028599796No Hit
CCTTAGTCAAGCTGCTTCCCCTCTAGGCCTCATTTCCTCCTCCCCACAAT124230.10880871492729796No Hit
CCCACAGCTCCTGCCTCCTGCACTGTTGTGTCAATTTGGACAGCTGTCGT123960.10857223136430695No Hit
ACCCAATGCTATGGGAGGGCAAGGCAGAAGGATCGCTTGAGCCCAGGCAC123870.10849340350997663No Hit
TCCCCATCCCCTCTGCTCCTCAGTGGCTGGCAGGTGGCATTGTTCAGCAC122950.10768760766571103No Hit
GCCCTATCCCTGGTGATCCATACCCTACTGCTGGCAAAGTATGTGTGGAA121520.10643512064690691No Hit
GCACTGTTGTGTCAATTTGGACAGCTGTCGTGCCAGCTCTGAAACACACC119170.10437683778383722No Hit
CTCAAGGGCTGTTGTTGTGGTTGTGGGTTCCATAGAGGTGGTAGTGGTTG118580.1038600774054495No Hit
CCCATACTGTGCACTCGCTGAGAGCTTGTTTCCCCTGGGACATGAGAGAT118350.1036586284443831No Hit
CTCCCTAGCATTGGCAGCTCTCCCTTAGGCAGCTCTCCCTAGCATTGGTG117030.1025024865808716No Hit
CTGAAAACACTGCTTCCTCCAGGCCTCCTGTAACCCTTGGCTCTCCTGAT116810.10230979627028636No Hit
CTCAAGGGCTGTTGTTCTGCCCTTTCCTTACCTGAGAAAGTTCCTACCTT116220.10179303589189863No Hit
GCATTGACCAGCACTGCAGCATACCAGGTGCAATGCTAGGGGATAACAGA115610.10125875821254862No Hit
CTCCCTCTGTTGCCCAGGCTGGAGCGCAGTGGCGCGATCTCGGCTCACTG115120.10082958433897239No Hit
ATCCTAGGTAACCTTAGTCAAGCTGCTTCCCCTCTAGGCCTCATTTCCTC114590.10036537586347155No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGAAG98800.095.067011
AAGGGTA35050.083.32332
AGACAGT120700.083.214646
AGGGTAG36300.081.051893
GTCTCAA112300.077.3786541
TCTCAAG110950.077.269292
ATGAAGA125400.075.011292
ACGAGGT17000.074.173672
GACGAGG17900.070.449521
GGGTAGT75950.069.3726651
CGAGGTC18700.068.592313
GGTAGTA78150.066.580172
CCCTAGC1088000.058.8850671
CCTAGCA1131450.056.8565252
CTAGCAT1125800.056.8450163
TTGGACT219350.056.6229179
GAAGGGT52300.055.7065351
TCAAGGG155650.055.124714
ATTGGAC234400.053.203758
TAGCATT1230050.052.021364
Produced by FastQC (version 0.11.5)