FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-44_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-44_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7135057
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC596670.8362512030387425No Hit
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC486790.6822510317717153No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT485060.6798263840078642No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG390840.5477741803604372No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG352700.4943198071157666No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA307840.4314471489155588No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC303260.4250281392286004No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA296920.4161424358628109No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT291260.4082097732365698No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT290360.40694839578716746No Hit
GAATAGCAGAAAGGCTAAAAAGCACAAATAGAAGTCAATTAATGAAAGTT230050.3224220913722203No Hit
TTATCAGACATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAACACGA229980.32232398423726677No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT218350.3060241845299904No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC216190.30299687865142494No Hit
TCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACT215260.30169345528704256No Hit
ATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTCTTCATAGAACG211160.29594718023976546No Hit
GAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTG210240.2946577721803764No Hit
ATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGA208480.29219107850154524No Hit
ATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATT202160.28333340574574245No Hit
CTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCT201470.2823663497012007No Hit
GAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGAC197370.2766200746539236No Hit
GTGTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGT187780.26317939716529243No Hit
GTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGTTC183510.25719486193312824No Hit
ATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAA182950.25641000485350013No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC168520.23618591974808328No Hit
ATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTG167220.23436393009894665No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC163130.2286316703566629No Hit
CTGCTATAGTAACCTGAAAGTCAACGAGATGAAACATCTGTTGTCACTTA154390.21638229379246726No Hit
CACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCA153610.21528910000298526No Hit
ATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTAC151540.21238793186935997No Hit
GTATAATTACCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCCTC150520.21095837076003737No Hit
CTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTT145540.2039787488733447No Hit
CTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGA144040.20187645312434083No Hit
ACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGT143820.20156811641448696No Hit
CTCTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACT143690.20138591744957327No Hit
ACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGATGATTCCT141460.19826050443605428No Hit
ACTACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTG140030.19625631582200395No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT139830.19597600972213677No Hit
GTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAA132480.185674760552018No Hit
GTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCC131930.18490391877738327No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG128480.18006863855467448No Hit
GTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCA128000.17939590391499324No Hit
GTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTC127720.17900347537517922No Hit
ACGTAATGCGGGGTGCATTTCGCTGATTTTGGGGTCCATTATCAGACATT126920.1778822509757105No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC126570.17739171530094294No Hit
GATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAG126540.17734966938596286No Hit
GAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAA120800.1693048843197749No Hit
ATTTAGAACCAGCCTCATCCACGCACAATTCAATTAAAGGTGCTGATTTT118550.16615144069626914No Hit
TAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAG118490.166067348866309No Hit
ATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGAT117560.16476392550192662No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC117440.16459574184200632No Hit
CTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACTACCCAATTTA116680.16353057866251103No Hit
GTTGTGATGATTCCTAAGAAAACAAGAAATTTCATGTTCGTTTAGGCGTG115470.16183472675831462No Hit
CACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATG114420.16036311973401193No Hit
AAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCT113340.1588494667947292No Hit
GTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCT112570.15777028831024054No Hit
GCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTT110800.15528957932641604No Hit
CTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAA110750.15521950280144922No Hit
CTGTATAATTACCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCC109680.15371986516715985No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA108140.15156150819818257No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG106980.14993573281895295No Hit
GTTTTAGATTTCATCTAAACGAACAAACTAAAATGTCTGATAATGGACCC104070.14585727906588553No Hit
GTTCAAGTGAGAACCAAAAGATAATAAGCATAATTAAAACAAGGAATAGC103690.14532469747613788No Hit
GTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACA102720.1439652128917821No Hit
ATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAA102010.1429701262372536No Hit
GACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCG99220.1390598561441065No Hit
CCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCA98010.13736400423991008No Hit
GTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTA97870.13716778997000303No Hit
GAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTAC97320.13639694819536832No Hit
GTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTA96950.13587838191061402No Hit
TAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAAT96680.13549996867579334No Hit
ATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCC96440.13516360135595273No Hit
ATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCT96120.13471511159616523No Hit
TAATTAAAACAAGGAATAGCAGAAAGGCTAAAAAGCACAAATAGAAGTCA95880.13437874427632462No Hit
ATTCAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAATATACCATTTA94520.13247266279722783No Hit
CGTCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGA93870.1315616679726595No Hit
GATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAAATTATTCT93480.1310150710779185No Hit
ATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGTCACGC92910.13021619869329704No Hit
TTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCG92550.12971164771353613No Hit
CCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTG92320.1293892956986889No Hit
GGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAAACTACAT92200.1292211120387686No Hit
CTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTA91710.128534362094094No Hit
ATCATAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTC90800.12725896933969835No Hit
TCTCTAGTCAGTGTGTTAATCTTACAACCAGAACTCAATTACCCCCTGCA90520.12686654079988427No Hit
GTTTAGGTGAAACTGATCTGGCACGTAACTGATAGACGTGTTTTACGCCG89840.1259135000603359No Hit
ATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAGAC88820.12448393895101328No Hit
GACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAAC88760.12439984712105313No Hit
GTAATATCTCTGCTATAGTAACCTGAAAGTCAACGAGATGAAACATCTGT87760.12299831662171724No Hit
GTTTAGATGAAATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTC87650.12284414826679031No Hit
ACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGAGAACG87330.12239565850700282No Hit
TAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCAGTAC85900.1203914698929525No Hit
GTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAATTGGGTAG84290.11813500578902172No Hit
ATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAACAGGAAACTGTATAA84060.11781265377417448No Hit
CTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTC83880.11756037828429401No Hit
CTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAAC83860.1175323476743073No Hit
GAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAG83080.1164391538848253No Hit
GTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGGAACGC82860.11613081717497141No Hit
GCAATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATG82570.115724373330164No Hit
CTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTG81750.11457511832070857No Hit
GTCTTTCTTTTGAGTGTGAAGCAAAGTGTTATAAACACTATTGCCGCAAC81430.1141266285609211No Hit
GTACTGAATGGGTGATTTAGAACCAGCCTCATCCACGCACAATTCAATTA81240.11386033776604727No Hit
AACCAGAATGGAGAACGCAGTGGGGCGCGATCAAAACAACGTCGGCCCCA80980.11349593983621994No Hit
CTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTG80580.11293532763648559No Hit
AATTAAAACAAGGAATAGCAGAAAGGCTAAAAAGCACAAATAGAAGTCAA80490.11280918989154537No Hit
CAATTAGATGAAGAGCAACCAATGGAGATTGATTAAACGAACATGAAAAT80440.11273911336657856No Hit
GTCCATTATCAGACATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAA79960.11206637872689734No Hit
GTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCC79620.11158985835712314No Hit
CCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTGGTGGACCCTCAGA79480.11139364408721614No Hit
CTTCGGTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGG79210.11101523085239542No Hit
AACTAAAATGTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCA78890.11056674109260795No Hit
GGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC78530.11006219011284703No Hit
ATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAACACGAACGTCATGA78270.10969779218301971No Hit
ATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGC77790.10902505754333848No Hit
TTTATATACTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATTC77610.10877278205345801No Hit
GATAATAAGCATAATTAAAACAAGGAATAGCAGAAAGGCTAAAAAGCACA77450.10854853717356426No Hit
GGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAATGCAGC77360.10842239942862404No Hit
GCATAATTAAAACAAGGAATAGCAGAAAGGCTAAAAAGCACAAATAGAAG76640.1074132974691022No Hit
CATTAGTAGCGTTATTAACAATAAGTAGGGACTGGGTCTTCGAATCTAAA75070.10521289458514486No Hit
CAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCA74990.105100772145198No Hit
GGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAA74980.10508675684020463No Hit
ATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAG74900.10497463440025778No Hit
GATTTTGGGGTCCATTATCAGACATTTTAGTTTGTTCGTTTAGATGAAAT74650.10462425177542378No Hit
GTCACTAACTGAGAATAAATATTCTCAATTAGATGAAGAGCAACCAATGG74030.10375530286583555No Hit
TCCACGCACAATTCAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAAT72920.10219960401157271No Hit
CATTATTAGTGATATGTACGACCCTAAGACTAAAAATGTTACAAAAGAAA72840.10208748157162584No Hit
CAACTACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGG72510.10162497650684502No Hit
GTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTT71980.10088216534219697No Hit
AATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACAT71870.10072799698727004No Hit
GTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGT71760.10057382863234308No Hit
CAATTCAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAATATACCATT71590.100335568447456No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA71450.0137.436841
TCCTAGG81000.0123.374123
TTCCTAG82300.0119.666772
CCTAGGT83550.0119.088254
CTAGGTA86450.0115.177215
TAGGTAG87800.0113.241196
AGGTAGT105400.094.603557
GCCGGAC259.4057294E-486.961313
CAGGTAT3500.082.82034
TGCGCTA7650.064.413537
TATGCGC7650.063.4684945
ATAGATC2650.062.896544
TCGGACT350.003573691562.1152234
ATGCGCT8000.061.5975956
AGGAGTA4650.057.6604967
TTGTAGA96900.057.2125932
TGTAGAT100350.055.100053
CGCTAGT9150.053.8516659
CGGTACC701.7013044E-551.7637752
GAAGGAG4900.051.7626885
Produced by FastQC (version 0.11.5)