FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-46_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-46_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11985048
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC2068001.7254832855070752No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC1814961.5143535511914512No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG1502301.2534785008787617No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC1325381.10586123643393No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT704410.5877406581934423No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG676770.564678589522545No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC663010.5531976175648191No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT655650.5470566325641749No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC585230.48830008857703366No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG561460.4684670432692468No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC510320.4257972099903146No Hit
ACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACG470230.392347197941969No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA436440.3641537355545009No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT415490.34667362199967827No Hit
ATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCC379550.3166862577438154No Hit
CTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGG378970.31620232142582994No Hit
AACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCA374400.3123892369892886No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC353430.29489243597522513No Hit
CCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGA338470.28241021646304626No Hit
AACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCA313410.26150083003422264No Hit
GTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGGAACGC309640.25835524396731663No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG306240.2555183758963669No Hit
CTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATT298350.2489351732258394No Hit
TTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCG294350.24559768137766325No Hit
GGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACC288530.24074163073856694No Hit
CCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGA286210.23880588546662476No Hit
GTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCC284190.2371204520832958No Hit
GTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAG283580.23661148457644893No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA276660.23083762367910415No Hit
CTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTC272730.22755853793827108No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC272550.22740835080510313No Hit
ATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGA266680.22251058151790465No Hit
GATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAA262970.21941505782872125No Hit
TTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACC262610.21911468356238542No Hit
GGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGG246090.20533084222941786No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA242330.20219359989213226No Hit
CTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCT241800.20175138222224892No Hit
CTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCGA240810.20092535298982533No Hit
GCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCACCGCTCTCACTC238820.19926495079535766No Hit
ACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCAT231580.19322409055015882No Hit
TCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGG230430.1922645616438082No Hit
CCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAG229670.1916304381926547No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG226420.18891872606601157No Hit
CCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCG223310.1863238261540546No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT222970.18604013934695965No Hit
CTCTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATT219090.18280277225422875No Hit
GGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGC215630.1799158418055564No Hit
CATCTGGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAAT215080.17945693667643217No Hit
GTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTG215070.17944859294681173No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT208100.17363301340136478No Hit
AAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAAT206540.17233139158057606No Hit
CCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCCAATTTGGTCATCTGG206400.1722145793658899No Hit
CTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAA204220.1703956463086339No Hit
CACTCAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTC203960.17017870933850246No Hit
TGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTG201630.16823462033693984No Hit
AAACAACGTCGGCCCCAAGGTTTACCCAATAATACTGCGTCTTGGTTCAC199480.16644071846854513No Hit
GGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC197100.16445491081888033No Hit
GAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTG196230.16372900634190202No Hit
ACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTC194390.16219376009174097No Hit
ACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTTTACCCAATA193380.1613510434000765No Hit
GATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGATCAAA189400.15803023901114122No Hit
CCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAACTA182030.15188091028087664No Hit
CAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCA181920.1517891292550518No Hit
GTTCCTAGGTAGTAGAATGGAATGGAACGAAATGGAATGGAATGGAATGG181750.1516472858515043No Hit
ATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTC179860.15007032095324108No Hit
GAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCAAGGTT176970.1476589830929338No Hit
GATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGG176690.14742535866356146No Hit
ATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGCA176040.14688301623823286No Hit
CTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGA167010.13934862839097517No Hit
AATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACGC166700.13908997277274152No Hit
CTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGT164100.136920603071427No Hit
ATTATATTCCAATCCATTCTGGTTGTTTCCATTCCATTCCATTCCATTTC163470.13639494810533925No Hit
GGACTGCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAG162680.13573579346532447No Hit
CAAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAA162650.13571076227646314No Hit
GAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGAC162620.13568573108760182No Hit
CCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTG160940.13428398451136783No Hit
CCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATG159500.1330824874460244No Hit
CCATTATATTCCAATCCATTCTGGTTGTTTCCATTCCATTCCATTCCATT158810.132506770102214No Hit
GCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAG157910.13175583443637437No Hit
ATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGA156530.13060439974875362No Hit
CTCAGATTCAACTGGCAGTAACCAGAATGGAGAACGCAGTGGGGCGCGAT155460.1297116206793665No Hit
TTATCAGACATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAACACGA153710.12825146799578943No Hit
GTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCA152780.12747550114108847No Hit
GAATAGAATGGAATGGAATGCAATGGAATGGATTCAACTTGAATGGAATG152260.12704162720082557No Hit
GAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAACCTTGGGGC149380.12463863307013873No Hit
GAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGT149290.12456353950355475No Hit
GTAATGGAGTGTAAGGGAATTGAATAGAATCAATCCGAATGTAATGGAAT149100.1244050086407664No Hit
ATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGACCTT149030.1243466025334233No Hit
GTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCC146940.12260276304275126No Hit
ATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTCTTCATAGAACG142600.11898158438748013No Hit
GAATTTAAGGTCTTCCTTGCCATGTTGAGTGAGAGCGGTGAACCAAGACG142560.11894820946899837No Hit
GCCATGTTGAGTGAGAGCGGTGAACCAAGACGCAGTATTATTGGGTAAAC142250.11868955385076473No Hit
TAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAG141670.11820561753277917No Hit
TCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACT137550.11476800092915773No Hit
GAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAA137520.11474296974029641No Hit
TCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGACCCCAAAAT136000.11347472283798946No Hit
GAATAGCAGAAAGGCTAAAAAGCACAAATAGAAGTCAATTAATGAAAGTT133130.11108007243692307No Hit
ATTCTATTCCGTACCATTCCATTCCATTCCATACCATTCCATTCCATTCC132830.11082976054830986No Hit
CACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATG132000.11013723098981332No Hit
AGGTAGTAGAATGGAATGGAACGAAATGGAATGGAATGGAATGGAATGGA129920.1084017352287617No Hit
CACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCA129610.10814307961052805No Hit
GAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTC125340.10458030706259999No Hit
ACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGATGATTCCT124330.10373759037093552No Hit
CCCTTACACTCCATTATATTCCAATCCATTCTGGTTGTTTCCATTCCATT122970.10260284314255563No Hit
CAACATGGCAAGGAAGACCTTAAATTCCCTCGAGGACAAGGCGTTCCAAT121090.10103422197391283No Hit
GTAATGGAGAGTAAGGGAGTTGAATAGAATCAATCCGAATGTAATGGAAT120690.1007004727890952No Hit
AAACCAACCAACTTTCGATCTCTTGTAGATCTGTTCTCTAAACGAACAAA120130.10023322393035054No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA282350.0137.634231
TCCTAGG317500.0123.3220143
TTCCTAG318500.0121.913982
CCTAGGT326900.0119.70944
CTAGGTA334600.0116.9550555
TAGGTAG348900.0112.119076
AGGTAGT445150.088.052537
TGTAGAT460750.066.6568763
TTGTAGA467600.065.3255842
GTAGATC479350.064.070424
TCGGACT2900.062.472864
TAGATCT496750.061.8264435
AGATCTG516500.059.616166
ATCTGTT522700.058.8632558
GATCTGT545700.056.424027
CTTGTAG549450.055.8219341
GTAGAGC26350.055.0045663
TCGTGGG23150.054.167492145
CATGTTT24050.052.441822145
GCAGGTA12750.051.7226263
Produced by FastQC (version 0.11.5)