FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-42_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-42_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7819787
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTATGACCCTGCTGGGGCAGAAGATCCCCTGGGAGCAATTCACTTGAGA1109501.4188366000250392No Hit
CTCTTGGACATGTCTCCAGCAGGCTGCAGATACCCCTCATTGAGCAGCGA720340.9211759859955265No Hit
TTCCTGGACAACCCTGATGCCTTCTACTACTTTCCAGACAGTGGGTTCTT673890.861775391068836No Hit
TTTCCAGACAGTGGGTTCTTCTGTGAAGAGAATTCCAGTGATGATGATGT622130.7955843298545089No Hit
GTCCAGGAGTGCAGTGGATGGAACTGCTGAAAACCCTTTCCTGGACAACC483190.6179068560307333No Hit
GGATGGAACTGCTGAAAACCCTTTCCTGGACAACCCTGATGCCTTCTACT459010.5869852976813819No Hit
TTCTACTACTTTCCAGACAGTGGGTTCTTCTGTGAAGAGAATTCCAGTGA448100.5730335110150698No Hit
GTCCAGGAAAGGGTTTTCAGCAGTTCCATCCACTGCACTCTTGGACATGT399790.5112543346768909No Hit
GGTCATAGTAGTGCAGGTAGGCAGGGTCTTCTCTCAAGATGAACTTCCTC398360.5094256403659077No Hit
GGAGTGCAGTGGATGGAACTGCTGAAAACCCTTTCCTGGACAACCCTGAT382460.4890926057193118No Hit
CAATGTGTCCAGGGTGACATTACGTCCTGTCCTGCAGATGGGCTGGATGA380910.4871104545430713No Hit
ATTCCTAACAGACTGGTTGGATACCAGCCAATCAATGACGCAGTTACCAT374190.47851687008866095No Hit
TCATTGAGCAGCGATGAAGCAATCATGAGGCCTTCCTGGCGATTCCTAAC361230.4619435286408696No Hit
GGAAAGGGTTTTCAGCAGTTCCATCCACTGCACTCTTGGACATGTCTCCA324750.4152926416026421No Hit
GTCCAGGGTGACATTACGTCCTGTCCTGCAGATGGGCTGGATGAAAGGTG323140.41323376199377293No Hit
GGTTGGATACCAGCCAATCAATGACGCAGTTACCATTTGAGTTGCTCTCC319310.40833593037764326No Hit
CCAATATTCCAGGCACCACCTTTCATCCAGCCCATCTGCAGGACAGGACG313470.40086769626845337No Hit
GTTCCATCCACTGCACTCTTGGACATGTCTCCAGCAGGCTGCAGATACCC313110.4004073256726814No Hit
GGACAACCCTGATGCCTTCTACTACTTTCCAGACAGTGGGTTCTTCTGTG311980.3989622735248415No Hit
CCACTGAAGTCACCACACAGCCTCTCAAGTGAATTGCTCCCAGGGGATCT309670.39600822886863796No Hit
ACCCTGATGCCTTCTACTACTTTCCAGACAGTGGGTTCTTCTGTGAAGAG308790.3948828785234176No Hit
TTATCAAGCAGGGATGTTTACTGAAGCAGGGGCATAGAAGGAAAAACTGG298770.38206923027443074No Hit
TAGTAGTGCAGGTAGGCAGGGTCTTCTCTCAAGATGAACTTCCTCACTTT287090.3671327620560509No Hit
CCTAACAGACTGGTTGGATACCAGCCAATCAATGACGCAGTTACCATTTG279750.35774631713114435No Hit
CTTCAGTGGAGAGCAACTCAAATGGTAACTGCGTCATTGATTGGCTGGTA277220.3545109348886357No Hit
CTTGGACATGTCTCCAGCAGGCTGCAGATACCCCTCATTGAGCAGCGATG275770.352656664433443No Hit
GTTGGATACCAGCCAATCAATGACGCAGTTACCATTTGAGTTGCTCTCCA264660.33844911632503544No Hit
GTGTCCAGGAGTGCAGTGGATGGAACTGCTGAAAACCCTTTCCTGGACAA255950.32731070552177444No Hit
TCCATCCACTGCACTCTTGGACATGTCTCCAGCAGGCTGCAGATACCCCT255000.3260958386718206No Hit
GCGATGAAGCAATCATGAGGCCTTCCTGGCGATTCCTAACAGACTGGTTG253100.3236661049719129No Hit
GTCTCCAGCAGGCTGCAGATACCCCTCATTGAGCAGCGATGAAGCAATCA245520.31397274631649175No Hit
GGCTCAATGTGTCCAGGGTGACATTACGTCCTGTCCTGCAGATGGGCTGG243230.3110442778044978No Hit
GCTGAAAACCCTTTCCTGGACAACCCTGATGCCTTCTACTACTTTCCAGA242060.3095480733682388No Hit
AGTCACCACACAGCCTCTCAAGTGAATTGCTCCCAGGGGATCTTCTGCCC238080.30445842067053747No Hit
ATCATGAGGCCTTCCTGGCGATTCCTAACAGACTGGTTGGATACCAGCCA236370.30227166034062053No Hit
GCAGATACCCCTCATTGAGCAGCGATGAAGCAATCATGAGGCCTTCCTGG233970.29920252303547396No Hit
GGTCATTATCAAGCAGGGATGTTTACTGAAGCAGGGGCATAGAAGGAAAA230560.2948417904477449No Hit
GCACTACTATGACCCTGCTGGGGCAGAAGATCCCCTGGGAGCAATTCACT227870.29140179905155983No Hit
AATGTGTCCAGGGTGACATTACGTCCTGTCCTGCAGATGGGCTGGATGAA210930.26973880490606716No Hit
GGGTCATAGTAGTGCAGGTAGGCAGGGTCTTCTCTCAAGATGAACTTCCT210490.26917612973345695No Hit
CCCTGATGCCTTCTACTACTTTCCAGACAGTGGGTTCTTCTGTGAAGAGA208780.26698936940354No Hit
GTATCCAACCAGTCTGTTAGGAATCGCCAGGAAGGCCTCATGATTGCTTC203370.2600710223948555No Hit
CTATGACCCTGCTGGGGCAGAAGATCCCCTGGGAGCAATTCACTTGAGAG201220.25732158689232837No Hit
CATGAGGCCTTCCTGGCGATTCCTAACAGACTGGTTGGATACCAGCCAAT200500.2564008457007844No Hit
GTGGATGGAACTGCTGAAAACCCTTTCCTGGACAACCCTGATGCCTTCTA199430.25503252198557325No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCACCTTTCATCCAGCCC198090.25331892032353315No Hit
CTCCAGCAGGCTGCAGATACCCCTCATTGAGCAGCGATGAAGCAATCATG197510.252577212141456No Hit
GTTCACCAATATTCCAGGCACCACCTTTCATCCAGCCCATCTGCAGGACA193170.2470271888479827No Hit
GTTTTCAGCAGTTCCATCCACTGCACTCTTGGACATGTCTCCAGCAGGCT184240.23560744045841658No Hit
GTTTATGGAGTGTGGGAGGGAGGTGTCAGGAGGATGGGGGTGAGGAGGTT181370.2319372637643455No Hit
GCCTCTCAAGTGAATTGCTCCCAGGGGATCTTCTGCCCCAGCAGGGTCAT180120.23033875475124832No Hit
ATAATGACCCCTCTGAATTCTTCTTTCAGAATCACATCATCATCACTGGA177350.22679645877822502No Hit
CATTACGTCCTGTCCTGCAGATGGGCTGGATGAAAGGTGGTGCCTGGAAT175530.22446902965515556No Hit
GTCACCACACAGCCTCTCAAGTGAATTGCTCCCAGGGGATCTTCTGCCCC167790.21457106184605795No Hit
GTGAGGAAGTTCATCTTGAGAGAAGACCCTGCCTACCTGCACTACTATGA167530.21423857197133378No Hit
CAAGTGAATTGCTCCCAGGGGATCTTCTGCCCCAGCAGGGTCATAGTAGT162110.20730743689054446No Hit
GTGGCGACTTCAGTGGAGAGCAACTCAAATGGTAACTGCGTCATTGATTG161220.2061692984732193No Hit
GCCTTCTACTACTTTCCAGACAGTGGGTTCTTCTGTGAAGAGAATTCCAG161140.20606699389638106No Hit
GGGTTCTTCTGTGAAGAGAATTCCAGTGATGATGATGTGATTCTGAAAGA160800.20563219944481864No Hit
ACTTTCCAGACAGTGGGTTCTTCTGTGAAGAGAATTCCAGTGATGATGAT156760.20046581831448862No Hit
GTTTAGCACGCCTCTCTTCAAACAGTTCTCTCCTTTCATTTTCAACCTGC155430.19876500472455322No Hit
GTTCTAGAAAGTGCTTTCCAAAGTTTTATTTTTTTATTTGTACCTGCCTT149480.19115610182221077No Hit
CCATTTGAGTTGCTCTCCACTGAAGTCACCACACAGCCTCTCAAGTGAAT148520.18992844690015215No Hit
CAATGTGTCCAGGAAAGGGTTTTCAGCAGTTCCATCCACTGCACTCTTGG145000.18542704551927056No Hit
ATTCAGAGGGGTCATTATCAAGCAGGGATGTTTACTGAAGCAGGGGCATA142600.18235790821412398No Hit
TCCTAACAGACTGGTTGGATACCAGCCAATCAATGACGCAGTTACCATTT137710.17610454095488792No Hit
CAGCAGTTCCATCCACTGCACTCTTGGACATGTCTCCAGCAGGCTGCAGA136280.17427584664390477No Hit
CTAACAGACTGGTTGGATACCAGCCAATCAATGACGCAGTTACCATTTGA134630.1721658147466165No Hit
CTACTACTTTCCAGACAGTGGGTTCTTCTGTGAAGAGAATTCCAGTGATG133140.1702603920030047No Hit
CCACTGAAGTCGCCACACAGCCTCTCAAGTGAATTGCTCCCAGGGGATCT132110.16894322057621264No Hit
GAATTCCAGTGATGATGATGTGATTCTGAAAGAAGAATTCAGAGGGGTCA131320.16793296287993523No Hit
CCACTGCACTCTTGGACATGTCTCCAGCAGGCTGCAGATACCCCTCATTG130780.16724240698627726No Hit
CCCCTCATTGAGCAGCGATGAAGCAATCATGAGGCCTTCCTGGCGATTCC129880.16609148049684727No Hit
CAATGTGTCCAGGAGTGCAGTGGATGGAACTGCTGAAAACCCTTTCCTGG128630.16449297148375014No Hit
CTTCTGTGAAGAGAATTCCAGTGATGATGATGTGATTCTGAAAGAAGAAT127800.16343156149905363No Hit
CTTGAGAGGCTGTGTGGTGACTTCAGTGGAGAGCAACTCAAATGGTAACT126070.16121922502492717No Hit
AATGGTAACTGCGTCATTGATTGGCTGGTATCCAACCAGTCTGTTAGGAA125370.16032405997759275No Hit
GTTACCATTTGAGTTGCTCTCCACTGAAGTCACCACACAGCCTCTCAAGT125250.1601706031123354No Hit
TCTGTGAAGAGAATTCCAGTGATGATGATGTGATTCTGAAAGAAGAATTC123360.15775365748453252No Hit
GTCATAGTAGTGCAGGTAGGCAGGGTCTTCTCTCAAGATGAACTTCCTCA122740.1569607970140363No Hit
GATGTTTACTGAAGCAGGGGCATAGAAGGAAAAACTGGAAAGTGAGGAAG122610.1567945520766742No Hit
GGCTCAATGTGTCCAGGAAAGGGTTTTCAGCAGTTCCATCCACTGCACTC122460.15660273099510255No Hit
CATGAAGACAGTGTTCTCAAAAGAAGAGAGTGGTCAACCACATCAAGGGC122090.1561295723272258No Hit
GCTTGATAATGACCCCTCTGAATTCTTCTTTCAGAATCACATCATCATCA119810.15321389188733658No Hit
GTAAACATCCCTGCTTGATAATGACCCCTCTGAATTCTTCTTTCAGAATC119800.15320110381523178No Hit
GGCTCAATGTGTCCAGGAGTGCAGTGGATGGAACTGCTGAAAACCCTTTC115940.14826490798278777No Hit
CCACACAGCCTCTCAAGTGAATTGCTCCCAGGGGATCTTCTGCCCCAGCA112160.14343101672718195No Hit
GCGCATCTGTTCACCAATATTCCAGGCACCACCTTTCATCCAGCCCATCT111620.14274046083352399No Hit
CCCTAGCATTGTTCGTTACATGGTCCATCATAGAATTCTCACTGTGATAT110370.14114195182042683No Hit
GTAGTGCAGGTAGGCAGGGTCTTCTCTCAAGATGAACTTCCTCACTTTCC109250.13970968774469178No Hit
CATTGAGCCCACACACTGTACTGTACACACACACTGTACAGTACACACAC108650.1389424034184051No Hit
GTGAAGAGAATTCCAGTGATGATGATGTGATTCTGAAAGAAGAATTCAGA107620.13762523199161308No Hit
GGGTGACATTACGTCCTGTCCTGCAGATGGGCTGGATGAAAGGTGGTGCC106660.13639757706955447No Hit
GAAGAATTCAGAGGGGTCATTATCAAGCAGGGATGTTTACTGAAGCAGGG106230.13584768996904903No Hit
CTTCCTCACTTTCCAGTTTTTCCTTCTATGCCCCTGCTTCAGTAAACATC104690.13387832686491333No Hit
CAATAGTACCAAGCTCAAAGGTTGGTACTCAAAACCTGTGGGGATTAAAT103110.1318578114723585No Hit
TTACTGTTCTGTCCCCACTCTCTTATCTCCCACTCACTCCTCAATCCACT102410.13096264642502412No Hit
GGGATGTTTACTGAAGCAGGGGCATAGAAGGAAAAACTGGAAAGTGAGGA102310.13083476570397634No Hit
GTTAGGAATCGCCAGGAAGGCCTCATGATTGCTTCATCGCTGCTCAATGA102190.130681308838719No Hit
GCTCCCAGGGGATCTTCTGCCCCAGCAGGGTCATAGTAGTGCAGGTAGGC102140.13061736847819513No Hit
GCACTCTTGGACATGTCTCCAGCAGGCTGCAGATACCCCTCATTGAGCAG102000.13043833546872824No Hit
GTCCTAGGCTAGAGGGGGTATCCCTAGTTAGACTTTGGGAAGCCACCTTC101810.13019536209873747No Hit
TGTCTCCAGCAGGCTGCAGATACCCCTCATTGAGCAGCGATGAAGCAATC101150.12935134933982217No Hit
GTCTGTTAGGAATCGCCAGGAAGGCCTCATGATTGCTTCATCGCTGCTCA100810.12891655488825976No Hit
CTTCTGCCCCAGCAGGGTCATAGTAGTGCAGGTAGGCAGGGTCTTCTCTC100400.12839224393196386No Hit
TTGCGCATCTGTTCACCAATATTCCAGGCACCACCTTTCATCCAGCCCAT100320.12828993935512567No Hit
GGTTTTCAGCAGTTCCATCCACTGCACTCTTGGACATGTCTCCAGCAGGC99800.12762495960567724No Hit
GTATAGGATTGCTTGAATGGCTGCTGTGTTGGCATCTGCTCGGGCGTATC99680.1274715027404199No Hit
GCTCTCCACTGAAGTCACCACACAGCCTCTCAAGTGAATTGCTCCCAGGG97680.12491388831946446No Hit
CTTCTACTACTTTCCAGACAGTGGGTTCTTCTGTGAAGAGAATTCCAGTG97540.12473485530999756No Hit
GTCTTCTCTCAAGATGAACTTCCTCACTTTCCAGTTTTTCCTTCTATGCC97170.12426169664212082No Hit
GTTCTTCTGTGAAGAGAATTCCAGTGATGATGATGTGATTCTGAAAGAAG97110.12418496820949215No Hit
TCTGTTCACCAATATTCCAGGCACCACCTTTCATCCAGCCCATCTGCAGG97070.12413381592107303No Hit
GAGAAGACCCTGCCTACCTGCACTACTATGACCCTGCTGGGGCAGAAGAT93390.119427805386515No Hit
ATCCACTGCACTCTTGGACATGTCTCCAGCAGGCTGCAGATACCCCTCAT92520.11831524311339937No Hit
CTACTATGACCCTGCTGGGGCAGAAGATCCCCTGGGAGCAATTCACTTGA92370.11812342203182773No Hit
GTGGGTTCTTCTGTGAAGAGAATTCCAGTGATGATGATGTGATTCTGAAA92350.11809784588761817No Hit
GGTAGGCAGGGTCTTCTCTCAAGATGAACTTCCTCACTTTCCAGTTTTTC92150.11784208444552262No Hit
GAATTGCTCCCAGGGGATCTTCTGCCCCAGCAGGGTCATAGTAGTGCAGG92090.11776535601289397No Hit
GTGGAGAGCAACTCAAATGGTAACTGCGTCATTGATTGGCTGGTATCCAA91150.1165632772350449No Hit
GCAATCATGAGGCCTTCCTGGCGATTCCTAACAGACTGGTTGGATACCAG91130.11653770109083535No Hit
AATATAATGAGGATGAACGTTTATTGAATGAATGAACATTTACTGGCTAT91110.11651212494662579No Hit
GAATTCAGAGGGGTCATTATCAAGCAGGGATGTTTACTGAAGCAGGGGCA91100.11649933687452102No Hit
CTCAAATGGTAACTGCGTCATTGATTGGCTGGTATCCAACCAGTCTGTTA90770.11607733049506334No Hit
TCATGAGGCCTTCCTGGCGATTCCTAACAGACTGGTTGGATACCAGCCAA88340.11296982897360248No Hit
GAACTGCTGAAAACCCTTTCCTGGACAACCCTGATGCCTTCTACTACTTT88080.11263733909887827No Hit
ATCAAGCAGGGATGTTTACTGAAGCAGGGGCATAGAAGGAAAAACTGGAA87990.11252224644993528No Hit
GCCTACCTGCACTACTATGACCCTGCTGGGGCAGAAGATCCCCTGGGAGC87560.11197235934942984No Hit
TTCCAGTGATGATGATGTGATTCTGAAAGAAGAATTCAGAGGGGTCATTA87470.11185726670048686No Hit
ACTCAAATGGTAACTGCGTCATTGATTGGCTGGTATCCAACCAGTCTGTT87390.11175496212364863No Hit
ATGTGATTCTGAAAGAAGAATTCAGAGGGGTCATTATCAAGCAGGGATGT87170.11147362453734354No Hit
CCATTTGAGTTGCTCTCCACTGAAGTCGCCACACAGCCTCTCAAGTGAAT87130.11142247224892443No Hit
CAATGACGCAGTTACCATTTGAGTTGCTCTCCACTGAAGTCACCACACAG87040.11130737959998142No Hit
CCCTCATTGAGCAGCGATGAAGCAATCATGAGGCCTTCCTGGCGATTCCT86980.11123065116735277No Hit
GTGTGGTGACTTCAGTGGAGAGCAACTCAAATGGTAACTGCGTCATTGAT86890.11111555851840978No Hit
TAGTTAGACTTTGGGAAGCCACCTTCAATGTTTTCTGGAACAAGGCAGGT86200.11023318154318013No Hit
ATTCACTTGAGAGGCTGTGTGGTGACTTCAGTGGAGAGCAACTCAAATGG85960.10992626781266548No Hit
GTGTGGCGACTTCAGTGGAGAGCAACTCAAATGGTAACTGCGTCATTGAT85750.10965771829846516No Hit
CAAGATGAACTTCCTCACTTTCCAGTTTTTCCTTCTATGCCCCTGCTTCA85710.10960656601004606No Hit
GTTTACTGAAGCAGGGGCATAGAAGGAAAAACTGGAAAGTGAGGAAGTTC85350.10914619541427406No Hit
ATTACGTCCTGTCCTGCAGATGGGCTGGATGAAAGGTGGTGCCTGGAATA84900.10857073216955909No Hit
TCAGTAAACATCCCTGCTTGATAATGACCCCTCTGAATTCTTCTTTCAGA84680.108289394583254No Hit
GACTGGTTGGATACCAGCCAATCAATGACGCAGTTACCATTTGAGTTGCT84580.10816151386220621No Hit
CATCTGTTCACCAATATTCCAGGCACCACCTTTCATCCAGCCCATCTGCA84500.10805920928536801No Hit
ATGATGATGTGATTCTGAAAGAAGAATTCAGAGGGGTCATTATCAAGCAG83760.10711289194961447No Hit
GTTCTTACTAAACTACTATCTGCATCATTATTACAAAATCCAAGAAATCC83320.1065502167770043No Hit
ATATAAACTCAGACCCAAACATTAATCAGTTCTTCAAATATCTACTCATT83320.1065502167770043No Hit
GTGTCCAGGAAAGGGTTTTCAGCAGTTCCATCCACTGCACTCTTGGACAT82490.10548880679230777No Hit
CAGGAGTGCAGTGGATGGAACTGCTGAAAACCCTTTCCTGGACAACCCTG81210.10385193356289629No Hit
CTGTTCACCAATATTCCAGGCACCACCTTTCATCCAGCCCATCTGCAGGA81170.10380078127447717No Hit
GTCTAAGGGAGAGAATGCAGTCATGGGTTCTTAGCTTCCATCTCTGGTTG80950.10351944368817206No Hit
TTTCCTGACCGTGTGTTCTTGGACAAATAACGTAAGTTCTCTGTGCCTCA80520.10296955658766664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA98000.0109.233171
TAGCACG28050.096.629174
TCCCGGA5300.095.720032
CGGACAA4750.093.069165
AGCACGC29650.091.659215
CCCTAGC137300.089.052191
ACGCCTC30600.088.5699548
GCACGCC31200.086.408236
GTTTGCG46650.086.3819961
TTTGCGC46800.085.326992
CTCCCTA55200.084.293881
CCTAGCA148150.083.015822
CACGCCT32700.082.441917
GACGAGG3550.081.66421
TTGTTCG21000.078.332649
CTAGCAT157800.077.064533
CATGAAG37950.076.965021
CCGGACA6150.075.417894
TAGCATT169150.073.864344
GCATTGT42850.072.3868566
Produced by FastQC (version 0.11.5)