FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-41_A.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-41_A.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7541364
Sequences flagged as poor quality0
Sequence length151
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTAC576750.7647820739059937No Hit
GTTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACC568230.7534843829312575No Hit
TTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACTTT565820.7502886745686854No Hit
GTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACT388010.5145090463741042No Hit
GTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCG346030.45884272394224707No Hit
ATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCACGAATTCGTCTG344820.4572382396606237No Hit
GTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATGATTGAACT343950.4560846022019359No Hit
CTACAAGACTACCCAATTTAGGTTCCTGGCAATTAATTGTAAAAGGTAAA267750.35504187306169016No Hit
GTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCACCAC266840.353835194800304No Hit
ACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTGAAA229540.304374646284147No Hit
ATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGA218570.2898282061441405No Hit
CTTGTAGATCTGTTCTCTAAACGAACAAACTAAAATGTCTGATAATGGAC208430.27638236266012356No Hit
GAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGAC197660.2621011265336085No Hit
ATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATT189100.25075039475617406No Hit
GTATAAATTAGAAGGCTATGCCTTCGAACATATCGTTTATGGAGATTTTA188410.24983544090962853No Hit
CTACCAAGAGTGTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCT176880.23454642953184596No Hit
GAATAGAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTG175960.23332649106978526No Hit
TCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACT171470.22737266096690198No Hit
GTACATGACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGAT167370.2219359786903271No Hit
ATCTAAAACAACACGAACGTCATGATACTCTAAAAAGTCTTCATAGAACG163440.21672471982521996No Hit
GTGTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGT161800.21455004691458998No Hit
CTCTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACT161100.2136218328673699No Hit
TTATCAGACATTTTAGTTTGTTCGTTTAGATGAAATCTAAAACAACACGA160820.21325054724848186No Hit
GTTATAAACACTATTGCCGCAACAATAAGAAAAATTGGAGAGTAAAGTTC157790.20923270644408626No Hit
ATCTAGGACGCTGTGACATCAAGGACCTGCCTAAAGAAATCACTGTTGCT157450.20878185962115076No Hit
ATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCTAAA152870.20270868771219636No Hit
GAATAGCAGAAAGGCTAAAAAGCACAAATAGAAGTCAATTAATGAAAGTT150010.1989162703192685No Hit
ACCATATGTAGTTGATGACCCGTGTCCTATTCACTTCTATTCTAAATGGT148390.1967681178099877No Hit
CTGCTATTCCTTGTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGA145880.19343980744066988No Hit
GTCCTATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAA143610.19042974188754183No Hit
GTATAATTACCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCCTC142020.18832136998028473No Hit
ACGTAATGCGGGGTGCATTTCGCTGATTTTGGGGTCCATTATCAGACATT141510.1876450997458815No Hit
ATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGC141110.1871146917188986No Hit
CACCTAAACTGTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCA140090.18576215125009216No Hit
GTCATGTACTCAACATCAACCATATGTAGTTGATGACCCGTGTCCTATTC138070.18308359071382843No Hit
CTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGCACCTT137270.1820227746598626No Hit
ATTTAGAACCAGCCTCATCCACGCACAATTCAATTAAAGGTGCTGATTTT136880.18150562683355423No Hit
ATTCTGACCAGACCGCTTCTAGAAAGTGAACTCGTAATCGGAGCTGTGAT126480.1677150181319984No Hit
GTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACCAC126290.16746307431918153No Hit
GTCTTGTAGTGCGTTGTTCGTTCTATGAAGACTTTTTAGAGTATCATGAC126160.16729069171041208No Hit
GCTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTT126040.16713156930231718No Hit
ATATTAGAGTAGGAGCTAGAAAATCAGCACCTTTAATTGAATTGTGCGTG124040.1644795291674026No Hit
CCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTCCATTCTG123830.16420106495323658No Hit
TTTATATACTGCTCATACTTTCCAAGTTCTTGGAGATCGATGAGAGATTC121530.1611512187980848No Hit
AAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTGTGCT121290.16083297398189506No Hit
ATTCAGTACATCGATATCGGTAATTATACAGTTTCCTGTTTACCTTTTAC121290.16083297398189506No Hit
CTTTCAGACTGTTTGCGCGTACGCGTTCCATGTGGTCATTCAATCCAGAA120490.1597721579279292No Hit
CTGCTATAGTAACCTGAAAGTCAACGAGATGAAACATCTGTTGTCACTTA114730.1521342823393752No Hit
GACTTGCTTTAGCACTCAATTTGCTTTTGCTTGTCCTGACGGCGTAAAAC114170.15139171110159913No Hit
ACTGTAAACTACATTCTTGGTGAAATGCAGCTACAGTTGTGATGATTCCT112990.14982700742199953No Hit
GTCTGATAATGGACCCCAAAATCAGCGAAATGCACCCCGCATTACGTTTG112060.14859380875926423No Hit
GATAACACTCGCTACTTGTGAGCTTTATCACTACCAAGAGTGTGTTAGAG108870.14436380474407548No Hit
GTTTTAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCA108290.14359471310495026No Hit
ACTACATATGGTTGATGTTGAGTACATGACTGTAAACTACATTCTTGGTG107990.14319690708471305No Hit
CTGTATAATTACCGATATCGATGTACTGAATGGGTGATTTAGAACCAGCC105540.1399481579194427No Hit
CACTTGAACTGCAAGATCATAATGAAACTTGTCACGCCTAAACGAACATG105390.13974925490932408No Hit
GTTTAGGTGAAACTGATCTGGCACGTAACTGATAGACGTGTTTTACGCCG103290.13696461276766378No Hit
GTACTGAATGGGTGATTTAGAACCAGCCTCATCCACGCACAATTCAATTA103260.13692483216564008No Hit
AGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCGTCACC101770.1349490622651287No Hit
TTCCTAGGTAGTAGAAATACCATCTTGGACTGAGATCTTTCATTTTACCG101160.13414019002397976No Hit
ATTCAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAATATACCATTTA100740.13358326159564768No Hit
CTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGACTACCCAATTTA99730.13224398132751583No Hit
GTTCTAAATCACCCATTCAGTACATCGATATCGGTAATTATACAGTTTCC96930.1285311251386354No Hit
GGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCACTGCGTTCTC95370.12646253383340203No Hit
CAATAATACTGCGTCTTGGTTCACCGCTCTCACTCAACATGGCAAGGAAG93380.123823753899162No Hit
GTGTTAGAGGTACAACAGTACTTTTAAAAGAACCTTGCTCTTCTGGAACA93340.12377071309646372No Hit
GAATAAATATTCTCAATTAGATGAAGAGCAACCAATGGAGATTGATTAAA91450.12126453516896943No Hit
GTTCATCAGACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTA91220.12095955055345425No Hit
TAATGAAACTTGTCACGCCTAAACGAACATGAAATTTCTTGTTTTCTTAG91110.12081368834603394No Hit
AATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACAT90130.1195141886799258No Hit
CAGTATTATTGGGTAAACCTTGGGGCCGACGTTGTTTTGATCGCGCCCCA89450.11861249503405484No Hit
GCAATAGTGTTTATAACACTTTGCTTCACACTCAAAAGAAAGACAGAATG89210.1182942502178651No Hit
GCATTACGTTTGGTGGACCCTCAGATTCAACTGGCAGTAACCAGAATGGA87900.11655716392949605No Hit
CTATAGCAGAGATATTACTAATTATTATGAGGACTTTTAAAGTTTCCATT87120.11552286827687937No Hit
CTCTTGGTAGTGATAAAGCTCACAAGTAGCGAGTGTTATCAGTGCCAAGA86690.11495267964787273No Hit
GTTGTGATGATTCCTAAGAAAACAAGAAATTTCATGTTCGTTTAGGCGTG86480.11467421543370669No Hit
CTATTGGTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTC85840.11382556259053403No Hit
CTATTTGTGCTTTTTAGCCTTTCTGCTATTCCTTGTTTTAATTATGCTTA85140.11289734854331392No Hit
CAATTCAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAATATACCATT84700.11231389971363272No Hit
GCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAATGAAACTT84090.11150502747248375No Hit
CTTCTGGAACATACGAGGGCAATTCACCATTTCATCCTCTAGCTGATAAC83480.11069615523133482No Hit
GTTCAAGTGAGAACCAAAAGATAATAAGCATAATTAAAACAAGGAATAGC83050.11012596660232817No Hit
GTAATTATACAGTTTCCTGTTTACCTTTTACAATTAATTGCCAGGAACCT81870.10856126292272857No Hit
ATCATGACGTTCGTGTTGTTTTAGATTTCATCTAAACGAACAAACTAAAA81850.10853474252137943No Hit
GACAAGAGGAAGTTCAAGAACTTTACTCTCCAATTTTTCTTATTGTTGCG81410.1079512936916982No Hit
TCCACGCACAATTCAATTAAAGGTGCTGATTTTCTAGCTCCTACTCTAAT81250.10773913048090504No Hit
CCTTTAATTGAATTGTGCGTGGATGAGGCTGGTTCTAAATCACCCATTCA81190.1076595692768576No Hit
GTAGTAGCCAATTTGGTCATCTGGACTGCTATTGGTGTTAATTGGAACGC81040.10746066626673903No Hit
CTCCTACTCTAATATACCATTTAGAATAGAAGTGAATAGGACACGGGTCA80760.10708938064785097No Hit
GGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAAACTACAT80510.10675787563098665No Hit
CCTATGGACAGTACAGTTAAAAACTATTTCATAACAGATGCGCAAACAGG80270.10643963081479689No Hit
TCATAAACCTCATAATTAAAAATTTATCTAAGTCACTAACTGAGAATAAA79920.10597552379118685No Hit
CTAATAATGAGATCCCATTTATTAGCTGTATGTACAGTTGCACAATCACC79490.1054053351621802No Hit
CCACTGCGTTCTCCATTCTGGTTACTGCCAGTTGAATCTGAGGGTCCACC79340.10520643215206163No Hit
ATACCATTTAGAATAGAAGTGAATAGGACACGGGTCATCAACTACATATG78560.10417213649944494No Hit
CTCAAAAGAAAGACAGAATGATTGAACTTTCATTAATTGACTTCTATTTG78270.1037875906798823No Hit
TAATTATGCTTATTATCTTTTGGTTCTCACTTGAACTGCAAGATCATAAT78210.10370802947583486No Hit
CGTCATGATACTCTAAAAAGTCTTCATAGAACGAACAACGCACTACAAGA78060.10350912646571629No Hit
ATTCACTTCTATTCTAAATGGTATATTAGAGTAGGAGCTAGAAAATCAGC78020.10345608566301799No Hit
GGTTTATGATGTAATCAAGATTCCAAATGGAAACTTTAAAAGTCCTCATA77320.10252787161579788No Hit
GTGTTAATTGGAACGCCTTGTCCTCGAGGGAATTTAAGGTCTTCCTTGCC77260.10244831041175044No Hit
TCGATGTACTGAATGGGTGATTTAGAACCAGCCTCATCCACGCACAATTC77030.10214332579623527No Hit
GGACACGGGTCATCAACTACATATGGTTGATGTTGAGTACATGACTGTAA76990.10209028499353699No Hit
GAAGTGAATAGGACACGGGTCATCAACTACATATGGTTGATGTTGAGTAC75590.10023385689909677No Hit
ATTTTACCGTCACCACCACGAATTCGTCTGGTAGCTCTTCGGTAGTAGCC75580.1002205966984222No Hit
GTTTTGATCGCGCCCCACTGCGTTCTCCATTCTGGTTACTGCCAGTTGAA75550.10018081609639848No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCCTA69100.0137.502431
TCCTAGG81700.0117.174183
CAGGTAT6650.0115.514194
TTCCTAG82300.0115.0917362
CCTAGGT83400.0114.4381564
CTAGGTA87550.0109.3454365
TAGGTAG89100.0107.524586
GCAGGTA8050.095.4247743
AGGTAGT111650.085.8709957
GACCGAG851.70985E-1068.208652
GTTCTAG5100.065.3692251
GGACCGA1009.094947E-1265.227121
AGGTATA11950.063.6759455
AAATTAG49250.063.1255385
TTCTAGG5000.062.3256532
TCCAGGT2100.062.1161233
TCCGGCA350.003573494762.116123
CCTCGCC1102.5465852E-1159.2950252
TATGCGC12450.058.2082675
TGCGCTA12800.056.0493557
Produced by FastQC (version 0.11.5)