FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-41_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-41_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7698065
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTT887081.152341529981885No Hit
CCCTAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAG854901.1105388172222501No Hit
GTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGT831881.0806351985856186No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCC758030.9847019997882585No Hit
GATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATCTTTTC507540.6593085405228457No Hit
CATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAG449390.5837700772856556No Hit
CAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGA444500.577417831623921No Hit
CCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGG413040.53655041883902No Hit
AGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGC409680.532185685623595No Hit
TTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAA406710.5283275732278176No Hit
GTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACCA382310.49663129630627956No Hit
GTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCA361670.46981936369724075No Hit
CCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCT343010.44557950601872026No Hit
GTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCAT338180.439305202021547No Hit
CAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGA329040.42743208845339703No Hit
GTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAAC322960.41953399977786626No Hit
GGCTCAATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTA322100.4184168359191563No Hit
GCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTA319150.41458470407823267No Hit
CCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACC290440.37728961758571794No Hit
CTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCC283390.36813147199978175No Hit
GCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTT277060.35990862638857946No Hit
TTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGAATCACCATTAGC268890.3492955697308349No Hit
ACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTTTCCAT267810.3478926197687341No Hit
GGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACA264940.3441644101472253No Hit
GCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTAC264770.3439435754309687No Hit
CTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACT260450.3383317755825652No Hit
GGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAA252540.32805646613791906No Hit
TGTATAAACAGGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCT241210.3133384818132869No Hit
CTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGC240150.3119615124060397No Hit
CTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTATACATTTG239430.3110262124313058No Hit
CCCTAGCATTGTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTA226190.2938270851181433No Hit
GTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTT223580.2904366227097329No Hit
GCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGT221450.28766969361781175No Hit
CATTTGGGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGTTGCATGG219860.285604239506941No Hit
ATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTG208550.27091223573716255No Hit
CTCCCTAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTC208000.2701977704786852No Hit
GTGTAAGACGGGCTGCACTTACACCGCAAACCCGTTTAAAAACGATTGTG201820.26216977902888583No Hit
TAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACAAGCT185020.24034611295176128No Hit
ATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCAC183490.23835860050545168No Hit
ACCTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGC182650.23726741720159547No Hit
GTGTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGG181070.2352149533681516No Hit
GTTGATAATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGA180510.23448749783224745No Hit
TGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCT177990.23121394792067876No Hit
CTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGCAT174820.22709602997636422No Hit
GTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTT173930.22593989528537367No Hit
GGCTAGATCTGAGGACAAGAGGGCAAAAGTTACTAGTGCTATGCAGACAA173460.22532935224631126No Hit
GGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATA168820.21930186352024827No Hit
GACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTC168670.21910700935884536No Hit
GCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTATACAT168500.21888617464258875No Hit
GTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAA167270.21728837051908498No Hit
ATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCA166740.21659988581546144No Hit
ATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGC165660.21519693585336058No Hit
AGTAAATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTA159390.20705203190671942No Hit
ATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTT155630.20216768759422013No Hit
CCCTTGGAGAGTGCTAGTTGCCATCTCTTTTTGAGGGTTATGATTTTGGA154400.20056988347071633No Hit
CACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAAT154070.20014120431562998No Hit
TCTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCG151350.1966078488555241No Hit
GCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAAC151270.19650392663610922No Hit
TTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAA151090.19627010164242573No Hit
TGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGA149960.19480220029319056No Hit
AACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACAGATGCG149920.1947502391834831No Hit
GTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAAGCC145620.18916441988993338No Hit
TTGCATGGCTGCATCACGGTCAAATTCAGATTTAGCCACATTCAAAGACT143490.18639749079801224No Hit
GTATAAATACTTCACTTTAGGACCTTTAGGTGTGTCTGTAACAAACCTAC142460.18505949222304566No Hit
GCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAA142170.18468277417766674No Hit
ACTTTCTGTTTTGCTTTCCATGCAGGGTGCTGTAGACATAAACAAGCTTT141300.18355262004152992No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAAC139560.1812923117692563No Hit
GCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGA139410.1810974576078534No Hit
CCATTAGCAACAGCCTGCTCATAAGCTTCTTGAGCAGTAGCAAAAGCTGC138840.1803570117945224No Hit
ACATTTACTTATGCATCAGCATTGTGGGAAATCCAACAGGTTGTAGATGC136220.17695355910868513No Hit
GTCTTTGAATGTGGCTAAATCTGAATTTGACCGTGATGCAGCCATGCAAC136100.1767976757795628No Hit
GGTCATAGCTTGATCAGCCATCTTTTCCAACTTACGTTGCATGGCTGCAT134870.17519987165605902No Hit
GTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGT133670.17364103836483583No Hit
CTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAG132400.17199127313162463No Hit
GTTATAGTCTGGTATGACAACCATTAGTTTGGCTGCTGTTGTAAGAGGTA127300.1653662316439261No Hit
GCTCAAGAAGCTTATGAGCAGGCTGTTGCTAATGGTGATTCTGAAGTTGT125830.1634566608621777No Hit
GTCCATACTAATTTCACTAAGTTGAACAATTTTACTATCTGCATCTACAA125700.16328778725562854No Hit
CAATGACATTCTCTTAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGG124730.16202773034522314No Hit
GGGTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGG124190.1613262553641727No Hit
GACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACCTTACA123280.16014414011832845No Hit
GTTGTAAGAGGTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTT122470.15909192764675278No Hit
GTATTATGTTCAAGGGAACACAACCATCTCTTGCATTGTTGATAATGTTG121720.15811765683973827No Hit
GATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAA121310.15758505546523704No Hit
GAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTG120670.15675367770991802No Hit
CCCTAGCATTGTCATCAGTGCAAGCAGTTTGTGTAGTACCGGCAGCACAA120220.15616911522570934No Hit
CTTGTCCTCAGATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAG120220.15616911522570934No Hit
CCTCAGATCTAGCCTGTTTATACATTTGGGTCATAGCTTGATCAGCCATC120160.15609117356114816No Hit
ATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCTT116720.15162251812630836No Hit
ATGTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGAAAAGCAT116290.15106393619695338No Hit
GAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGC115940.1506092764870133No Hit
GGCTGTTGCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGT115090.1495051029057302No Hit
GTCATAGCTTGATCAGCCATCTTTTCCAACTTACGTTGCATGGCTGCATC113640.14762151267883553No Hit
GTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTGGTC113630.14760852240140865No Hit
GTGTAGTACCGGCAGCACAAGACATCTGTCGTAGTGCAACAGGACTAAGC112390.14599772800047803No Hit
ACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCAC109880.1427371683663362No Hit
GTATAGATAGTACCAGTTCCATCACTCTTAGGGAATCTAGCCCATTTCAA109730.14254231420493332No Hit
CATTCAAAGACTTCTTCAACTTTTTAAGAACAACTTCAGAATCACCATTA108970.14155505312049196No Hit
GTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTAGCCTG108240.1406067628683312No Hit
GACTATAACACATATAAAAATACGTGTGATGGTACAACATTTACTTATGC107700.13990528788728077No Hit
CTTTTGCCCTCTTGTCCTCAGATCTAGCCTGTTTATACATTTGGGTCATA107020.1390219490222543No Hit
AGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCT106710.13861925042202164No Hit
GTGAAAAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTC106610.13848934764775306No Hit
AATGTGTCCAGTTACACAATGACATTCTCTTAGCTAAAGATACTACTGAA105680.13728125184705509No Hit
CTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGC105490.13703443657594475No Hit
CATCTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTG104700.1360082046592228No Hit
ATTTTACTATCTGCATCTACAACCTGTTGGATTTCCCACAATGCTGATGC104060.13517682690390379No Hit
GTGCAAGCAGTTTGTGTAGTACCGGCAGCACAAGACATCTGTCGTAGTGC102910.1336829449998149No Hit
TCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAAT101800.13224102420543343No Hit
TTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAATGTGGCTAAAT101180.1314356270049681No Hit
GCTCATAAGCTTCTTGAGCAGTAGCAAAAGCTGCATATGATGGAAGGGAA101000.13120180201128465No Hit
TTGGTAGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCA100970.1311628311790041No Hit
GTCTTACACCGTGCGGCACAGGCACTAGTACTGATGTCGTATACAGGGCT99410.12913634790041392No Hit
CTTACAAGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTT99100.12873364930018127No Hit
ATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTG99020.12862972708076642No Hit
GCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAG98760.12829197986766802No Hit
AGCTTGATCAGCCATCTTTTCCAACTTACGTTGCATGGCTGCATCACGGT98700.1282140382031069No Hit
AGCTATAGCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTA97140.12618755492451675No Hit
CTTCCATCATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGC97000.1260056910405407No Hit
GTACCAGTGTGTGTACACAACATCTTAACACAATTAGTGATTGGTTGTCC96680.1255900021628812No Hit
CACTGGTACTGGTCAGGCAATAACAGTTACACCGGAAGCCAATATGGATC96040.12475862440756216No Hit
GTAGCAAAAGCTGCATATGATGGAAGGGAACTAAACTCTGAGGCTATAGC96010.12471965357528157No Hit
GTAGTAGAGAGCTAGGCCAATAGCAAAATGACTCTTACCAGTACCAGGTG95680.1242909744201952No Hit
GCCTCAGAGTTTAGTTCCCTTCCATCATATGCAGCTTTTGCTACTGCTCA95540.12410911053621916No Hit
CTAGCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGAT95250.12373239249084023No Hit
GCACCTACCAATGTGCTGAACCTGTTTGCGCATCTGTTCACCAATATTCC94600.12288802445809432No Hit
ATGCTGGACAACAGGGCAACCTTACAAGCTATAGCCTCAGAGTTTAGTTC93810.12186179254137242No Hit
GTGCTATGCAGACAATGCTTTTCACTATGCTTAGAAAGTTGGATAATGAT93080.12091350228921165No Hit
ATGCAGGGTGCTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAA92390.1200171731467583No Hit
GCTAATGGTGATTCTGAAGTTGTTCTTAAAAAGTTGAAGAAGTCTTTGAA91470.1188220676234872No Hit
GTATACGACATCAGTACTAGTGCCTGTGCCGCACGGTGTAAGACGGGCTG91020.1182375051392785No Hit
GCAACAGCCTGCTCATAAGCTTCTTGAGCAGTAGCAAAAGCTGCATATGA90930.11812059264243677No Hit
ATGTTGTACCATCACACGTATTTTTATATGTGTTATAGTCTGGTATGACA90390.11741911766138632No Hit
ATATGCAGCTTTTGCTACTGCTCAAGAAGCTTATGAGCAGGCTGTTGCTA89970.11687352600945822No Hit
GCATTGTCTGCATAGCACTAGTAACTTTTGCCCTCTTGTCCTCAGATCTA89290.11599018714443177No Hit
CAATAAGAGGCCATGCTAAATTAGGTGAATTGTCCATACTAATTTCACTA87360.11348306360104779No Hit
GCTCATTATTCTGTAATTTGACAGCAGAATTGGCCCTTAAAGCTGTTACA86410.1122489872454961No Hit
GATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCTGTTTTGCT85830.11149555115473823No Hit
TCATTATCCAACTTTCTAAGCATAGTGAAAAGCATTGTCTGCATAGCACT85670.11128770671590847No Hit
TGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTGAACA84890.1102744650766134No Hit
ATAAGAGGCCATGCTAAATTAGGTGAATTGTCCATACTAATTTCACTAAG84240.10943009704386752No Hit
CACCAATATTCCAGGCACCTACCAATGTGCTGAACCTGTTTGCGCATCTG84210.10939112621158693No Hit
GAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCTACCAATGTG84150.10931318454702578No Hit
GCTTTTCACTATGCTTAGAAAGTTGGATAATGATGCACTCAACAACATTA82350.106974934610191No Hit
CAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATATTGGTG81690.10611757630001825No Hit
CTCTTAGGGAATCTAGCCCATTTCAAATCCTGTAAATCGGATAACAGTGC80410.10445482078938019No Hit
TAGCTAAAGATACTACTGAAGCCTTTGAAAAAATGGTTTCACTACTTTCT79740.10358447220178058No Hit
TTGCGCATCTGTTCACCAATATTCCAGGCACCTACCAATGTGCTGAACCT79660.10348054998236571No Hit
CCCTAGCATTGTATGTTGAGAGCAAAATTCATGAGGTCCTTTAGTAAGGT78890.10248029862049748No Hit
GTTGTTGAGTGCATCATTATCCAACTTTCTAAGCATAGTGAAAAGCATTG78450.10190872641371565No Hit
ATTGGTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGA77900.10119426115523837No Hit
ATTATCAACAATGCAAGAGATGGTTGTGTTCCCTTGAACATAATACCTCT77670.10089548477442059No Hit
CTGTAGACATAAACAAGCTTTGTGAAGAAATGCTGGACAACAGGGCAACC77470.1006356792258834No Hit
GTGAACAGATGCGCAAACAGGTTCAGCACATTGGTAGGTGCCTGGAATAT77300.10041484450962676No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCTAG47500.0113.05362
GCTCAAT56350.0111.888412
TCAATGT56650.0111.805414
CTCCCTA49050.0109.631651
CCCTAGC194200.0107.625981
CCTAGCA206200.0101.430242
GTCTCAA18500.0101.0692441
GGCTCAA65850.096.519461
CACATTA27100.091.47713145
AGGGTAG8250.090.476263
GGGTAGT24950.088.593191
TAGCATT236450.088.4214864
CTAGCAT273100.076.714543
GGTAGTA29500.074.435552
AAGGGTA10300.072.470252
AGTAGAG35900.061.3663755
CGAAGCC1550.060.780325
GTAGTAG38450.056.354183
TAGAGCC13150.056.211574
TTGTCTC4150.055.8806762
Produced by FastQC (version 0.11.5)