FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-43_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-43_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21657182
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC4952312.286682542539468No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT4899282.262196439038098No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT2946401.3604724751355002No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA1769660.8171238529555692No Hit
ATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGA1657020.7651133928689338No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG1645030.7595771231917431No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA1503650.6942962385410991No Hit
ATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTCTTCATAGAACG1380890.6376129636810551No Hit
TCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACT1377870.6362185070984766No Hit
TTATCAGACATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAACACGA1282940.5923854728653063No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC1233550.5695801051124749No Hit
GAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTG1228950.5674560983972892No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT1196460.5524541466198142No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT1130820.522145494275294No Hit
CTCTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACT1126660.5202246534198217No Hit
CTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGA1097040.5065478971363865No Hit
ATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATT1040250.48032564901564756No Hit
GAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGAC955520.44120236880310654No Hit
GTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAA885880.4090467540975553No Hit
CACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCA881590.4070658869653494No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG843630.3895382141591644No Hit
CTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTT824980.3809267521508569No Hit
AAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCT823300.3801510279592239No Hit
GTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTC803640.37107320795475607No Hit
GTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCA764780.35312996861733903No Hit
GTATAATTACCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCCTC747480.3451418564058796No Hit
ACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGT733790.3388206277252507No Hit
ACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGATGATTCCT714150.32975204253258805No Hit
ATTCAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAATATACCATTTA692720.319856941683364No Hit
AATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACAT681110.3144961334304712No Hit
ACTACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTG678950.3134987737555145No Hit
GCAATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATG666720.3078516863366619No Hit
GTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTA643300.2970377217128249No Hit
CAATTCAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAATATACCATT613370.28321782584640975No Hit
ATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACATATG607530.28052126079930434No Hit
CTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACTACCCAATTTA595530.2749803737162111No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC586210.27067695141500864No Hit
TAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAAT581100.26831745699879145No Hit
CTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTA573470.26479437629512464No Hit
TTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTG561440.2592396369943237No Hit
GTTTAGATGAAATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTC555560.25652460232360796No Hit
CTCCTACTCTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCA554850.25619676650452494No Hit
CTGTATAATTACCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCC545740.2519903097272766No Hit
CGTCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGA541240.24991247707111663No Hit
ATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAACAGGAAACTGTATAA540100.24938609279822277No Hit
TAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAG533130.24616776088412612No Hit
ATTTAGAACCAGCCTCATCCACGCACAATTCAATTAAAGGTGCTGATTTT532110.2456967854820632No Hit
CACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATG530260.24484256539008628No Hit
GACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCG529280.2443900596116337No Hit
GTTGTGATGATTCCTAAGAAAACAAGAAATTTCATGTTCGTTTAGGCGTG527030.2433511432835537No Hit
GCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTT507510.234337966961722No Hit
CTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTG506030.23365459088814047No Hit
ATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAA505530.2334237205930116No Hit
GTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTT495380.22873705360189522No Hit
ATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTG494920.22852465293037666No Hit
ATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGC487680.22518165105691038No Hit
ATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTAC479190.2212614734456219No Hit
GAATAGCAGAAAGGCTAAAAAGCACAAATAGAAGTCAATTAATGAAAGTT477860.22064735846057904No Hit
AATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAATATACCATTTAGAAT473550.21865725651656803No Hit
GAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTTATAAC459370.21210977494671282No Hit
GTGTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGT454710.2099580637961116No Hit
CTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCT454480.2098518634603523No Hit
ACAGGAAACTGTATAATTACCGATATCGATGTACTGAATGGGTGATTTAG453910.2095886713239054No Hit
ACGTAATGCGGGGTGCATTTCGCTGATTTTGGGGTCCATTATCAGACATT443390.20473116031439362No Hit
ACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAACAGGAAACTG440120.2032212685842507No Hit
GTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGTTC437680.20209462154402175No Hit
CAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAATATACCATTTAGAA429890.1984976623459137No Hit
CTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTTATAACACTTTGCTTC421900.1948083550297541No Hit
ATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAG418780.19336772438814986No Hit
ATTTTGGGGTCCATTATCAGACATTTTAGTTTGTTCGTTTAGATGAAATC415650.19192247634064302No Hit
GAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTT413080.19073580302368054No Hit
GGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAAACTACAT412410.19042643682820784No Hit
ATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAACACGAACGTCATGA410870.18971535631921088No Hit
GTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGT408540.18863950074391025No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC395150.1824567942403587No Hit
GTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAA394090.18196734921468544No Hit
CCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTG388840.17954321111583216No Hit
ATCATAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTC384510.177543874360016No Hit
GTACTGAATGGGTGATTTAGAACCAGCCTCATCCACGCACAATTCAATTA380880.17586775601738028No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC378960.17498121408408537No Hit
GTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCC374310.17283412033938672No Hit
GTTCAAGTGAGAACCAAAAGATAATAAGCATAATTAAAACAAGGAATAGC373840.17261710226196555No Hit
TTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTT366830.16938030072425858No Hit
ACTTTACTCTCCAATTTTTCTTATTGTTGCGGCAATAGTGTTTATAACAC364320.16822133184271157No Hit
GAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTAC363010.1676164516694739No Hit
ATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATT361540.16693769300179498No Hit
ATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTGTCACGC359470.16598188997996138No Hit
CTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTTG359210.16586183742649435No Hit
ACTCTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAAC355800.16428730201371536No Hit
GTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTC350410.16179852023222596No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC347860.16062108172706865No Hit
GATTTTGGGGTCCATTATCAGACATTTTAGTTTGTTCGTTTAGATGAAAT344640.15913427702643862No Hit
CATTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTT333100.1538057906148639No Hit
GGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAATGCAGC326150.1505966935125724No Hit
GTTGAGTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTT324360.149770177856011No Hit
CATCACAACTGTAGCTGCATTTCACCAAGAATGTAGTTTACAGTCATGTA324230.14971015157927747No Hit
ATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCT323810.14951622053136923No Hit
GTCCATTATCAGACATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAA322560.14893904479354703No Hit
TCCACGCACAATTCAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAAT314580.14525435488328997No Hit
GACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAAC312100.1441092382194507No Hit
GGTAAACAGGAAACTGTATAATTACCGATATCGATGTACTGAATGGGTGA309940.14311187854449392No Hit
CAACTACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGG305940.14126491618346285No Hit
ATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGTATA305280.14096016739389272No Hit
GTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGATGATTCCTAAG304610.14065080119842No Hit
TGATTGAACTTTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCTG301520.13922402277452348No Hit
TTCATTAATTGACTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTG300140.13858682075996775No Hit
AGAATGTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATG299100.13810661054609968No Hit
GCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACT298780.13795885355721718No Hit
ACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTA297830.1375201999964723No Hit
ATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAATATACCATTTAGAATA297000.13713695530655834No Hit
GAGTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTG294380.135927194960083No Hit
TTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGG288960.13342456096088587No Hit
GCGTAAAACACGTCTATCAGTTACGTGCCAGATCAGTTTCACCTAAACTG282180.13029395975893818No Hit
GGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC281760.13010002871102988No Hit
CATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAACACGAACGTCATG280850.12967984477389533No Hit
CTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTT280050.1293104523016891No Hit
GAATGTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGA278860.12876098099928235No Hit
AAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATT276110.12749119437607348No Hit
GCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAAC272940.12602747670495634No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT271470.12534871803727743No Hit
GTAAAAGGTAAACAGGAAACTGTATAATTACCGATATCGATGTACTGAAT267400.1234694338349283No Hit
CAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAACAGGAAACTGTAT267360.12345096421131799No Hit
CTTCTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGC267130.1233447638755587No Hit
GATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAG266300.12296151918564475No Hit
CTACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGA262830.12135927933745028No Hit
GGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAA260020.12006178827882594No Hit
GTTTAGGTGAAACTGATCTGGCACGTAACTGATAGACGTGTTTTACGCCG257510.11890281939727892No Hit
ACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACTACCCAAT257420.11886126274415572No Hit
AGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTG255570.11800704265217885No Hit
GTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACA255360.11791007712822472No Hit
GATCAGTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTT254850.11767458942719325No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG253170.1168988652355602No Hit
GAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTGGATGAG251580.11616469769705033No Hit
CCACTGCGTTCTCCATTCTGGTTACTGCCAGTTGAATCTGAGGGTCCACC249610.11525506873424252No Hit
CAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCA249380.11514886839848323No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA247570.11431311793011667No Hit
CGGCAATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAA247040.11406839541728005No Hit
ACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTA245090.1131680012662774No Hit
GAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAA243910.11262314736977322No Hit
TCGATGTACTGAATGGGTGATTTAGAACCAGCCTCATCCACGCACAATTC241950.111718135812868No Hit
CCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCA241360.11144570886461591No Hit
ATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGC239490.11058225396083388No Hit
ATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGAT235880.10891537043000331No Hit
GATGAAATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTCTTCAT235860.10890613561819816No Hit
AAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTT233210.10768252305401506No Hit
CTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAA232890.10753476606513257No Hit
AAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCTT232520.1073639220467372No Hit
GTTTCATTATGATCTTGCAGTTCAAGTGAGAACCAAAAGATAATAAGCAT232100.10716999099882893No Hit
CAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTG232010.10712843434570574No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC231220.1067636592794021No Hit
GTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTACAATTA230840.10658819785510415No Hit
GTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCT230760.10655125860788352No Hit
ACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTTCATTA228280.10540614194404424No Hit
ATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAA226250.10446880854582097No Hit
GTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTCACTTC226060.104381077833672No Hit
CTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGATGATTCCTA224170.1035083881180848No Hit
TGCCAGATCAGTTTCACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAG223960.10341142259413068No Hit
CACTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTA223940.1034021877823255No Hit
CCCGTGTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTA222660.10281115982679555No Hit
GTAGTTTACAGTCATGTACTCAACATCAACCATATGTAGTTGATGACCCG222220.10260799396708215No Hit
TTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCG221710.10237250626605068No Hit
CTGCTATAGTAACCTGAAAGTCAACGAGATGAAACATCTGTTGTCACTTA221480.1022663059302914No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG221090.10208622710009085No Hit
TAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTA219270.10124585922582172No Hit
GGTTTATGATGTAATCAAGATTCCAAATGGAAACTTTAAAAGTCCTCATA217760.10054863093453248No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA145900.0136.857511
TCCTAGG168300.0120.39553
CCTAGGT171900.0118.464074
TTCCTAG175350.0113.988152
CTAGGTA181650.0112.30585
TAGGTAG185150.0110.3393866
AGGTAGT223700.091.710287
TCGGACT1150.088.223934
CAGGTAT5550.083.568614
TCCAGGT4600.070.8943943
GACCGAG2100.062.1189962
GCAGGTA7700.060.2346573
CGGACTG2050.060.0972025
CAGACTG63600.058.7965475
TTCACTA36050.058.102511
AAATTAG65450.057.7989655
TTGTAGA223950.057.052212
TTCAGAC65650.056.960283
GACTGTT66750.055.9113667
TGCGCTA9550.055.3941157
Produced by FastQC (version 0.11.5)