FastQCFastQC Report
Sat 25 Apr 2020
HNLNCBGXF_n01_CV-40_B.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLNCBGXF_n01_CV-40_B.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4252583
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCT57623413.550211718383862No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGT53941612.684432026370796No Hit
CTCCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCAT3433248.073305094809436No Hit
TTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACA1694543.984731162213648No Hit
CCCTAGCATTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTT804251.8912035344166123No Hit
GGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAAT701771.6502205835841415No Hit
TTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTCACCA587941.3825479714328914No Hit
GAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCT533851.2553546867868306No Hit
ATATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTC532901.2531207503768884No Hit
AGCATTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACA455221.0704552974039543No Hit
CTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCTGT447941.0533362899677678No Hit
CTCCCTAGCATTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGG370020.8701064741123218No Hit
GGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGG365750.8600655178276356No Hit
GTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATG350340.8238287177463672No Hit
ATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCAC324470.7629951020356334No Hit
GGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGCTGTCTCTTATAC290190.6823852703168873No Hit
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCCTGTCTCTTATAC269280.6332151541780607No Hit
CTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACA268890.6322980644939793No Hit
GTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTCACCAAT251770.5920401788748155No Hit
GCATTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAG246070.5786365604151642No Hit
CTCCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCCTGTCTCTTAT199030.4680214354428826No Hit
CAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGG197390.4641649557457197No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGACTGTCTCTTATAC197200.4637181684637314No Hit
GCTCTCCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCG196940.4631067753410104No Hit
GGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGAGCTGTCTCTTAT192070.45165491185004497No Hit
TCTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCAATG189520.44565855622335887No Hit
GCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTCA187460.44081444148180055No Hit
TCTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCTGTCTCTTATAC176070.41403071968260236No Hit
GTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGCTGTCTCTTATACA153420.36076897264556623No Hit
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTCTCTTATACA149990.3527032864496707No Hit
ATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTCACC146040.3434148140083333No Hit
TGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGCTG136640.32131060110996074No Hit
CTCCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTCTCTTATA123180.28965924944909954No Hit
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCACTG120440.28321610654042495No Hit
GTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGAGCTGTCTCTTATA119640.2813348969320528No Hit
AGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTC116590.27416278530013405No Hit
GATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGC110110.25892498747231973No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACCTGT108800.2558445067386104No Hit
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCC98350.23127120622924938No Hit
GTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCTGT89030.209355114291714No Hit
CCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCC84450.19858518928378352No Hit
TGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGAGC81630.19195392541427175No Hit
CTCCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCAC79730.18748605259438791No Hit
GTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGG78900.18553429762570184No Hit
GCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGCTGT74170.17441164581620158No Hit
CCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTGAATATTGGTGA72640.17081383244018988No Hit
CTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCAGGCACCCAATGC71660.16850935066993403No Hit
TCTCCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCA67700.15919736310849195No Hit
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCCTGT67450.15860948510587566No Hit
GATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGA66870.15724560813980584No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGCTGCGCAAACAGGT65540.15411809716588717No Hit
CAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGCTGTCTCTTAT64330.1512727676332243No Hit
CCAATATTCCAGGCACCCAATGCTAGGGAGAGCTGCCTAGCATTGTGAAC62850.1477925298577359No Hit
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGCTGTCTCTTAT62590.14718113673501493No Hit
CCCTAGCATTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACCTGTC60290.14177265911094505No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGCTGTCTCTTATACA59570.1400795704634101No Hit
GAATATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGC59450.1397973890221543No Hit
GTTTGCGCATCTGTTCACCAATATTCCAGGCACCCAATGCTAGGGCTGTC55950.1315670969855262No Hit
GAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATCTGTTCACCAATAT55870.13137897602468898No Hit
GCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAA55670.13090867362259595No Hit
GCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGCTGTCT55530.13057946194113085No Hit
CTCCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCCT54840.12895691865390987No Hit
CTGTGAACCTGTTTGCGCATCTGTTCACCAATATTCCTGTCTCTTATACA52950.12451256095413071No Hit
CTAGCATTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAACAGGTTCA52800.12415983415256092No Hit
CTCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCGCATC52590.12366601663036324No Hit
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGAACCTGTTTGCCTGTCT52120.12256080598544461No Hit
GCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAGGGAGCT52110.12253729086533996No Hit
GAATATGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTT49950.1174580249227352No Hit
GCTCTCCCTAGCATTGGGTGCCTGGAATATTGGTGAACAGATGCGCAAAC48930.11505948267206072No Hit
ACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACAATGCTAG47600.11193197169814205No Hit
CCCAATGCTAGGGAGAGCTGCCTAGCATTGGGTGCCTGGAATATTGGTGA45410.10678216039522333No Hit
CCCTAGCATTGTGAACCTGTTTGCGCATCTGTGCACCTGTTTGCGCATCT43880.10318434701921161No Hit
TTGGTGAACAGATGCGCAAACAGGTTCACAGATGCGCAAACAGGTTCACC42620.1002214418860255No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACC100.0071015633144.932399
CTCCCTA652600.0117.997681
TCCCTAG655650.0117.451542
GGATATT6350.099.297231
AGCGAAC301.6034899E-596.6254
CCTCGCA950.091.541622
TCGCATT950.091.539474
CATGAAG2550.090.949721
TGAAGAC2650.087.509433
GTAAACC6300.086.272323
GAATATT1163850.085.356581
AATATTG1172550.084.779082
CCCTAGC1561750.083.7871861
CTAATGT6250.083.491851
TATTGGT1206350.082.624054
CCCTCGC1150.081.9288941
GATATTG7800.081.7615362
CCTAGCA1620400.080.96352
TAATGTT6450.080.897252
ATATTGG1239900.080.5523
Produced by FastQC (version 0.11.5)